1037
10
20655
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https://www.socictopen.socict.org/files/original/b61d7e09da46ec3106b01c5c103daa22.pdf
6a20d95e2b01dcecb32622193ca02c96
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identificación de variedades de papa nativa (Solanum sp.) producidas en tres comunidades del municipio de Tiahuanaco
Creator
An entity primarily responsible for making the resource
Lindsay Bustillos Siñani, Abel Rojas Pardo, Celia Fernández Chávez, Juan José Aparicio Porres
Description
An account of the resource
En varias regiones del Altiplano boliviano, el comportamiento climático está cambiando, influyendo sobre la producción agrícola de los cultivos que se producen en la región. Actualmente la producción de papa enfrenta factores adversos como la disponibilidad y cantidad de semilla de variedades nativas que tienen rendimientos menores, sumada a la presencia de plagas y enfermedades, helada, granizada y sequia provocan la disminución de la producción de papa nativa, con riesgo a desaparecer. El estudio se realizó en la provincia Ingavi del municipio de Tiahuanaco del departamento de La Paz en las comunidades de Caluyo, Pillapi y Huacuyo. El presente trabajo de investigación identifica las variedades nativas producidas en los sistemas de producción agrícola existentes en las tres comunidades, para esto, se obtuvo información primaria por medio de visitas a campo, entrevistas y encuestas a 54 productores de estas comunidades, como parte del método no aleatorio (muestreo por conveniencia). De nueve variedades nativas producidas en las tres comunidades, cuatro son de mayor importancia que son: Imilla Negra (100% de productores), por ser consumida como papa hervida y en puré, Surimana y Saq'anpaya (74% de productores), por ser consumidas como papa hervida o chuño; la variedad Wila Pala (63% de productores) consumida como papa hervida y puré, variedad Sani Imilla (52% de productores) consumida como papa hervida o papa frita, variedad Waych'a (46% de productores) consumida como papa hervida o puré, variedad Luk'i (41% de productores) consumida como chuño y tunta y Khati Señorita (26% de productores) consumida como chuño. Estas papas nativas contribuyen a la seguridad alimentaria de la región por la frecuencia de consumo y cultivo que presentan.
Date
A point or period of time associated with an event in the lifecycle of the resource
2018
Subject
The topic of the resource
Producción, identificación, papa nativa, seguridad alimentaria
Source
A related resource from which the described resource is derived
Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales
Publisher
An entity responsible for making the resource available
Universidad Mayor de San Andrés
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Agriculture (General), Plant culture
Relation
A related resource
<a href="http://riiarn.agro.umsa.bo/index.php/RIIARn/article/view/120" target="_blank" rel="noreferrer noopener">http://riiarn.agro.umsa.bo/index.php/RIIARn/article/view/120</a>
-
https://www.socictopen.socict.org/files/original/638fe67776297cf546df46a01f62912d.pdf
d61a523c5fcddad4e4e0d4ac4402cd9d
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identificación de vulnerabilidades y clasificación de unidades de producción lechera mediante el sistema PRAGACC
Creator
An entity primarily responsible for making the resource
Zoe G. Acosta Gutiérrez, Guillermo E. Guevara Viera, José M. Plasencia Fraga, Jorge Pereda Mouso
Description
An account of the resource
Mediante el sistema PRAGACC se identificaron las principales vulnerabilidades y se clasificaron las 14 vaquerías de la unidad de producción cooperativa (UBPC) Patria o Muerte en Camagüey, Cuba, con miras a la aplicación de medidas de mitigación. En el análisis de las entidades todas manifestaron problemas al manejar elementos del ecosistema (subsistema I, recursos naturales); de ellas, cinco mostraron afectaciones en dos variables, seis en tres y tres en cuatro. Dentro de los principales problemas identificados en este subsistema se destaca la baja disponibilidad y el uso inadecuado del agua, la escasa presencia de árboles en los pastizales y las insuficientes fuentes endógenas como alternativas para alimentar al ganado. En relación con el subsistema II (otros recursos) se encontró que seis entidades manifestaron insuficiencias en el proceso productivo, vinculadas principalmente con la falta de iniciativas en la obtención de fuentes de energía y el desaprovechamiento de materiales para la producción de bioabonos. El sistema permitió identificar dos entidades más afectadas, que deben tener prioridad al aplicar las medidas. PRAGACC también posibilitó dar orden de prioridad a las restantes vaquerías.
