Identification of phosphate solubilizing bacteria in a Andisol of Colombian coffee region
subject: Bacillus subtilis, Kocuria sp, biofertilizantes, eficiencia solubilizadora de fosfato, Ácidos orgânicos
description: Phosphorus is an essential nutrient for coffee growing, however, in Colombia most of the soils have low concentrations of this element. A strategy to supply the demand is the use of phosphate solubilizing microorganisms (PSM), in that order, 26 rhizospheric bacteria of Typic melanudans soil of Cajibío (Cauca, Colombia) were isolated, in three agroecosystems: coffee at full sun exposure, coffee with shade and relic secondary forest, evaluating the phosphate solubilizing efficiency (PSE) for Ca-P, Al-P, Fe-P in the solid and liquid Pikovskaya media. It showed the following solubilization sequence: Ca-P> Al-P> Fe-P. Two isolated bacteria were identified by DNA extraction and analysis of the 16S rRNA gene as Kocuria sp, and Bacillus subtilis. Later, using HPLC were identified organic acids present in the three phosphorus sources as: citric, gluconic, D- and L-malic, D- and L-lactic acid with higher presence in Ca-P-Kocuria sp. Strong acidification was observed for Fe-P and Al-P in all conditions.
creator: Carlos Adolfo Cisneros Rojas, Marina Sánchez de Prager, Juan Carlos Menjivar Flores
source: Revista Colombiana de Biotecnología
publisher: Universidad Nacional de Colombia
date: 2017
relation: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/65966
coverage: Biotechnology