231 10 20655 https://www.socictopen.socict.org/files/original/0e11df12f35ed430adaaedc30e4ce98d.pdf 2ddfd3332002116c0ec265a35f7beea0 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Viral-bacterial co-infection in Australian Indigenous children with acute otitis media Creator An entity primarily responsible for making the resource Whiley David, Nissen Michael, Sloots Theo, Smith-Vaughan Heidi, Cheng Allen C, Binks Michael J, McDonnell Joseph, Leach Amanda J Description An account of the resource Abstract Background Acute otitis media with perforation (AOMwiP) affects 40% of remote Indigenous children during the first 18 months of life. Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis are the primary bacterial pathogens of otitis media and their loads predict clinical ear state. Our hypothesis is that antecedent respiratory viral infection increases bacterial density and progression to perforation. Methods A total of 366 nasopharyngeal swabs from 114 Indigenous children were retrospectively examined. A panel of 17 respiratory viruses was screened by PCR, and densities of S. pneumoniae, H. influenzae and M. catarrhalis were estimated by quantitative real time PCR. Data are reported by clinical ear state. Results M. catarrhalis (96%), H. influenzae (91%), S. pneumoniae (89%) and respiratory viruses (59%) were common; including rhinovirus (HRV) (38%), polyomavirus (HPyV) (14%), adenovirus (HAdV) (13%), bocavirus (HBoV) (8%) and coronavirus (HCoV) (4%). Geometric mean bacterial loads were significantly higher in children with acute otitis media (AOM) compared to children without evidence of otitis media. Children infected with HAdV were 3 times more likely (p < 0.001) to have AOM with or without perforation. Conclusion This study confirms a positive association between nasopharyngeal bacterial load and clinical ear state, exacerbated by respiratory viruses, in Indigenous children. HAdV was independently associated with acute ear states. Date A point or period of time associated with an event in the lifecycle of the resource 2011 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1471-2334-11-161 Source A related resource from which the described resource is derived BMC Infectious Diseases Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/09b526e1c575a9e3f0f239545acea8a0.pdf ab527c700951c60aad1bee1981bc83b8 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Jakob Lubbes Familienaufzeichnungen als Zeugnis der Lebensselbstdarstellung eines Krämers im Danzig um die Wende des 15. Jahrhunderts Creator An entity primarily responsible for making the resource Julia Możdżeń Description An account of the resource The family notes of Gdańsk stall-holder Jakob Lubbe were wrongly referred to as a family chronicle. The new critical analysis of the manuscript shows that notes copied by Lubbe’s descendant – a Dominican Martin Gruneweg – came originally from the trade book kept by Jacob. This fact put Jacob Lubbe’s notes in a new light. They constitute a kind of self-testimony which represent a stream of conscience on the world. The notes which were analysed concerned mainly the family and religiousness of Jakob. Lubbe’s notes allow us also to learn about his subjective perception of the social roles played by him – roles of a stepfather, husband, senior guild member, a member of a parish and the city community. Jacob’s notes show how family problems and professional issues interwove in the merchant house of this Gdańsk stall-holder. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource Prusy Królewskie, Gdańsk, XV wiek, kupcy, księga handlowa, rodzina Identifier An unambiguous reference to the resource within a given context DOI: 10.12775/BPMH.2013.006 Source A related resource from which the described resource is derived Biuletyn Polskiej Misji Historycznej Publisher An entity responsible for making the resource available Nicolaus Copernicus University in Toruń Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant History of Eastern Europe, History of Central Europe Language A language of the resource DE, EN https://www.socictopen.socict.org/files/original/c263ca0c4eea780302639292a39d9118.pdf 718d2bb52c01ffe2ed6795cffdf10855 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource How Do the First Days Count? A Case Study of Qatar Experience in Emergency Risk Communication during the MERS-CoV Outbreak Creator An entity primarily responsible for making the resource Mohamed Nour, Mohd AlHajri, Elmoubasher A. B. A. Farag, Hamad E. Al-Romaihi, Mohamed Al-Thani, Salih Al-Marri, Elena Savoia Description An account of the resource This case study is the first to be developed in the Middle East region to document what happened during the response to the 2013 MERS outbreak in Qatar. It provides a description of key epidemiologic events and news released from a prime daily newspaper and main Emergency Risk Communication (ERC) actions that were undertaken by public health authorities. Using the Crisis and Emergency Risk Communication (CERC) theoretical framework, the study analyzes how the performed ERC strategies during the first days of the outbreak might have contributed to the outbreak management. Methods: MERS-CoV related events were chronologically tracked, together with the relevant stories that were published in a major newspaper over the course of three distinct phases of the epidemic. The collected media stories were then assessed against the practiced emergency risk communication (ERC) activities during the same time frame. Results: The Crisis &amp; Emergency Risk