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
Subject
The topic of the resource
cambios climáticos y adaptación, sostenibilidad, ulnerabilidades en fincas ganaderas
Source
A related resource from which the described resource is derived
Revista de Producción Animal
Publisher
An entity responsible for making the resource available
Ediciones Universidad de Camagüey
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Animal culture
Relation
A related resource
<a href="https://revistas.reduc.edu.cu/index.php/rpa/article/view/2536" target="_blank" rel="noreferrer noopener">https://revistas.reduc.edu.cu/index.php/rpa/article/view/2536</a>
-
https://www.socictopen.socict.org/files/original/389b0a3128a0811d07b88507e7994c91.pdf
bebab225b993dc4bd676d58f0e8a73e6
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identificación microbiológica de marchitez vascular en col china (Brassica campestris L. var pekinensis) en Cogua, Colombia
Creator
An entity primarily responsible for making the resource
Ligia Sánchez, Yesid Ariza, Estefania Castañeda, Edgar Martinez
Description
An account of the resource
La col china (Brassica campestris L. var pekinensis) es una hortaliza de origen asiático apreciada en todos los continentes por su valor nutricional y propiedades medicinales. Reportes de productores de todo el país han manifestado que hay pérdidas económicas en este cultivo al parecer originadas por enfermedades bacterianas; una de ellas es la marchitez bacteriana, que disminuye el tiempo de viabilidad del producto, también su continuidad en el mercado y, en consecuencia, la no utilización del mismo por parte del consumidor. Cultivadores en Cogua, Colombia reportan pérdidas de un 80% en su producción a causa de esta patología. La principal manifestación es la presencia de lesiones húmedas y marchitez en los bordes externos de las hojas. El objetivo de este proyecto fue la identificación microbiológica de los posibles patógenos de la marchitez bacteriana en un cultivo de col china en Cogua, Colombia. La metodología utilizada incluyó el análisis de los microorganismos presentes en plantas sanas y enfermas, el aislamiento de bacterias de las lesiones vasculares, la elaboración de pruebas de patogenicidad y la identificación fenotípica del patógeno. Los resultados permitieron concluir que el microorganismo patógeno que ocasionaba marchitez bacteriana en los cultivos de col china en Cogua, Colombia era Xanthomonas axonopodis. La identificación de patógenos en plantas de interés económico permite tomar medidas más eficientes referentes a las buenas prácticas agrícolas para manejar enfermedades en plantas y disminuir las pérdidas de producción.
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
Subject
The topic of the resource
Col china, Flavonoides, Xanthomonas, fitopatología, marchitez vascular
Identifier
An unambiguous reference to the resource within a given context
10.22490/24629448.447
Source
A related resource from which the described resource is derived
NOVA
Publisher
An entity responsible for making the resource available
Universidad Colegio Mayor de Cundinamarca
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Biology (General), Medicine (General)
Relation
A related resource
<a href="https://revistas.unicolmayor.edu.co/index.php/nova/article/view/158" target="_blank" rel="noreferrer noopener">https://revistas.unicolmayor.edu.co/index.php/nova/article/view/158</a>
-
https://www.socictopen.socict.org/files/original/17c2b09bcacfeb44acb097be07fd6894.pdf
6c2b66f93e2ad39bd949227edb1aeb07
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identificación molecular de cepas de Bacillus spp. y su uso como rizobacteria promotora del crecimiento en tomate (Lycopersicum esculentum Mill.)
Creator
An entity primarily responsible for making the resource
Ramiro Daniel Acurio Vásconez, Estefany Michelle Tenorio Moya, Katherine Alejandra Medrano Jara, Viviana Pamela Chiluisa-Utreras
Description
An account of the resource
La aplicación de rizobacterias promotoras de crecimiento vegetal (RPCV), constituye una práctica ecológica que contribuye a optimizar los sistemas de producción agrícola y se presenta como una opción para la reducción del uso de fertilizantes químicos. El tomate riñón tiene alta demanda alrededor de todo el mundo, su producción requiere una nutrición eficiente para suplir la demanda de cantidad y calidad que el mercado requiere, por lo tanto, en la presente investigación se evaluaron dos cepas bacterianas: Bacillus licheniformis (IB10) y Bacillus megaterium (CT11) como promotoras del crecimiento en plántulas de tomate riñón mediante pruebas en semilleros. Se evidenció diferencias estadísticas en las distintas variables evaluadas, la aplicación de B. licheniformis únicamente al momento de la siembra provocó un incremento del 10% en el grosor del tallo y un aumento del 100% en cantidad de biomasa seca, la aplicación semanal de B. megaterium aumentó la eficiencia fotosintética en un 18% y aumentó en un 11% la longitud de las raíces de las plántulas. Los resultados generados permiten determinar que ambas cepas bacterianas pueden ser utilizadas como alternativas a fertilizantes químicos.
