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                  <text>Dominio científico: Coronavirus</text>
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                <text>A chimeric HS4-SAR insulator (IS2) that prevents silencing and enhances expression of lentiviral vectors in pluripotent stem cells.</text>
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                <text>Karim Benabdellah, Alejandra Gutiérrez-Guerrero, Marien Cobo, Pilar Muñoz, Francisco Martín</text>
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                <text>Chromatin insulators, such as the chicken β-globin locus control region hypersensitive site 4 (HS4), and scaffold/matrix attachment regions (SARs/MARs) have been incorporated separately or in combination into retroviral vectors (RVs) in order to increase transgene expression levels, avoid silencing and reduce expression variability. However, their incorporation into RVs either produces a reduction on titer and/or expression levels or do not have sufficient effect on stem cells. In order to develop an improved insulator we decided to combine SAR elements with HS4 insulators. We designed several synthetic shorter SAR elements containing 4 or 5 MAR/SARs recognition signatures (MRS) and studied their effects on a lentiviral vector (LV) expressing eGFP through the SFFV promoter (SE). A 388 bp SAR element containing 5 MRS, named SAR2, was as efficient or superior to the other SARs analyzed. SAR2 enhanced transgene expression and reduced silencing and variability on human embryonic stem cells (hESCs). We next compared the effect of different HS4-based insulators, the HS4-Core (250 bp), the HS4-Ext (400 bp) and the HS4-650 (650 bp). All HS4 elements reduced silencing and expression variability but they also had a negative effect on transgene expression levels and titer. In general, the HS4-650 element had a better overall effect. Based on these data we developed a chimeric insulator, IS2, combining the SAR2 and the HS4-650. When incorporated into the 3' LTR of the SE LV, the IS2 element was able to enhance expression, avoid silencing and reduce variability of expression on hESCs. Importantly, these effects were maintained after differentiation of the transduced hESCs toward the hematopoietic linage. Neither the HS4-650 nor the SAR2 elements had these effects. The IS2 element is therefore a novel insulator that confers expression stability and enhances expression of LVs on stem cells.</text>
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                <text>DOI: 10.1371/journal.pone.0084268</text>
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                <text>PLoS ONE</text>
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                  <text>Agricultura sostenible</text>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>Capacitación de productores y manipuladores de alimentos en seguridad alimentaria</text>
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                <text>Karim Yamul, Mariana Dulout, Guido Mastrantonio, Pedro Zeinsteger</text>
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                <text>Las enfermedades transmitidas por alimentos (ETA) implican un riesgo para la población susceptible debido a errores en la manipulación, almacenamiento o servicio. Las pérdidas ocasionadas, por fallas en los aspectos sanitarios durante la producción, se estiman en el mundo en el orden del 30%. Existe la necesidad de facilitar la incorporación de conocimientos teóricos y prácticos relacionados con la producción segura de alimentos en circuitos formales e informales de producción. Tradicionalmente, dichos conocimientos se transmitían entre especialistas de oficio, de maestros a aprendices, de un establecimiento a otro, o bien entre gremios. La realidad muestra una pérdida progresiva de estas tradiciones y el aumento de producciones de alimentos de manera ineficaz con el consiguiente riesgo para la salud. Las normativas vigentes y las dependencias del Estado encargadas de impartirlas han prestado escasa atención a las metodologías didácticas para capacitar trabajadores alfabetizados o no en el área de seguridad alimentaria. El presente proyecto de extensión ha tenido como eje central la interacción horizontal y en ambos sentidos entre capacitadores y capacitandos, compartiendo aspectos teóricos y experiencias personales, con el propósito de construir metodologías didácticas adecuadas y marcos de trabajo consistentes con nuevas y mejores alternativas en políticas de salud pública.   Palabras clave: seguridad alimentaria- capacitación- extensión</text>
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                <text>Extensión en Red</text>
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                <text>Universidad Nacional de La Plata</text>
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                <text>Social sciences (General), Communication. Mass media</text>
