1613 10 20655 https://www.socictopen.socict.org/files/original/968abebab123b764e8ca8bd74ceec369.pdf c2ee015fb634fff3fd5489693d904a1e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Range structure, microhabitat use, and activity patterns of the saxicolous lizard Tropidurus torquatus (Tropiduridae) on a rock outcrop in Minas Gerais, Brazil Organización espacial, utilización de los microhábitats y padrones de actividad del lagarto saxícola Tropidurus torquatus (Tropiduridae) en un afloramiento rocoso en Minas Gerais, Brasil Creator An entity primarily responsible for making the resource LEONARDO B RIBEIRO, BERNADETE M SOUSA, SAMUEL C GOMIDES Description An account of the resource Although Tropidurus is a widely distributed lizard genus in South America and the Galapagos Islands, studies on space use and spatial distribution are scarce. We studied the home range structure of the saxicolous lizard Tropidurus torquatus based on the inland population of a rock outcrop in Minas Gerais State, southeastern Brazil. Lizards were individually marked and observed during reproductive and non-reproductive seasons. Using the mínimum convex polygon method, we found that average total range size of males during the reproductive season was larger than that of females, and that both had similar total range sizes in the non-reproductive season. The harmonic mean method showed that males have a larger home range size than that of females during both seasons. As expected for a polygynous species, the average number of males whose total ranges overlapped those of females tended to be higher in the reproductive season than in the non-reproductive season. Intrasexually, the number of females whose total ranges were associated with those of other females was also higher in the reproductive season than in the non-reproductive season. For males, this number remained low in both seasons, suggesting that males use more exclusive areas, whereas the smaller total ranges of females apparently sustain a higher density of individual s during the reproductive season. Frequency of microhabitat use in relation to vegetation increased in the non-reproductive season and the activity patterns of lizards shifted from bimodal in the reproductive season (rainy period) to unimodal in the non-reproductive season (dry period). Thus, the range structure, microhabitat use, and activity patterns of the T. torquatus observed here were all influenced by the time frame affecting their spatial ecology.Aunque Tropidurus es un género de lagarto extensamente distribuido en Sudamérica y en las islas Galápagos, son escasos los estudios sobre uso del espacio y distribución espacial. En este trabajo se estudió la organización espacial del lagarto saxícola Tropidurus torquatus basado en la población interiorana de uno afloramiento rocoso en el estado de Minas Gerais, sudeste del Brasil. Los lagartos fueron individualmente marcados y observados durante las estaciones reproductiva y no reproductiva. Con el método del mínimo polígono convexo fue encontrado que el tamaño promedio del dominio vital de los machos durante la estación reproductiva fue más grande que el de las hembras y en la estación no reproductiva hembras y machos mantuvieron dominios vitales similares en el tamaño. El método de la media armónica mostró que el tamaño promedio del area de vida de machos fue mayor que el area de las hembras en ambas estaciones. Como esperado para una especie poligínica, el número medio de machos con dominios vitales sobrepuestos a los de las hembras tendió a ser más grande en la estación reproductiva. Intrasexualmente, el número de hembras con sus dominios vitales asociados a los de otras hembras también fue mayor en la estación reproductiva. Para los machos, este número permaneció bajo en ambas estaciones, lo que sugiere que los machos usan areas más exclusivas, mientras los dominios vitales más pequeños de las hembras al parecer sostengan una mayor densidad de individuos durante la estación reproductiva. La frecuencia de uso de los microhábitats relacionados a la vegetación aumentó en la estación no reproductiva y el padrón de actividad de los lagartos cambió del bimodal en la estación reproductiva (período lluvioso) para unimodal en la no reproductiva (período seco). Así la organización espacial, la utilización de los microhábitats y los padrones de actividad de T. torquatus aquí observados fueron todos influenciados por el período de tiempo afectando la ecología espacial de los lagartos. Date A point or period of time associated with an event in the lifecycle of the resource 2009 Subject The topic of the resource Activity times, Home range, Tropidurus, dominio vital, habitat use, horarios de actividad, total range, utilización del habitat, área de vida Source A related resource from which the described resource is derived Revista Chilena de Historia Natural Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Botany, Zoology Relation A related resource <a href="http://www.scielo.cl/scielo.php?script=sci_arttext&amp;pid=S0716-078X2009000400011" target="_blank" rel="noreferrer