Immunofluorescence Assay for Serologic Diagnosis of SARS
subject: coronavirus, diagnosis, serology, Sensitivity, specificity, severe acute respiratory syndrome
description: We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
creator: Paul K. S. Chan, King-Cheung Ng, Rickjason C. W. Chan, Rebecca K. Y. Lam, Viola C. Y. Chow, Mamie Hui, Alan Wu, Nelson Lee, Florence H.Y. Yap, Frankie W. T. Cheng, Joseph J. Y. Sung, John S. Tam
source: Emerging Infectious Diseases
publisher: Centers for Disease Control and Prevention
date: 2004
language: EN
identifier: DOI: 10.3201/eid1003.030493
coverage: Infectious and parasitic diseases, Medicine