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                <text>&lt;a href="https://perio.unlp.edu.ar/ojs/index.php/extensionenred/article/view/944" target="_blank" rel="noreferrer noopener"&gt;https://perio.unlp.edu.ar/ojs/index.php/extensionenred/article/view/944&lt;/a&gt;</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Encephalopathy and Encephalitis Associated with Cerebrospinal Fluid Cytokine Alterations and Coronavirus Disease, Atlanta, Georgia, USA, 2020</text>
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                <text>Karima Benameur, Ankita Agarwal, Sara C. Auld, Matthew P. Butters, Andrew S. Webster, Tugba Ozturk, J. Christina Howell, Leda C. Bassit, Alvaro Velasquez, Raymond F. Schinazi, Mark E. Mullins, William T. Hu</text>
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                <text>There are few detailed investigations of neurologic complications in severe acute respiratory syndrome coronavirus 2 infection. We describe 3 patients with laboratory-confirmed coronavirus disease who had encephalopathy and encephalitis develop. Neuroimaging showed nonenhancing unilateral, bilateral, and midline changes not readily attributable to vascular causes. All 3 patients had increased cerebrospinal fluid (CSF) levels of anti-S1 IgM. One patient who died also had increased levels of anti-envelope protein IgM. CSF analysis also showed markedly increased levels of interleukin (IL)-6, IL-8, and IL-10, but severe acute respiratory syndrome coronavirus 2 was not identified in any CSF sample. These changes provide evidence of CSF periinfectious/postinfectious inflammatory changes during coronavirus disease with neurologic complications.</text>
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                <text>10.3201/eid2609.202122</text>
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                <text>Emerging Infectious Diseases</text>
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                <text>Medicine, Infectious and parasitic diseases</text>
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                <text>Air Microbiome and Pollution: Composition and Potential Effects on Human Health, Including SARS Coronavirus Infection</text>
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                <text>Karin Moelling, Felix Broecker</text>
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                <text>Polluted air poses a significant threat to human health. Exposure to particulate matter (PM) and harmful gases contributes to cardiovascular and respiratory diseases, including allergies and obstructive lung disease. Air pollution may also be linked to cancer and reduced life expectancy. Uptake of PM has been shown to cause pathological changes in the intestinal microbiota in mice and humans. Less is known about the effects of pollution-associated microbiota on human health. Several recent studies described the microbiomes of urban and rural air samples, of the stratosphere and sand particles, which can be transported over long distances, as well as the air of indoor environments. Here, we summarize the current knowledge on airborne bacterial, viral, and fungal communities and discuss their potential consequences on human health. The current data suggest that bacterial pathogens are typically too sparse and short-lived in air to pose a significant risk for infecting healthy people. However, airborne fungal spores may exacerbate allergies and asthma. Little information is available on viruses including phages, and future studies are likely to detect known and novel viruses with a yet unknown impact on human health. Furthermore, varying experimental protocols have been employed in the recent microbiome and virome studies. Therefore, standardized methodologies will be required to allow for better comparisons between studies. Air pollution has been linked to more severe outcomes of SARS (severe acute respiratory syndrome) coronavirus (SARS-CoV) infections. This may have contributed to severe SARS-CoV-2 outbreaks, especially those in China, Northern Italy, Iran, and New York City.</text>
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                <text>DOI: 10.1155/2020/1646943</text>
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                <text>Journal of Environmental and Public Health</text>
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                <text>Hindawi Limited</text>
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                <text>Public aspects of medicine</text>
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                <text>COMPARACIÓN DE LA ESTRUCTURA Y DE LA FUNCIÓN DE LOS MIEMBROS ANTERIOR Y POSTERIOR DE Cuniculus taczanowskii Y Dinomys branickii COMPARISON OF THE FORE AND HINDLIMB STRUCTURE AND FUNCTION OF Cuniculus taczanowskii AND Dinomys branickii</text>