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
Subject
The topic of the resource
Bacillus, biofertilizante, promotor, rizobacterias promotoras del crecimiento en plantas, rizobactéria
Identifier
An unambiguous reference to the resource within a given context
10.17268/sci.agropecu.2020.04.13
Source
A related resource from which the described resource is derived
Scientia Agropecuaria
Publisher
An entity responsible for making the resource available
Universidad Nacional de Trujillo
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Agriculture (General), Agriculture
Relation
A related resource
<a href="https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3170" target="_blank" rel="noreferrer noopener">https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3170</a>
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https://www.socictopen.socict.org/files/original/ea2a509fd83c7c8e5dc4fa7be5ee6089.pdf
f18aff82de0766b5c3a69602f0ddea11
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identificación morfológica, morfométrica y molecular de Meloidogyne incognita en higuera (Ficus carica L.) en Costa Rica.
Creator
An entity primarily responsible for making the resource
Walter Peraza-Padilla, Johaner Rosales-Flores, Alejandro Esquivel-Hernández, Irena Hilje-Rodríguez, Ramón Molina-Bravo, Pablo Castillo-Castillo
Description
An account of the resource
El objetivo de este estudio fue identificar la especie de Meloidogyne asociada a dos plantaciones de higuera en Costa Rica. En marzo de 2012, en el distrito de Pacayas, provincia de Cartago, se detectaron agallas en los sistemas radicales de plantas de higuera (Ficus carica L.) de dos fincas. De las raíces agalladas se extrajeron hembras, masas de huevos y juveniles (J2) de Meloidogyne sp. Se examinaron los patrones perineales de las hembras y los segundos estadios infectivos se analizaron morfológica y molecularmente mediante PCR-RFLP. Se amplificó la región intergénica (IGS) del genoma mitocondrial, delimitada por el gen de la subunidad II de la citocromo oxidasa (COII) y el gen ribosomal 16S. La población de nematodos se identificó como M. incognita. El tamaño de los productos de PCR generados con los imprimadores C2F3 y 1108 fue de 1,7 kb. Al tratar los productos de PCR con enzimas de restricción, se generaron cuatro fragmentos de 850, 450, 250 y 150 pb con la enzima AluI y dos fragmentos de 1300 y 400 pb con la enzima HinfI.
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
Subject
The topic of the resource
nematodo agallador, patrones perineales, taxonomía de nematodo
Identifier
An unambiguous reference to the resource within a given context
10.15517/am.v24i2.12533
Source
A related resource from which the described resource is derived
Agronomía Mesoamericana
Publisher
An entity responsible for making the resource available
Universidad de Costa Rica
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Agriculture
Relation
A related resource
<a href="https://revistas.ucr.ac.cr/index.php/agromeso/article/view/12533" target="_blank" rel="noreferrer noopener">https://revistas.ucr.ac.cr/index.php/agromeso/article/view/12533</a>
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https://www.socictopen.socict.org/files/original/259a9b6f652c069f998b39e505e47e0a.pdf
e7b10520468e91590c66e477329c22f5
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identificando os determinantes da inadimplência contratual no Programa Agroamigo Crescer
Creator
An entity primarily responsible for making the resource
Emanoel Márcio Nunes, Fábio Lúcio Rodrigues, Camila Escobar
Description
An account of the resource
O objetivo do trabalho é identificar determinantes da inadimplência dos tomadores de financiamento do Programa Agroamigo Crescer Pecuária, no período de 2005 a 2017, atribuindo probabilidades a cada fator relacionado. Como metodologia, dentre as diversas variações dos modelos de atribuição de probabilidades, este trabalho utiliza o modelo LOGIT de resposta binária. Os resultados obtidos para o modelo de inadimplência estimado apontam para o sentido correto das relações empíricas percebidas. Temos que o aumento do valor do contrato aumenta a chance do tomador se tornar inadimplente e que o aumento do prazo reduzirá essa possibilidade. Enquanto que a finalidade direcionada a investimentos, essa a maior proporção dos contratos, aumentara o risco da inadimplência.