Communication (CERC) framework was partially followed during the early days of the MERS-CoV epidemic, which were characterized by overwhelming uncertainty. The SCH’s commitment to a proactive and open risk communication strategy since day one, contributed to creating the SCH’s image as a credible source of information and allowed for the quick initiation of the overall response efforts. Yet, conflicting messages and over reassurance were among the observed pitfalls of the implemented ERC strategy. Conclusion: The adoption of CERC principles can help restore and maintain the credibility of responding agencies. Further work is needed to develop more rigorous and comprehensive research strategies that address sharing of information by mainstream as well as social media for a more accurate assessment of the impact of the ERC strategy. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource MERS, emergency risk communication, coordination, Qatar, CERC, Outbreak Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/ijerph14121597 Source A related resource from which the described resource is derived International Journal of Environmental Research and Public Health Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/a793b805cb5caaadf9dda1581e0317ca.pdf 49e6709c71abd8a4a63f70201564ea1d Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells Creator An entity primarily responsible for making the resource Chen Jiapei, Zhao Zhenhu, Shan Yajun, Yuan Xiaoling, Cong Yuwen Description An account of the resource Abstract Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), cause of the life-threatening atypical pneumonia, infects many organs, such as lung, liver and immune organ, and induces parenchyma cells apoptosis and necrosis. The genome of SARS-CoV, not closely related to any of the previously characterized coronavirus, encodes replicase and four major structural proteins and a number of non-structural proteins. Published studies suggest that some non-structural proteins may play important roles in the replication, virulence and pathogenesis of viruses. Among the potential SARS-CoV non-structural proteins, 3b protein (ORF4) is predicted encoding 154 amino acids, lacking significant similarities to any known proteins. Till now, there is no report about the function of 3b protein. In this study, 3b gene was linked with the EGFP tag at the C- terminus. Through cell cycle analysis, it was found that over-expression of 3b-EGFP protein in Vero, 293 and COS-7 cells could induce cell cycle arrest at G0/G1 phase, and that especially in COS-7 cells, expression of 3b-EGFP was able to induce the increase of sub-G1 phase from 24 h after transfection, which was most obvious at 48 h. The apoptosis induction of 3b fusion protein in COS-7 cells was further confirmed by double cell labeling with 7-AAD and Annexin V, the function of 3b protein inducing cell G0/G1 arrest and apoptosis may provide a new insight for further study on the mechanism of SARS pathogenesis. Date A point or period of time associated with an event in the lifecycle of the resource 2005 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1743-422X-2-66 Source A related resource from which the described resource is derived Virology Journal Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/a10425508191ae8d3f4d583025f4ce86.pdf b1b6f23c187472e5475fe2c5813e8347 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource MegaGTA: a sensitive and accurate metagenomic gene-targeted assembler using iterative de Bruijn graphs Creator An entity primarily responsible for making the resource Dinghua Li, Yukun Huang, Chi-Ming Leung, Ruibang Luo, Hing-Fung Ting, Tak-Wah Lam Description An account of the resource Abstract Background The recent release of the gene-targeted metagenomics assembler Xander has demonstrated that using the trained Hidden Markov Model (HMM) to guide the traversal of de Bruijn graph gives obvious advantage over other assembly methods. Xander, as a pilot study, indeed has a lot of room for improvement. Apart from its slow speed, Xander uses only 1 k-mer size for graph construction and whatever choice of k will compromise either sensitivity or accuracy. Xander uses a Bloom-filter representation of de Bruijn graph to achieve a lower memory footprint. Bloom filters bring in false positives, and it is not clear how this would impact the quality of assembly. Xander does not keep track of the multiplicity of k-mers, which would have been an effective way to differentiate between erroneous k-mers and correct k-mers. Results In this paper, we present a new gene-targeted assembler MegaGTA, which attempts to improve Xander in different aspects. Quality-wise, it utilizes iterative de Bruijn graphs to take full advantage of multiple k-mer sizes to make the best of both sensitivity and accuracy. Computation-wise, it employs succinct de Bruijn graphs (SdBG) to achieve low memory footprint and high speed (the latter is benefited from a highly efficient parallel algorithm for constructing SdBG). Unlike Bloom filters, an SdBG is an exact representation of a de Bruijn graph. It enables MegaGTA to avoid false-positive contigs and to easily incorporate the multiplicity of k-mers for building better HMM model. We have compared MegaGTA and Xander on an HMP-defined mock metagenomic dataset, and showed that MegaGTA excelled in both sensitivity and accuracy. On a large rhizosphere soil metagenomic sample (327Gbp), MegaGTA produced 9.7–19.3% more contigs than Xander, and these contigs were assigned to 10–25% more gene references. In our experiments, MegaGTA, depending on the number of k-mers used, is two to ten times faster than Xander. Conclusion MegaGTA improves on the algorithm of Xander and achieves higher sensitivity, accuracy and speed. Moreover, it is capable of assembling gene sequences from ultra-large metagenomic datasets. Its source code is freely available at https://github.com/HKU-BAL/megagta . Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource metagenomics, assembly, de Bruijn Graph, Targeted gene Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12859-017-1825-3 Source A related resource from which the described resource is derived BMC Bioinformatics Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Computer applications to medicine. Medical informatics Language A language of the resource EN https://www.socictopen.socict.org/files/original/dfe1a2bc76cceaebd3afddd94839ee46.pdf ed4b17d7a053faa8ce43d5cd3900837c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark Creator An entity primarily responsible for making the resource Christina M. Lazov, Mariann Chriél, Hans J. Baagøe, Esben Fjederholt, Yu Deng, Engbert A. Kooi, Graham J Belsham, Anette Bøtner, Thomas Bruun Rasmussen Description An account of the resource Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource Vespertilionidae, coronavirus, nucleotide sequencing, Phylogenetic analysis, Europe, host restriction Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v10090486 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN https://www.socictopen.socict.org/files/original/b8713a07a9aadf8f4ae7662e6861e0c6.pdf a3d80685057dc21766ee96afbdb52fc7 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource EFFECTIVENESS OF INTERNATIONAL HEALTH REGULATIONS IN PREVENTION AND CONTROL OF EMERGENCY SITUATIONS OF SANITARY-EPIDEMIOLOGICAL AND BIOLOGICAL CHARACTER Creator An entity primarily responsible for making the resource A.Yu. Popova, V.P. Toporkov, V.Yu. Smolensky, N. D. Pakskina, S. K. Udovichenko, I. G. Karnaukhov, A. E. Shiyanova, S.A. Shcherbakova, V. V. Kutyrev Description An account of the resource The paper describes the International Health Regulations (2005), hereinafter referred as IHR (2005), by the example of SARS, A/H5N1/, A/H1N1/09, Zika fever, and MERS-CoV control as an effective tool for prevention and control of public health emergences (ES of sanitary-epidemiological character) of international concern. Late detection of Ebola fever epidemic in West Africa (2013–2016) and announcement of public health emergency of international concern at the time when epidemic up-scaled to a threat to national security of the affected countries and high-priority threat to global community (ES of biological character) is attributed to non-use of methodological capacities of IHR (2005) on verification of ES at an early stage of epidemic development because of unpreparedness of local, regional, national and international public health services to such an epidemic situation. WHO plans to achieve scientifically-based reduction of the time from the onset of epidemic events to the effective response measures. Russian Federation, scientifically implementing IHR (2005) from the very beginning and having devised methodology of IHR implementation on the national level, has a strong premise for scientific provision of this process. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource IHR (2005), emergency situation of sanitary-epidemiological (biological) character of international concern, ebola fever epidemic in west africa, methodology of ihr (2005) implementation on the national level Identifier An unambiguous reference to the resource within a given context DOI: 10.21055/0370-1069-2017-2-5-8 Source A related resource from which the described resource is derived Проблемы особо опасных инфекций Publisher An entity responsible for making the resource available Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource RU https://www.socictopen.socict.org/files/original/b9336d25547aafef447e51dee5e45a01.pdf 8b380314fe75fb1ecdb536b4d1986e9a Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bioinformatics and evolutionary insight on the spike glycoprotein gene of QX-like and Massachusetts strains of infectious bronchitis virus Creator An entity primarily responsible for making the resource Abro Shahid, Ullman Karin, Belák Sándor, Baule Claudia Description An account of the resource Abstract Background Infectious bronchitis virus (IBV) is a Gammacoronavirus of the family Coronaviridae and is a causative agent of an economically important disease in poultry. The spike glycoprotein of IBV is essential for host cell attachment, neutralization, and is involved in the induction of protective immunity. Previously obtained sequence data of the spike gene of IBV QX-like and Massachusetts strains were subjected to bioinformatics analysis. Findings On analysis of potential phosphorylation sites, the Ser542 and Ser563 sites were not present in Massachusetts strains, while QX-like isolates did not have the Ser534 site. Massachusetts and QX-like strains showed different cleavage site motifs. The N-glycosylation sites ASN-XAA-SER/THR-55, 147, 200 and 545 were additionally present in QX-like strains. The leucine-rich repeat regions in Massachusetts strains consisted of stretches of 63 to 69 amino acids, while in the QX-like strains they contained 59 amino acids in length. An additional palmitoylation site was observed in CK/SWE/082066/2010 a QX-like strain. Primary structure data showed difference in the physical properties and hydrophobic nature of both