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                <text>Las adaptaciones anatómicas que capacitan a los animales para desarrollarse de una manera específica en un ambiente determinado, se ven reflejadas en cambios morfológicos estructurales y fisiológicos. Cuniculus taczanowskii y Dinomys branickii son especies simpátricas, por lo cual,surgió la pregunta de si existen diferencias y similitudes en el esqueleto apendicular que expliquen sus hábitos locomotores, en el contexto de la ecología funcional. El análisis de las medidas óseas de los huesos largos que conforman el esqueleto apendicular muestra claras diferencias tanto inter como intraespecíficas, que permiten inferir características específicas de D. branickii y C. taczanowskii, durante la locomoción y, por lo tanto, durante los patrones de uso del hábitat. Los índices morfológicos corroboran las características anatómicas, que explica la capacidad para cavar propia de C. taczanowskii yel desplazamiento plantígrado durante la marcha lenta, característico de D. branickii.The anatomical adaptations that allow animals to develop in a specific way in a certain environment reflect structural and physiological changes. Cuniculus taczanowskii and Dinomys branickii are simpatric species, so the question arose if possible differences and similarities in the appendicular skeleton explain their locomotive habits in the context of functional ecology. The analysis of the long bone measures of the appendicular skeleton show inter and intraspecific differences that allow to assume specific characteristics of D. branickii and C. taczanowskii during the locomotion and therefore during the patterns of habitat use. The morphologic indices corroborate the anatomical characteristics that explain the fossorial activities of C. taczanowskii and the slow walking cursorial adaptation of D. branickii.</text>
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                <text>Las adaptaciones anatómicas que capacitan a los animales para desarrollarse de una manera específica en un ambiente determinado, se ven reflejadas en cambios morfológicos estructurales y fisiológicos. Cuniculus taczanowskii y Dinomys branickii son especies simpátricas, por lo cual,surgió la pregunta de si existen diferencias y similitudes en el esqueleto apendicular que expliquen sus hábitos locomotores, en el contexto de la ecología funcional. El análisis de las medidas óseas de los huesos largos que conforman el esqueleto apendicular muestra claras diferencias tanto inter como intraespecíficas, que permiten inferir características específicas de D. branickii y C. taczanowskii, durante la locomoción y, por lo tanto, durante los patrones de uso del hábitat. Los índices morfológicos corroboran las características anatómicas, que explica la capacidad para cavar propia de C. taczanowskii yel desplazamiento plantígrado durante la marcha lenta, característico de D. branickii.</text>
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                <text>Universidad de Ciencias Aplicadas y Ambientales</text>
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                <text>&lt;a href="https://revistas.udca.edu.co/index.php/ruadc/article/view/640" target="_blank" rel="noreferrer noopener"&gt;https://revistas.udca.edu.co/index.php/ruadc/article/view/640&lt;/a&gt;</text>
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                <text>RELATO DE UMA VIVÊNCIA NA ESCOLA FAMÍLIA AGRÍCOLA NOVA ESPERANÇA DO MUNICÍPIO DE TAIOBEIRAS/MG</text>
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                <text>Karina  Costa, Sandra Regina Gregório</text>
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                <text>Este trabalho teve como objetivo observar e vivenciar a rotina dos funcionários e jovens que estudam na Escola Família Agrícola Nova Esperança e entender a importância da mesma para suas vidas e para a região. É uma pesquisa de cunho qualitativo, utilizando para isto o método da observação participante, bem como análise documental e conversas informais com os sujeitos da pesquisa. Assim, iremos relatar um breve histórico do surgimento das EFAs no Brasil, em Minas Gerais e na região do Alto Rio Pardo. Em sequência, abordar sobre a pedagogia da alternância, metodologia utilizada pelas EFAs, que é uma forma de proporcionar aos jovens que vivem na zona rural uma educação de qualidade, valorizando os aspectos culturais, sociais, comunitários e políticos. Por fim, faremos um relato de uma vivência na EFA Nova Esperança, situada no município de Taiobeiras, microrregião do Alto Rio Pardo de Minas Gerais, onde destacaremos o apoio desta escola família agrícola para o desenvolvimento do conhecimento e habilidades desses alunos, contribuindo para que ao final do curso eles estejam capacitados e aptos a entrar tanto no mercado de trabalho, como também conscientes da importância de sua permanência em suas regiões, tornando-se assim referências e apoio para o crescimento e fortalecimento da agricultura familiar da região.</text>