noopener">http://www.scielo.cl/scielo.php?script=sci_arttext&amp;pid=S0716-078X2009000400011</a> https://www.socictopen.socict.org/files/original/b6afe0f66388456816c52a0b3acb944e.pdf 28ae0bcd067f5dbe3f48ff273ed611f2 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rango altitudinal: factor de vigor forestal y determinante en la regeneración natural del oyamel Creator An entity primarily responsible for making the resource Dante Arturo Rodríguez-Trejo, Alejandro Ismael Monterroso Rivas, Luis Felipe Romahn-Hernández, Antonio Villanueva-Morales, María de Jesús Pérez-Hernández Description An account of the resource Objetivo: evaluar el vigor del arbolado y la regeneración natural de oyamel al oriente del Estado de México, considerando variables topográficas y forestales. Método: se usó muestreo sistemático, con sitios cada 100 m s. n. m. entre los 2900 y 3600 m s. n. m. Con el programa sas se hicieron pruebas de normalidad, tablas de frecuencia y análisis de varianza para analizar la relación entre altitud, vigor y regeneración de Abies religiosa (Kunth) Schltdl. et Cham. Resultados: hay relación entre la altitud, el vigor y la densidad arbórea. La regeneración es nula en las cotas más bajas e incrementa con la altitud hasta los 3 400 m s. n. m. y no muestra gran reducción más arriba. La densidad de los estratos A (dap ≥12.5 cm) y B (dap 1 m de altura) incrementa conforme a la altitud; las densidades más altas se registran en la cota 3 400 m s. n. m. Limitaciones: se requiere investigación de otros factores que intervienen en la distribución de la especie. Principales hallazgos: la regeneración tiene más éxito en zonas medias y altas, y la condición del arbolado tiende a ser mejor ahí también. Se evidencia que el oyamel migra a zonas más elevadas, debido al cambio climático global. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource Abies religiosa, Regeneración natural, calentamiento global, declinación forestal, rango altitudinal Identifier An unambiguous reference to the resource within a given context 10.22201/enesl.20078064e.2020.22.72751e22.72751 Source A related resource from which the described resource is derived Entreciencias: Diálogos en la Sociedad del Conocimiento Publisher An entity responsible for making the resource available Universidad Nacional Autónoma de México Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Social Sciences, Science Relation A related resource <a href="http://www.redalyc.org/articulo.oa?id=457662386014" target="_blank" rel="noreferrer noopener">http://www.redalyc.org/articulo.oa?id=457662386014</a> https://www.socictopen.socict.org/files/original/3f0539e2bfe4c91a9b85b5f65dfa95d6.pdf d70e18e7ef83fe35e047d1df4110cf3c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapatriements en situation d'urgence lors de la pandémie de COVID-19: la solidarité européenne hors sol européen Creator An entity primarily responsible for making the resource Anastasia Iliopoulou-Penot Description An account of the resource (Series Information) European Papers - A Journal on Law and Integration, 2020 5(1), 469-477 | European Forum Insight of 16 May 2020 | (Table of Contents) I. Introduction. - II. Le droit à une protection consulaire des citoyens européens non représentés. - III. Le dispositif européen relatif au rapatriement en situation d'urgence. - IV. Enseignements et perspectives. | (Abstract) One of the priorities of the EU's external action during the COVID-19 outbreak was to ensure protection of its citizens stranded outside the European territory, especially those whose Member State of nationality had no embassy or consulate in a position to effectively provide consular assistance. Repatriation of these citizens was made possible because of the exercise of a right attached to EU citizenship and because of a European mechanism coordinating Member State action and EU support in the field of consular protection, especially in emergency situations. Some interesting lessons can be drawn from this experience concerning the extraterritorial dimension of EU citizenship and the role of the EU as a global actor. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource solidarity, covid-19 and the eu, repatriation, consular protection, unrepresented citizens, extraterritorial dimension of eu citizenship Identifier An unambiguous reference to the resource within a given context 10.15166/2499-8249/356 Source A related resource from which the described resource is derived Biotemas Publisher An entity responsible for making the resource available Universidade Federal de Santa Catarina Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Law, Law of Europe https://www.socictopen.socict.org/files/original/3c7623642cbc49e4eb071a7fe4a606d2.pdf d58d400d1f13832224ecd482db52a285 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis. Creator An entity primarily responsible for making the resource Mohd Azhar, Rhythm Phutela, Manoj Kumar, Asgar Hussain Ansari, Riya Rauthan, Sneha Gulati, Namrata Sharma, Dipanjali Sinha, Saumya Sharma, Sunaina Singh, Sundaram Acharya, Sajal Sarkar, Deepanjan Paul, Poorti Kathpalia, Meghali Aich, Paras Sehgal, Gyan Ranjan, Rahul C Bhoyar, Khushboo Singhal, Harsha Lad, Pradeep Kumar Patra, Govind