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
Subject
The topic of the resource
Agricultura Familiar, Inadimplência, microcrédito, pecuária
Identifier
An unambiguous reference to the resource within a given context
10.5007/2177-5230.2021.e72362
Source
A related resource from which the described resource is derived
Geosul
Publisher
An entity responsible for making the resource available
Universidade Federal de Santa Catarina
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Geography (General), Human ecology. Anthropogeography, Physical geography
Relation
A related resource
<a href="https://periodicos.ufsc.br/index.php/geosul/article/view/72362" target="_blank" rel="noreferrer noopener">https://periodicos.ufsc.br/index.php/geosul/article/view/72362</a>
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https://www.socictopen.socict.org/files/original/1f86ee13d630b4be8dcecdf60b19f5a3.pdf
8108ad18f5136aad8865c9156b7eb2ca
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Agricultura sostenible
Description
An account of the resource
Dominio científico: Agricultura sostenible
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification and Assessment of Livestock Best Management Practices (BMPs) Using the REDD+ Approach in the Ecuadorian Amazon
Creator
An entity primarily responsible for making the resource
Bolier Torres, David Eche, Yenny Torres, Carlos Bravo, Christian Velasco, Antón García
Description
An account of the resource
Deforestation is a severe threat to diversity in the Ecuadorian Amazon Region (EAR). To mitigate deforestation, it is necessary to know the relevant stakeholders’ roles and interactions and deepen our knowledge of the local livelihoods, objectives, potentials, limitations, and “rights of being” among farms, as well as the best management practices (BMPs). In this study, our aim was to identify and assess livestock BMPs along an elevational gradient to foster sustainable production and reduce emissions from deforestation and forest degradation (REDD+). This approach could be environmentally and economically beneficial. Data were collected from 167 households along three elevational gradients, as well as from 15 interviews held among a multidisciplinary panel of key stakeholders and researchers in the EAR. The results showed that most of the Kichwa population lives in the medium zone, which features a larger agricultural and forest surface. Conversely, in the lower and upper areas, livestock predominates, where the upper area is specialized in milk production and the lower area in dual-purpose cattle (meat and dairy). The stakeholder assessment provided several key results: (a) social, structural, and technical factors have complementary effects on BMP adoption; (b) the sixteen assessed BMPs facilitated the implementation of existing financial incentive programs and enabled public–private partnerships to develop REDD+ projects. The policy implications of implementing these approaches are also discussed.
Date
A point or period of time associated with an event in the lifecycle of the resource
2021
Subject
The topic of the resource
Ecuadorian Amazon, REDD+, biosphere reserve, pasture, restoration
Identifier
An unambiguous reference to the resource within a given context
10.3390/agronomy11071336
Source
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Agronomy
Publisher
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MDPI AG
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Agriculture
Relation
A related resource
<a href="https://www.mdpi.com/2073-4395/11/7/1336" target="_blank" rel="noreferrer noopener">https://www.mdpi.com/2073-4395/11/7/1336</a>
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https://www.socictopen.socict.org/files/original/9aea7f12e086f2f335542127cff27a74.pdf
9a66c41751e58697d5d020803b64023c
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Coronavirus
Description
An account of the resource
Dominio científico: Coronavirus
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
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Title
A name given to the resource
Identification and Characterization of a Novel Alpaca Respiratory Coronavirus Most Closely Related to the Human Coronavirus 229E
Creator
An entity primarily responsible for making the resource
Sharon K. Hietala, Richard E. Mock, Scott A. Callison, Beate M. Crossley
Description
An account of the resource
In 2007, a novel coronavirus associated with an acute respiratory disease in alpacas (Alpaca Coronavirus, ACoV) was isolated. Full-length genomic sequencing of the ACoV demonstrated the genome to be consistent with other Alphacoronaviruses. A putative additional open-reading frame was identified between the nucleocapsid gene and 3'UTR. The ACoV was genetically most similar to the common human coronavirus (HCoV) 229E with 92.2% nucleotide identity over the entire genome. A comparison of spike gene sequences from ACoV and from HCoV-229E isolates recovered over a span of five decades showed the ACoV to be most similar to viruses isolated in the 1960&#8217;s to early 1980&#8217;s. The true origin of the ACoV is unknown, however a common ancestor between the&#160;ACoV and HCoV-229E appears to have existed prior to the 1960&#8217;s, suggesting virus&#160;transmission, either as a zoonosis or anthroponosis, has occurred between alpacas and humans.
