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                <text>Kuldeep Dhama, Bhoj R. Singh, Dharmender Kumar, Dharmendra K. Sinha, R. Karthikeyan, Shiv Varan Singh, Akanksha Yadav, Vinodh Kumar O.R., Richa Gandharva, Varsha Jayakumar, Sumedha Gandharava</text>
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                <text>This study analyzed the determinants of morbidity, mortality, and case fatality rate (CFR) of the ongoingpandemic of severe acute respiratory syndrome coronavirus-2 disease 2019 (COVID-19). Data for 210countries and territories available in public domains were analyzed in relation to mandatory vaccinationwith Bacille-Calmette-Guerin (BCG), population density, median age of the country population, healthcare expenditure per capita, life expectancy at birth, healthy life expectancy, literacy rate, per capitagross domestic production adjusted to purchasing power (PPP), burden of tuberculosis (TB), acquiredimmunodeficiency disease caused by human immunodeficiency virus (HIV-AIDS), malaria, cardiovasculardisease (CVD), neoplasm, diabetes, deaths due to energy-protein (food) deficiency (EPD), and percapita government spending on safe water and sanitation. Mandatory BCG vaccination showed ahighly significant (p</text>
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                <text>Kuldeep Dhama, Bhoj Raj Singh, Shiv Varan Singh, Karthikeyan Ravichandran, Subbaiyan Anbazhagan, Himani Agri, Ramkumar N. Rupner, Vinodh Kumar Obli Rajendran</text>
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                <text>Since the beginning of the New Year 2020, countries around the world are stumbling due to thecoronavirus disease (COVID-19) pandemic. Better approaches of diagnostics and medical facilitieshave helped some countries recover early. Previous exposures to epidemics have imparted lessons tohandle such a pandemic with a high level of preparedness. The World Health Organization (WHO) andnational health authorities are taking great efforts via efficient and impactful interventions to containthe virus. Diagnostic tests such as reverse transcription-polymerase chain reaction are increasinglybeing used to confirm the diagnosis because testing biological samples for the presence of the virusis the definitive method to identify the disease, analyze the risk for transmission, and determinewhether someone has been cured or not. It is also important to screen asymptomatic individualsto get the exact overview of the virus spread. Antibody detection plays a pivotal role in diagnosis;however, using it at the wrong time yields negative results and conveys dissenting opinion about thetests. Although the scaling up of testing has been significant, overall testing has been limited by theavailability of diagnostics. Rapid diagnoses and discontinuation of transmission are keys to endingthis pandemic. Diagnostics manufacturers are developing test kits and distributing them to differentcountries. Therefore, more than 500 commercial test kits for molecular- and immunoassays, most withEmergency Use Authorization, are now becoming available in the market. In this review, we discussthe importance of diagnostics, approaches of different countries toward the epidemic, global testingsituation, and lessons to countries at the start of the epidemic for better preparedness.</text>
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                <text>DOI: 10.22207/JPAM.14.SPL1.25</text>
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                <text>DOI: 10.1371/journal.pmed.0030001</text>
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                <text>Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus – 2(SARS-CoV-2), was declared a global pandemic on 11th March, 2020 by World Health Organization.As of now,27th May,2020, there are about 54,88,825 infected cases and 3,49,095 deaths globally.Coronavirus samples collected from all the countries have been sequenced for advanced studies ina bid to understand the structure and functioning of the virus. In our study we have tried workingon every available sequence to setup both comparisons and co-relations. There is no such availablestudy as of now for reference and hence it can become a pioneer stone in this direction. The mortalityrate calculated turns out to be 9.19%,34.37% and 6.29% for SARS-2003, MERS-2012 and COVID-19respectively. The basic reproduction rate R0 was 2-5 for SARS-2003, 0.3-0.8 for MERS-2012 and 1.4-5.7 for COVID-19. We found out the relation between number of mutations and mortality as well asphylogenetic relations. High number of mutations corresponded to higher mortality rate as in countrieslike Italy and Spain. Alpha and Beta-coronaviruses show a common ancestor from which they descended.Brazil and Iran have shown similar phylogenetic descent explaining their mortality rate. India howevershowed a distant relation from the common ancestor of other genome sequences. This study highlightsthe mutations of the SARS-CoV2 virus as well as sets up a comparison with the previous outbreaks.Similar type of studies should be conducted when more genome samples are present. These resultscan also contribute towards making an effective anti-viral therapy and vaccines.</text>
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                <text>Several viruses cause pulmonary infections due to their shared tropism with cells of the respiratory tract. These respiratory problems due to viral infection become a public health concern due to rapid transmission through air/aerosols or via direct-indirect contact with infected persons. In addition, the cross-species transmission causes alterations to viral genetic makeup thereby increasing the risk of emergence of pathogens with new and more potent infectivity. With the introduction of effective nucleic acid-based technologies, post translational gene silencing (PTGS) is being increasingly used to silence viral gene targets and has shown promising approach towards management of many viral infections. Since several host factors are also utilized by these viruses during various stages of infection, silencing these host factors can also serve as promising therapeutic tool. Several nucleic acid-based technologies such as short interfering RNAs (siRNA), antisense oligonucleotides, aptamers, deoxyribozymes (DNAzymes), and ribozymes have been studied and used against management of respiratory viruses. These therapeutic nucleic acids can be efficiently delivered through the airways. Studies have also shown efficacy of gene therapy in clinical trials against respiratory syncytial virus (RSV) as well as models of respiratory diseases including severe acute respiratory syndrome (SARS), measles and influenza. In this review, we have summarized some of the recent advancements made in the area of nucleic acid based therapeutics and highlighted the emerging roles of nucleic acids in the management of some of the severe respiratory viral infections. We have also focused on the methods of their delivery and associated challenges.</text>
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                <text>Kunling Shen, Spencer Li, Yu Guan, Yonghong Yang</text>
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                <text>10.1002/ped4.12229</text>
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