Makharia, Giriraj Ratan Chandak, Bala Pesala, Debojyoti Chakraborty, Souvik Maiti Description An account of the resource Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR or sequencing are tough to implement with limited resources, necessitating the development of accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout for detecting nucleobase and nucleotide sequences without trans-cleavage of reporter molecules. We also demonstrate that FELUDA is 100% accurate in detecting single nucleotide variants (SNVs), including heterozygous carriers, and present a simple web-tool JATAYU to aid end-users. FELUDA is semi-quantitative, can adapt to multiple signal detection platforms, and deploy for versatile applications such as molecular diagnosis during infectious disease outbreaks like COVID-19. Employing a lateral flow readout, FELUDA shows 100% sensitivity and 97% specificity across all ranges of viral loads in clinical samples within 1hr. In combination with RT-RPA and a smartphone application True Outcome Predicted via Strip Evaluation (TOPSE), we present a prototype for FELUDA for CoV-2 detection closer to home. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource SARS-CoV-2, LFA, fncas 9, CRISPR Dx, FELUDA, SNV detection Identifier An unambiguous reference to the resource within a given context 10.1016/j.bios.2021.113207 Source A related resource from which the described resource is derived Biosensors & bioelectronics https://www.socictopen.socict.org/files/original/1988bd057b50ecc560c55a179b06710e.pdf 6b57f886325b85d6dd5a0df53f1768ea Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification Creator An entity primarily responsible for making the resource Jian-chang WANG, Jinfeng Wang, Ruiwen Li, Libing Liu, Wanzhe Yuan Description An account of the resource Abstract Background Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Recombinase polymerase amplification (RPA), as an isothermal gene amplification technique, has been explored for the molecular detection of diverse pathogens. Methods A real-time reverse transcription RPA (RT-RPA) assay for the detection of canine distemper virus (CDV) using primers and exo probe targeting the CDV nucleocapsid protein gene was developed. A series of other viruses were tested by the RT-RPA.Thirty-two field samples were further tested by RT-RPA, and the resuts were compared with those obtained by the real-time RT-PCR. Results The RT-RPA assay was performed successfully at 40 °C, and the results were obtained within 3 min–12 min. The assay could detect CDV, but did not show cross-detection of canine parvovirus-2 (CPV-2), canine coronavirus (CCoV), canine parainfluenza virus (CPIV), pseudorabies virus (PRV) or Newcastle disease virus (NDV), demonstrating high specificity. The analytical sensitivity of RT-RPA was 31.8 copies in vitro transcribed CDV RNA, which is 10 times lower than the real-time RT-PCR. The assay performance was validated by testing 32 field samples and compared to real-time RT-PCR. The results indicated an excellent correlation between RT-RPA and a reference real-time RT-PCR method. Both assays provided the same results, and R2 value of the positive results was 0.947. Conclusions The results demonstrated that the RT-RPA assay offers an alternative tool for simple, rapid, and reliable detection of CDV both in the laboratory and point-of-care facility, especially in the resource-limited settings. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource Canine Distemper Virus, nucleocapsid protein gene, Exo probe, Recombinase polymerase amplification, RPA and CDV Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12917-017-1180-7 Source A related resource from which the described resource is derived BMC Veterinary Research Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Veterinary medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/df56db4dcba4fc8d055e8b82882482c4.pdf 3cec953b714e1473b1d829c7f34beb8a Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick Creator An entity primarily responsible for making the resource Xiang Gao, Yong-lu Wang, Xin-sheng Liu, Yong-guang Zhang, Yanming Wei Description An account of the resource Abstract Background Porcine Deltacoronavirus (PDCoV) is a newly emerging Coronavirus that was first identified in 2012 in Hong Kong, China. Since then, PDCoV has subsequently been reported worldwide, causing a high number of neonatal piglet deaths and significant economic losses to the swine industry. Therefore, it is necessary to establish a highly sensitive and specific method for the rapid diagnosis of PDCoV. Results In the present study, a highly sensitive and specific diagnostic method using recombinase polymerase amplification combined with a lateral flow dipstick (LFD-RPA) was developed for rapid and visual detection of PDCoV. The system can be performed under a broad range of temperature conditions from 10 to 37 °C, and the detection of PDCoV can be completed in 10 min at 37 °C. The sensitivity of this assay was 10 times higher than that of conventional PCR with a lower detection limit of 1 × 102 copies/µl of PDCoV. Meanwhile, the LFD-RPA assay specifically amplified PDCoV, while there was no cross-amplification with other swine-associated viruses, including Porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), Porcine kobuvirus (PKoV), Foot and mouth disease virus (FMDV), Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine circovirus type 2 (PCV2), Classical swine fever virus (CSFV) and Seneca valley virus (SVV). The repeatability of the test results indicated that this assay had good repeatability. In addition, 68 clinical samples (48 fecal swab specimens and 20 intestinal specimens) were further tested by LFD-RPA and RT-PCR assay. The positive rate of LFD-RPA clinical samples was 26.47% higher than that of conventional PCR (23.53%). Conclusions The LFD-RPA assay successfully detected PDCoV in less than 20 min in this study, providing a potentially valuable tool to improve molecular detection for PDCoV and to monitor the outbreak of PDCoV, especially in low-resource areas and laboratories. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource lateral flow dipstick, rapid diagnosis, visual detection, Recombinase polymerase amplification, porcine deltacoronavirus Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12917-020-02341-3 Source A related resource from which the described resource is derived BMC Veterinary Research Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Veterinary medicine https://www.socictopen.socict.org/files/original/923a406bd2a53be5488e924a30c5e139.pdf 41a8b49b166503e6ade993ab4b8c7ff5 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid Antibody-based Tests for the Diagnosis of COVID-19: A Bigger Epidemic of Unscientific Practices Creator An entity primarily responsible for making the resource Sarman Singh Date A point or period of time associated with an event in the lifecycle of the resource 2020 Identifier An unambiguous reference to the resource within a given context DOI: 10.1055/s-0040-1712711 Source A related resource from which the described resource is derived Journal of Laboratory Physicians Publisher An entity responsible for making the resource available Wolters Kluwer Medknow Publications Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine https://www.socictopen.socict.org/files/original/a1459af6e4180399c69f253ae1acc649.pdf 7b15437af5e9c210ea3cdf5f9d6c9417 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid Antigen Detection Test for Severe Acute Respiratory Syndrome Coronavirus 2: How to Use It Properly? Creator An entity primarily responsible for making the resource Tonny Loho, Djoko Widodo Description An account of the resource Cases of coronavirus disease 2019 (COVID-19) in Indonesia are still increasing and even higher in the last few weeks. Contact tracing and surveillance are important to locate cases in the community, including asymptomatic individuals. Diagnosis of COVID-19 depends on the detection of viral RNA, viral antigen, or indirectly, viral antibodies. Molecular diagnosis, using real time, reverse transcriptase polymerase chain reaction (RT-PCR), is the common standard method; however, it is not widely available in Indonesia and requires a high standard laboratory. Rapid, point-of-care antibody testing has been widely used as an alternative; however, interpretation of the results is not simple and now it is no longer used by the Indonesian government as a screening test for people travelling between locations. Thus, the rapid antigen detection test (Ag-RDT) is used by the Indonesian government as a screening test for travellers. As a result, many people buy the kit online and perform self-Ag-RDT at home. This raises the question of how safe and accurate it is to perform self-Ag-RDT at home. Before a test is applied, it is suggested to research its sensitivity and specificity, as compared to gold standard, and its limitations. In this article, laboratory diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is discussed, with an emphasis on Ag-RDT and the recommendation to use it properly in daily practice. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource covid-19, SARS-CoV-2, Antigen testing Source A related resource from which the described resource is derived Acta Medica Indonesiana Publisher An entity responsible for making the resource available Interna Publishing Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Internal medicine https://www.socictopen.socict.org/files/original/76a6c52f5a5df7c45ab5e91d68142cad.pdf 9fa94c71c2c42a5b04eee8cf770fc0a3 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid community point-of-care testing for COVID-19 (RAPTOR-C19): protocol for a platform diagnostic study Creator An entity primarily responsible for making the resource Brian D. Nicholson, Philip J. Turner, Rafael Perera, Jason Oke, Gail Hayward, F. D. Richard Hobbs, Thomas Fanshawe, Abigail Moore, Filipa Ferreira, Simon de Lusignan, Julian Sherlock, Maria Zambon, Constantinos Koshiaris, Rachel Byford, Joseph J. Lee, Alexandra Deeks, Mary Logan, Anna Seeley, James P. Sheppard, Uy Hoang, Vaishnavi Parimalanathan, George Edwards, Harshana Liyange, Joanna Ellis, Jamie Lopez Bernal, Gayatri Amirthalingam, Ezra Linley, Ray Borrow, Gary Howsam, Sophie Baines