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                <text>Educação do Campo, Pedagogia da Alternância, Vivência pedagógica</text>
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                <text>Universidade Federal de Minas Gerais</text>
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                <text>Special aspects of education, Labor. Work. Working class</text>
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                <text>Karina Colombera Peres, Rachel Riera, Rachel Riera, Ana Luiza Cabrera Martimbianco, Ana Luiza Cabrera Martimbianco, Laura Sterian Ward, Lucas Leite Cunha, Lucas Leite Cunha</text>
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                <text>A better understanding of the SARS-CoV-2 virus behavior and possible risk factors implicated in poor outcome has become an urgent need. We performed a systematic review in order to investigate a possible association between body weight and prognosis among patients diagnosed with COVID-19. We searched in Cochrane Library, EMBASE, MEDLINE, WHO-Global Literature on Coronavirus Disease, OpenGrey, and Medrxiv. We used the ROBINS-I tool or Cross-Sectional/Prevalence Study Quality tool from AHRQ, to evaluate the methodological quality of included studies. Nine studies (two prospective cohorts, four retrospective cohorts and three cross-sectional) were included and assessed the relationship between obesity and COVID-19 prognosis. Risk of bias of the included studies ranged from moderate to critical. Clinical and methodological heterogeneity among them precluded meta-analyses. Most of the included studies showed some degree of association to: (a) higher BMI and worse clinical presentation and (b) obesity and need of hospitalization. The results were inconsistent about the impact of obesity on mortality. Based on limited methodological quality studies, obesity seems to predict poor clinical evolution in patients with COVID-19. Further studies with appropriate prospective design are needed to reduce the uncertainty on this evidence.</text>
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                <text>10.3389/fendo.2020.00562</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>Diseases of the endocrine glands. Clinical endocrinology</text>
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                  <text>Dominio científico: Agricultura sostenible</text>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Carbono de biomasa microbiana influenciada por los residuos de cinco especies de abonos verdes sobre un suelo bajo uso pecuario</text>
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                <text>Karina Colque Arispe, Ing., David Ruiz-Alderete</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Se evaluó la influencia de residuos de abonos verdes (AV) sobre el Carbono (C) de la Biomasa microbiana del suelo (BMS) correspondiente a un área de uso pecuario (ganadero). El estudio fue desarrollado en el Proyecto Ganadería y Suelos del Centro de Investigación Agrícola Tropical (CIAT) Santa Cruz, Bolivia; el diseño experimental fue el de bloques completamente al azar, que contaba con 6 tratamientos y 4 repeticiones. La evaluación de la BMS fue a los 30 días de la incorporación de los residuos de las cinco especies de leguminosas empleadas como AV siendo Mucuna negra (Stizolobium aterrinum), Lab lab (Lablab purpureus), Mucuna ceniza (Stizolobium niveum),  Crotalaria (Crotalaria juncea),  Frejol de puerco (Canavalia ensiformis) y como testigo (vegetación espontánea) con el método de irradiación e incubación. Se encontró que las especies de AV tienen mayor concentración de C-BMS respecto al testigo, y entre los AV las especies de hábito rastrero son las que concentran mayor C en su BM.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2019</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="211718">
                <text>Carbono de biomasa microbiana, abono verde, leguminosas</text>
              </elementText>
            </elementTextContainer>
          </element>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="211719">
                <text>10.5377/ribcc.v5i10.8967</text>
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            </elementTextContainer>
          </element>
          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="211720">
                <text>Revista Iberoamericana de Bioeconomía y Cambio Climàtico</text>
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            </elementTextContainer>
          </element>
          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="211721">
                <text>Universidad Nacional Autónoma de Nicaragua</text>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="211722">
                <text>Economic theory. Demography, Meteorology. Climatology</text>
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            </elementTextContainer>
          </element>
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            <name>Relation</name>
