Immunofluorescence Assay for Serologic Diagnosis of SARS
coronavirus, diagnosis, serology, Sensitivity, specificity, severe acute respiratory syndrome
We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
Paul K. S. Chan, King-Cheung Ng, Rickjason C. W. Chan, Rebecca K. Y. Lam, Viola C. Y. Chow, Mamie Hui, Alan Wu, Nelson Lee, Florence H.Y. Yap, Frankie W. T. Cheng, Joseph J. Y. Sung, John S. Tam
Centers for Disease Control and Prevention
Infectious and parasitic diseases, Medicine