Description An account of the resource Abstract Background The aim of RApid community Point-of-care Testing fOR COVID-19 (RAPTOR-C19) is to assess the diagnostic accuracy of multiple current and emerging point-of-care tests (POCTs) for active and past SARS-CoV2 infection in the community setting. RAPTOR-C19 will provide the community testbed to the COVID-19 National DiagnOstic Research and Evaluation Platform (CONDOR). Methods RAPTOR-C19 incorporates a series of prospective observational parallel diagnostic accuracy studies of SARS-CoV2 POCTs against laboratory and composite reference standards in patients with suspected current or past SARS-CoV2 infection attending community settings. Adults and children with suspected current SARS-CoV2 infection who are having an oropharyngeal/nasopharyngeal (OP/NP) swab for laboratory SARS-CoV2 reverse transcriptase Digital/Real-Time Polymerase Chain Reaction (d/rRT-PCR) as part of clinical care or community-based testing will be invited to participate. Adults (≥ 16 years) with suspected past symptomatic infection will also be recruited. Asymptomatic individuals will not be eligible. At the baseline visit, all participants will be asked to submit samples for at least one candidate point-of-care test (POCT) being evaluated (index test/s) as well as an OP/NP swab for laboratory SARS-CoV2 RT-PCR performed by Public Health England (PHE) (reference standard for current infection). Adults will also be asked for a blood sample for laboratory SARS-CoV-2 antibody testing by PHE (reference standard for past infection), where feasible adults will be invited to attend a second visit at 28 days for repeat antibody testing. Additional study data (e.g. demographics, symptoms, observations, household contacts) will be captured electronically. Sensitivity, specificity, positive, and negative predictive values for each POCT will be calculated with exact 95% confidence intervals when compared to the reference standard. POCTs will also be compared to composite reference standards constructed using paired antibody test results, patient reported outcomes, linked electronic health records for outcomes related to COVID-19 such as hospitalisation or death, and other test results. Discussion High-performing POCTs for community use could be transformational. Real-time results could lead to personal and public health impacts such as reducing onward household transmission of SARS-CoV2 infection, improving surveillance of health and social care staff, contributing to accurate prevalence estimates, and understanding of SARS-CoV2 transmission dynamics in the population. In contrast, poorly performing POCTs could have negative effects, so it is necessary to undertake community-based diagnostic accuracy evaluations before rolling these out. Trial registration ISRCTN, ISRCTN14226970 Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource covid-19, primary care, Diagnostic Tests, point-of-care tests, community testing Identifier An unambiguous reference to the resource within a given context 10.1186/s41512-021-00093-8 Source A related resource from which the described resource is derived Epidemiology and Health Publisher An entity responsible for making the resource available Korean Society of Epidemiology Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine (General) https://www.socictopen.socict.org/files/original/4278bc1799a8a58c41ee7a0061cce87b.pdf 4d5e70d0b59973564761acf33219a267 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Rapid decay of host basal mRNAs during SARS-CoV-2 infection perturbs host antiviral mRNA biogenesis and export. Creator An entity primarily responsible for making the resource James M Burke, Laura A St Clair, Rushika Perera, Roy Parker Description An account of the resource A key feature of the mammalian innate immune response to viral infection is the transcriptional induction of interferon (IFN) genes, which encode for secreted proteins that prime the antiviral response and limit viral replication and dissemination. A hallmark of severe COVID-19 disease caused by SARS-CoV-2 is the low presence of IFN proteins in patient serum despite elevated levels of IFN -encoding mRNAs, indicative of post-transcriptional inhibition of IFN protein production. Herein, we show SARS-CoV-2 infection limits type I and type III IFN biogenesis by preventing the release of mRNA from their sites of transcription and/or triggering their nuclear degradation. In addition, SARS-CoV-2 infection inhibits nuclear-cytoplasmic transport of IFN mRNAs as a consequence of widespread cytosolic mRNA degradation mediated by both activation of the host antiviral endoribonuclease, RNase L, and by the SARS-CoV-2 protein, Nsp1. These findings argue that inhibition of host and/or viral Nsp1-mediated mRNA decay, as well as IFN treatments, may reduce viral-associated pathogenesis by promoting the innate immune response. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Identifier An unambiguous reference to the resource within a given context 10.1101/2021.04.19.440452 Source A related resource from which the described resource is derived bioRxiv : the preprint server for biology