West Nile Virus, direct sequencing, Whole genome amplification, SARS-CoV, Emerging infectious virus, rapid determination system
We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.
Tomohiko Takasaki, Hidekazu Nishimura, Masayuki Saijo, Shigeru Morikawa, Shuetsu Fukushi, Tetsuya Mizutani, Koji Ishii, Mikako Ito, Tetsuro Suzuki, Ichiro Kurane, Chang-Kweng Lim, Hiroyuki Shimizu, Minetaro Arita, Michiko Okamoto, Kazuya Shirato, Daiji Endoh, Reiko Nerome
Centers for Disease Control and Prevention
Infectious and parasitic diseases, Medicine