EFEITO DO EXTRATO AQUOSO DE PRÓPOLIS MARROM SOBRE A PRODUÇÃO DE IFN-γ APÓS IMUNIZAÇÃO CONTRA PARVOVÍRUS CANINO (CPV) E CORONAVÍRUS CANINO (CCoV)
subject: adjuvant, canine coronavirus, canine parvovirus, vaccine
description: The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-γ. Mice were vaccinated with CPV and CCoV (3.0 x 106 TCID50) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-γ mRNA in the immunized animals. Increased levels of IFN-γ mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 μg/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-γ to CPV were not influenced by the presence of WEBP.
creator: Maureen Hoch Vieira Fernandes, Lílian das Neves Ferreira, Gilberto D'Ávila Vargas, Geferson Fischer, Silvia de Oliveira Hübner
source: Ciência Animal Brasileira
publisher: Universidade Federal de Goiás
date: 2015
language: EN, PT
identifier: DOI: 10.1590/1089-6891v16i223458
coverage: Agriculture, Animal culture