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
Subject
The topic of the resource
alpaca, human, coronavirus, reverse zoonosis, anthroponosis, respiratory, molecular evolution
Identifier
An unambiguous reference to the resource within a given context
DOI: 10.3390/v4123689
Source
A related resource from which the described resource is derived
Viruses
Publisher
An entity responsible for making the resource available
MDPI AG
Coverage
The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant
Microbiology
Language
A language of the resource
EN
-
https://www.socictopen.socict.org/files/original/e2e54cdcc9ceefb338f67aae13fe33ed.pdf
d4cd14f87122973e4b75d4313d8de449
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Coronavirus
Description
An account of the resource
Dominio científico: Coronavirus
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification and Characterization of Three Novel Small Interference RNAs That Effectively Down-Regulate the Isolated Nucleocapsid Gene Expression of SARS Coronavirus
Creator
An entity primarily responsible for making the resource
Yingli Cao, Li Liu, Shu-Hui Wang, Yun Zhang, Fan Yang, Rong Guo, Ying Wang
Description
An account of the resource
Nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major pathological determinant in the host that may cause host cell apoptosis, upregulate the proinflammatory cytokine production, and block innate immune responses. Therefore, N gene has long been thought an ideal target for the design of small interference RNA (siRNA). siRNA is a class of small non-coding RNAs with a size of 21-25nt that functions post-transcriptionally to block targeted gene expression. In this study, we analyzed the N gene coding sequences derived from 16 different isolates, and found that nucleotide deletions and substitutions are mainly located at the first 440nt sequence. Combining previous reports and the above sequence information, we create three novel siRNAs that specifically target the conserved and unexploited regions in the N gene. We show that these siRNAs could effectively and specifically block the isolated N gene expression in mammal cells. Furthermore, we provide evidence to show that N gene can effectively up-regulate M gene mediated interferon b (IFNb) production, while blocking N gene expression by specific siRNA significantly reduces IFNb gene expression. Our data indicate that the inhibitory effect of siRNA on the isolated N gene expression might be influenced by the sequence context around the targeted sites.
Date
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2011
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siRNA, SARS-CoV, nucleocapsid gene, RT-PCR, EGFP
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DOI: 10.3390/molecules16021544
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Molecules
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MDPI AG
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Organic chemistry
Language
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EN
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https://www.socictopen.socict.org/files/original/eac40db9c604f95c563710d1653962bd.pdf
6776decfb37131f66e3b4a7d0ed728e0
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Coronavirus
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Dominio científico: Coronavirus
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Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains
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Feng Deng, Gang Ye, Qianqian Liu, Muhammad Tariq Navid, Xiaoli Zhong, Youwen Li, Chunyun Wan, Shaobo Xiao, Qigai He, Zhen F. Fu, Guiqing Peng
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Porcine epidemic diarrhea virus (PEDV), a member of Alphacoronavirus, has caused huge economic losses for the global pork industry recently. The spike (S) protein mediates PEDV entry into host cells. Herein, we investigated the interactions between the S protein and its receptor porcine aminopeptidase N (pAPN) or co-receptor sugars. The C-terminal domain (CTD) of the S1 domain is bound to pAPN. The prototype strain demonstrated similar receptor-binding activity compared with the variant field isolate. Three loops at the tips of the β-barrel domains did not play crucial roles in the PEDV S-pAPN association, indicating that PEDV conforms to a different receptor recognition model compared with transmissible gastroenteritis virus (TGEV), porcine respiratory CoV (PRCV), and human coronavirus NL63 (HCoV-NL63). The N-terminal domain (NTD) of the PEDV S1 domain could bind sugar, a possible co-receptor for PEDV. The prototype strain exhibited weaker sugar-binding activity compared with the variant field isolate. Strategies targeting the receptor binding domain (RBD) may be helpful for developing vaccines or antiviral drugs for PEDV. Understanding the differences in receptor binding between the prototype and the variant strains may provide insight into PEDV pathogenesis.
Date
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2016
Subject
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PEDV, S protein, RBD, pAPN, CV777 strain, CHGD-01 strain, sugar
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DOI: 10.3390/v8030055
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Viruses
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MDPI AG
Coverage
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Microbiology
Language
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EN