            <description>A related resource</description>
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              <elementText elementTextId="211723">
                <text>&lt;a href="http://revistas.unanleon.edu.ni/index.php/REBICAMCLI/article/view/251" target="_blank" rel="noreferrer noopener"&gt;http://revistas.unanleon.edu.ni/index.php/REBICAMCLI/article/view/251&lt;/a&gt;</text>
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        <src>https://www.socictopen.socict.org/files/original/f95e25348009686d905593fc3733f003.pdf</src>
        <authentication>d7cb197c6a566791a4fef25fe8682991</authentication>
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          <name>Dublin Core</name>
          <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
          <elementContainer>
            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
              <elementTextContainer>
                <elementText elementTextId="88121">
                  <text>Agricultura sostenible</text>
                </elementText>
              </elementTextContainer>
            </element>
            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="88122">
                  <text>Dominio científico: Agricultura sostenible</text>
                </elementText>
              </elementTextContainer>
            </element>
          </elementContainer>
        </elementSet>
      </elementSetContainer>
    </collection>
    <itemType itemTypeId="1">
      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
    </itemType>
    <elementSetContainer>
      <elementSet elementSetId="1">
        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
        <elementContainer>
          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
            <elementTextContainer>
              <elementText elementTextId="187054">
                <text>La recuperación de pasivos ambientales desde el proyecto de paisaje</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="187055">
                <text>Karina Jensen, Mariana Birche</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="187056">
                <text>El presente trabajo se enmarca en el proyecto de investigación y transferencia en áreas prioritarias Parador Ecológico en Brandsen (2012-2014) que se ha desarrollado como una experiencia interdisciplinaria entre la Facultad de Ciencias Económicas y la Facultad de Arquitectura y Urbanismo (UNLP). El trabajo plantea incorporar el concepto de paisaje, sustentabilidad y medio ambiente al marco teórico del proyecto, con el objetivo de superar las ideas restringidas de ambiente y ecología, conformando un proyecto integral de paisaje. La metodología proyectual contempla el diagnóstico para el predio, afectado por las actividades extractivas, el programa de necesidades, el diseño de las edificaciones y talleres participativos. Se busca poner el acento en acciones tendientes a la remediación del pasivo ambiental, conformando un nuevo espacio de encuentro para la comunidad que contenga actividades recreativas, de carácter turístico y educación ambiental.</text>
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          <element elementId="40">
            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="187057">
                <text>2018</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="187058">
                <text>Ambiente, Cavas, Paisaje, Remediación, pasivo ambiental</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="43">
            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
            <elementTextContainer>
              <elementText elementTextId="187059">
                <text>10.24215/2346898Xe044</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="187060">
                <text>Geograficando</text>
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            </elementTextContainer>
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          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="187061">
                <text>Universidad Nacional de La Plata</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="38">
            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
            <elementTextContainer>
              <elementText elementTextId="187062">
                <text>Geography (General)</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="46">
            <name>Relation</name>
            <description>A related resource</description>
            <elementTextContainer>
              <elementText elementTextId="187063">
                <text>&lt;a href="https://www.geograficando.fahce.unlp.edu.ar/article/view/9349" target="_blank" rel="noreferrer noopener"&gt;https://www.geograficando.fahce.unlp.edu.ar/article/view/9349&lt;/a&gt;</text>
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            </elementTextContainer>
          </element>
        </elementContainer>
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