genotypes. The comparison of secondary structures revealed no new structural domains in the genotypic variants. The phylogenetic analyses based on avian and mammalian coronaviruses showed the analysed IBV as closely related to turkey coronaviruses and distantly related to thrush and munia coronaviruses. Conclusion The study demonstrated that spike glycoprotein of the Massachusetts and the QX-like variants of IBV are molecularly distinct and that this may reflect in differences in the behavior of these viruses in vivo. Date A point or period of time associated with an event in the lifecycle of the resource 2012 Subject The topic of the resource infectious bronchitis virus, spike glycoprotein, Bioinformatics, coronavirus, evolution Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1743-422X-9-211 Source A related resource from which the described resource is derived Virology Journal Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/fca6ed92e81cc0aa13aa9bf6e4296ee1.pdf 684a629f95aae92391e587dc18735340 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A reverse transcription loop-mediated isothermal amplification (LAMP) assay for the detection of feline Coronavirus Creator An entity primarily responsible for making the resource Angelica Stranieri, Stefania Lauzi, Alessia Giordano, Saverio Paltrinieri Description An account of the resource Loop-mediated isothermal amplification (LAMP) is a molecular method that amplifies DNA under isothermal conditions. It relies on the use of 4 different primers recognizing 6 regions of the template sequence and on the use of a DNA polymerase with strand displacement activity (Notomi et al., 2000). The addition of two loop primers allows the reaction time to be of one hour only (Nagamine et al., 2002). The aim of this study was to develop a reverse transcription LAMP assay for an easy and inexpensive detection of feline Coronavirus (FCoV). Six primers binding the conserved 3’UTR region of the FCoV were designed with the Primer Explorer software. Thirty-two samples of RNA (11 feces, 8 effusions, 9 blood samples and 4 tissues) on which a reverse transcription polymerase chain reaction (RT-PCR) for the 3’UTR region was performed were used. The reaction was carried out in 25μL reaction volume and the mixture was incubated in a thermocycler at 63°C for 1 hour followed by 10 minutes at 80°C. LAMP products were visualized under UV after electrophoresis migration on a 1.5% agarose gel stained with ethidium bromide, where they produce a ladder-like pattern if positive. Results where compared with those obtained on standard PCR. Sensitivity and specificity were respectively 60% and 100% on feces, 40% and 100% on effusions, 25% and 100% on blood, and 100% and 100% on tissues. The overall sensitivity and specificity of this method were of 57.1% and 100%, thus limiting a clinical application of this method, except for tissues. Date A point or period of time associated with an event in the lifecycle of the resource 2016 Subject The topic of the resource LAMP, Feline coronavirus, PCR Identifier An unambiguous reference to the resource within a given context DOI: 10.13130/2283-3927/7075 Source A related resource from which the described resource is derived International Journal of Health, Animal Science and Food Safety Publisher An entity responsible for making the resource available Università degli Studi di Milano Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Genetics, Ecology, Animal culture, Economic biology Language A language of the resource EN https://www.socictopen.socict.org/files/original/4b331d28bbc72dbcfd9f77385048755b.pdf f0c06e59d90b5559ebc1e48bd87adb84 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Identification of a Novel Inhibitor against Middle East Respiratory Syndrome Coronavirus Creator An entity primarily responsible for making the resource Ya-Ping Sun, Huaidong Zhang, Jian Shi, Zhe Zhang, Rui Gong Description An account of the resource The Middle East respiratory syndrome coronavirus (MERS-CoV) was first isolated in 2012, and circulated worldwide with high mortality. The continual outbreaks of MERS-CoV highlight the importance of developing antiviral therapeutics. Here, we rationally designed a novel fusion inhibitor named MERS-five-helix bundle (MERS-5HB) derived from the six-helix bundle (MERS-6HB) which was formed by the process of membrane fusion. MERS-5HB consists of three copies of heptad repeat 1 (HR1) and two copies of heptad repeat 2 (HR2) while MERS-6HB includes three copies each of HR1 and HR2. As it lacks one HR2, MERS-5HB was expected to interact with viral HR2 to interrupt the fusion step. What we found was that MERS-5HB could bind to HR2P, a peptide derived from HR2, with a strong affinity value (KD) of up to 0.24 nM. Subsequent assays indicated that MERS-5HB could inhibit pseudotyped MERS-CoV entry effectively with 50% inhibitory concentration (IC50) of about 1 μM. In addition, MERS-5HB significantly inhibited spike (S) glycoprotein-mediated syncytial formation in a dose-dependent manner. Further biophysical characterization showed that MERS-5HB was a thermo-stable α-helical secondary structure. The inhibitory potency of MERS-5HB may provide an attractive basis for identification of a novel inhibitor against MERS-CoV, as a potential antiviral agent. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource MERS-CoV, fusion inhibitor, six-helix bundle, heptad repeat, five-helix bundle Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v9090255 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN