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                <text>A checklist to improve health system resilience to infectious disease outbreaks and natural hazards</text>
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                <text>David Bishai, Jennifer B Nuzzo, Diane Meyer, Sanjana J Ravi, Harunor Rashid, Shehrin Shaila Mahmood, Eric Toner</text>
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                <text>Recent infectious disease outbreaks, including the ongoing global COVID-19 pandemic and Ebola in the Democratic Republic of the Congo, have demonstrated the critical importance of resilient health systems in safeguarding global health security. Importantly, the human, economic and political tolls of these crises are being amplified by health systems’ inabilities to respond quickly and effectively. Improving resilience within health systems can build on pre-existing strengths to enhance the readiness of health system actors to respond to crises, while also maintaining core functions. Using data gathered from a scoping literature review, interviews with key informants and from stakeholders who attended a workshop held in Dhaka, Bangladesh, we developed a Health System Resilience Checklist (‘the checklist’). The aim of the checklist is to measure the specific capacities, capabilities and processes that health systems need in order to ensure resilience in the face of both infectious disease outbreaks and natural hazards. The checklist is intended to be adapted and used in a broad set of countries as a component of ongoing processes to ensure that health actors, institutions and populations can mount an effective response to infectious disease outbreaks and natural hazards while also maintaining core healthcare services. The checklist is an important first step in improving health system resilience to these threats, but additional research and resources will be necessary to further refine and prioritise the checklist items and to pilot the checklist with the frontline health facilities that would be using it. This will help ensure its feasibility and durability for the long-term within the health systems strengthening and health security fields.</text>
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                <text>Epidemiology and Health</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>Infectious and parasitic diseases, Medicine (General)</text>
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                <text>A child with household transmitted COVID-19</text>
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                <text>Jian Xu, Dan Zhang, Peng Zhao, Bin Li, Jian-Wei Pan, Zhihao Xu, Xiao-Ping Xia, Yuehong Wang, Jian-Guo He, Yue Su, Lijuan Mao, Zhe-Feng YUAN</text>
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                <text>Abstract Background Although people of all ages are susceptible to the novel coronavirus infection, which is presently named “Coronavirus Disease 2019” (COVID-19), there has been relatively few cases reported among children. Therefore, it is necessary to understand the clinical characteristics of COVID-19 in children and the differences from adults. Case presentation We report one pediatric case of COVID-19. A 14-month-old boy was admitted to the hospital with a symptom of fever, and was diagnosed with a mild form of COVID-19. The child’s mother and grandmother also tested positive for SARS-CoV-2 RNA. However, the lymphocyte counts were normal. The chest computed tomography (CT) revealed scattered ground glass opacities in the right lower lobe close to the pleura and resorption after the treatment. The patient continued to test positive for SARS-CoV-2 RNA in the nasopharyngeal swabs and stool at 17 days after the disappearance of symptoms. Conclusion The present pediatric case of COVID-19 was acquired through household transmission, and the symptoms were mild. Lymphocyte counts did not significantly decrease. The RNA of SARS-CoV-2 in stool and nasopharyngeal swabs remained positive for an extended period of time after the disappearance of symptoms. This suggests that attention should be given to the potential contagiousness of pediatric COVID-19 cases after clinical recovery.</text>
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                <text>DOI: 10.1186/s12879-020-05056-w</text>
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                <text>BMC Infectious Diseases</text>
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                <text>Infectious and parasitic diseases</text>
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                <text>Li-juan Mao, Jian Xu, Zhi-hao Xu, Xiao-ping Xia, Bin Li, Jian-guo He, Peng Zhao, Jian-wei Pan, Dan Zhang, Yue Su, Yue-hong Wang, Zhe-feng Yuan</text>
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                <text>Abstract Background Although people of all ages are susceptible to the novel coronavirus infection, which is presently named “Coronavirus Disease 2019” (COVID-19), there has been relatively few cases reported among children. Therefore, it is necessary to understand the clinical characteristics of COVID-19 in children and the differences from adults. Case presentation We report one pediatric case of COVID-19. A 14-month-old boy was admitted to the hospital with a symptom of fever, and was diagnosed with a mild form of COVID-19. The child’s mother and grandmother also tested positive for SARS-CoV-2 RNA. However, the lymphocyte counts were normal. The chest computed tomography (CT) revealed scattered ground glass opacities in the right lower lobe close to the pleura and resorption after the treatment. The patient continued to test positive for SARS-CoV-2 RNA in the nasopharyngeal swabs and stool at 17 days after the disappearance of symptoms. Conclusion The present pediatric case of COVID-19 was acquired through household transmission, and the symptoms were mild. Lymphocyte counts did not significantly decrease. The RNA of SARS-CoV-2 in stool and nasopharyngeal swabs remained positive for an extended period of time after the disappearance of symptoms. This suggests that attention should be given to the potential contagiousness of pediatric COVID-19 cases after clinical recovery.</text>
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                <text>Chromatin insulators, such as the chicken β-globin locus control region hypersensitive site 4 (HS4), and scaffold/matrix attachment regions (SARs/MARs) have been incorporated separately or in combination into retroviral vectors (RVs) in order to increase transgene expression levels, avoid silencing and reduce expression variability. However, their incorporation into RVs either produces a reduction on titer and/or expression levels or do not have sufficient effect on stem cells. In order to develop an improved insulator we decided to combine SAR elements with HS4 insulators. We designed several synthetic shorter SAR elements containing 4 or 5 MAR/SARs recognition signatures (MRS) and studied their effects on a lentiviral vector (LV) expressing eGFP through the SFFV promoter (SE). A 388 bp SAR element containing 5 MRS, named SAR2, was as efficient or superior to the other SARs analyzed. SAR2 enhanced transgene expression and reduced silencing and variability on human embryonic stem cells (hESCs). We next compared the effect of different HS4-based insulators, the HS4-Core (250 bp), the HS4-Ext (400 bp) and the HS4-650 (650 bp). All HS4 elements reduced silencing and expression variability but they also had a negative effect on transgene expression levels and titer. In general, the HS4-650 element had a better overall effect. Based on these data we developed a chimeric insulator, IS2, combining the SAR2 and the HS4-650. When incorporated into the 3' LTR of the SE LV, the IS2 element was able to enhance expression, avoid silencing and reduce variability of expression on hESCs. Importantly, these effects were maintained after differentiation of the transduced hESCs toward the hematopoietic linage. Neither the HS4-650 nor the SAR2 elements had these effects. The IS2 element is therefore a novel insulator that confers expression stability and enhances expression of LVs on stem cells.</text>
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                <text>DOI: 10.1371/journal.pone.0084268</text>
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                <text>PLoS ONE</text>
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                <text>A Chinese Case of Coronavirus Disease 2019 (COVID-19) Did Not Show Infectivity During the Incubation Period: Based on an Epidemiological Survey</text>
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                <text>Jong-Myon Bae</text>
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                <text>Controversy remains over whether the coronavirus disease 2019 (COVID-19) virus may have infectivity during the incubation period before the onset of symptoms. The author had the opportunity to examine the infectivity of COVID-19 during the incubation period by conducting an epidemiological survey on a confirmed patient who had visited Jeju Island during the incubation period. The epidemiological findings support the claim that the COVID-19 virus does not have infectivity during the incubation period.</text>
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                <text>2020</text>
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                <text>COVID-19, coronavirus, infectious disease incubation period, Epidemiology</text>
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                <text>DOI: 10.3961/jpmph.20.048</text>
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            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="2405">
                <text>Journal of Preventive Medicine and Public Health</text>
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            <name>Publisher</name>
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                <text>Korean Society for Preventive Medicine</text>
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                <text>Public aspects of medicine, Medicine</text>
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                  <text>Agricultura sostenible</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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            <name>Title</name>
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                <text>A cidadania no meio rural: uma análise do Programa Nacional de Fortalecimento da Agricultura Familiar a partir da Teoria do Discurso</text>
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            <description>An entity primarily responsible for making the resource</description>
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                <text>Anna Cláudia  Campos e Santos, Rennan Lanna Martins  Mafra</text>
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            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Tomando o Programa Nacional de Fortalecimento da Agricultura Familiar (Pronaf) como a principal resposta dada pelo Estado à institucionalização da cidadania no meio rural pós-Constituição de 1988, este trabalho busca por meio da divisão histórica demarcada de 1995-2002, realizar uma ressignificação histórica do desenvolvimento de tal programa, a partir do campo de discursividade existente em torno da criação e manutenção desta política nos governos de Fernando Henrique Cardoso (1995-2002). Para tal, a metodologia foi composta por uma abordagem baseada na teoria do discurso de Ernesto Laclau e Chantal Mouffe, tomando as práticas discursivas em torno do programa e evidenciando um contexto de disputas e de criação de novos lugares e não-lugares aos sujeitos envolvidos nas dinâmicas Estado-Sociedade.  Palavras-chave: Pronaf; Cidadania; Teoria do Discurso; Meio Rural.     Abstract  Program for the Strengthening of Family Farming (Pronaf) can be understood as the main response given by the State towards the institutionalization of citizenship in the countryside after the 1988 Constitution. This study aims to execute historical re-signification of the development of such program, starting from the discursive field around the creation and maintenance of the program in the mandates of Fernando Henrique Cardoso (1995-2002). For such, methodology was composed by an approach based on discourse theory of Ernesto Laclau and Chantal Mouffe, taking discursive practices around the program and evidencing a context of disputes and creation of new places and non-places to subjects involved in its dynamics.  Keywords: Pronaf; Citizenship; Discourse Theory; Countryside.     Resumem  Tomando el Programa Nacional de Fortalecimiento de la Agricultura Familiar (Pronaf) como la principal respuesta dada por el Estado a la institucionalización de la ciudadanía en el medio rural post-Constitución de 1988, este trabajo busca por medio de la división histórica demarcada de 1995-2002, la resignificación histórica del desarrollo de tal programa, a partir del campo de discursividad existente en torno a la creación y mantenimiento de esta política en los gobiernos de Fernando Henrique Cardoso (1995-2002). Para ello, la metodología se compuso de un enfoque basado en la teoría del discurso de Ernesto Laclau y Chantal Mouffe, tomando las prácticas discursivas en torno al programa y evidenciando un contexto de disputas y de creación de nuevos lugares y no lugares a los sujetos involucrados dinámicas Estado-Sociedad.  Palabras clave: Pronaf; Ciudadanía; Teoría del Discurso; Medio Rural.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2019</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="150452">
                <text>Simbiótica</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="150453">
                <text>Universidade Federal do Espírito Santo</text>
              </elementText>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>History of scholarship and learning. The humanities, Social sciences (General)</text>
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            <description>A related resource</description>
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                <text>&lt;a href="http://periodicos.ufes.br/simbiotica/article/view/28446" target="_blank" rel="noreferrer noopener"&gt;http://periodicos.ufes.br/simbiotica/article/view/28446&lt;/a&gt;</text>
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              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Agricultura sostenible</text>
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              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="88122">
                  <text>Dominio científico: Agricultura sostenible</text>
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              </elementTextContainer>
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          </elementContainer>
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      </elementSetContainer>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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      <elementSet elementSetId="1">
        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>A CIDADE E AS PAISAGENS CULTURAIS, RECURSOS NA LUTA CONTRA A POBREZA: ENTRE O CONTROLE SOCIETAL E O RECONHECIMENTO SOCIAL</text>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="216132">
                <text>Igor  Oliveira, Yara  Silveira</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="216133">
                <text>Com a revolução técnico-científica presente na temática da Globalização, novos espaços são criados/transformados, condicionados pelas manifestações e hábitos culturais. Desta forma, novos espaços são concebidos através das paisagens culturais e pela humanização do espaço vivido. Dentro dessa temática, a cidade apresenta-se como campo da materialização deste processo, uma vez que, nesse, apresenta-se como forma multicultural, abrigando diferentes crenças, línguas, valores, paisagem; tornando assim um campo perceptível às pesquisas. O trabalho segue-se dividido em seções, onde a princípio discute-se a questão cultural dentro do processo de globalização; em seguida, é realizada uma breve revisão do termo cultura a partir da visão de diferentes autores; posteriormente, é discutida a questão das paisagens culturais sob o olhar da geografia e por fim abordar-se-á a relação cultura e cidade, onde são apresentados dois exemplos de manifestações culturais na cidade: a relação de toponímia e Identidade, e a Agricultura Urbana.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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              <elementText elementTextId="216134">
                <text>2020</text>
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            </elementTextContainer>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="216135">
                <text>Cidade, Paisagem cultural, cultura, espaço e Identidade</text>
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            </elementTextContainer>
          </element>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="216136">
                <text>Revista Desenvolvimento Social</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="216137">
                <text>Programa de Pós-Graduação em Desenvolvimento Social - Universidade Estadual de Montes Claros</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="38">
            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
            <elementTextContainer>
              <elementText elementTextId="216138">
                <text>Social sciences (General)</text>
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            </elementTextContainer>
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            <name>Relation</name>
            <description>A related resource</description>
            <elementTextContainer>
              <elementText elementTextId="216139">
                <text>&lt;a href="https://www.periodicos.unimontes.br/index.php/rds/article/view/1615" target="_blank" rel="noreferrer noopener"&gt;https://www.periodicos.unimontes.br/index.php/rds/article/view/1615&lt;/a&gt;</text>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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              </elementTextContainer>
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            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="2">
                  <text>Dominio científico: Coronavirus</text>
                </elementText>
              </elementTextContainer>
            </element>
          </elementContainer>
        </elementSet>
      </elementSetContainer>
    </collection>
    <itemType itemTypeId="1">
      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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    <elementSetContainer>
      <elementSet elementSetId="1">
        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
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              <elementText elementTextId="8383">
                <text>A classification model for lncRNA and mRNA based on k-mers and a convolutional neural network</text>
              </elementText>
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          </element>
          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="8384">
                <text>Jianghui Wen, Yeshu Liu, Yu Shi, Haoran Huang, Bing Deng, Xinping Xiao</text>
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            </elementTextContainer>
          </element>
          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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              <elementText elementTextId="8385">
                <text>Abstract Background Long-chain non-coding RNA (lncRNA) is closely related to many biological activities. Since its sequence structure is similar to that of messenger RNA (mRNA), it is difficult to distinguish between the two based only on sequence biometrics. Therefore, it is particularly important to construct a model that can effectively identify lncRNA and mRNA. Results First, the difference in the k-mer frequency distribution between lncRNA and mRNA sequences is considered in this paper, and they are transformed into the k-mer frequency matrix. Moreover, k-mers with more species are screened by relative entropy. The classification model of the lncRNA and mRNA sequences is then proposed by inputting the k-mer frequency matrix and training the convolutional neural network. Finally, the optimal k-mer combination of the classification model is determined and compared with other machine learning methods in humans, mice and chickens. The results indicate that the proposed model has the highest classification accuracy. Furthermore, the recognition ability of this model is verified to a single sequence. Conclusion We established a classification model for lncRNA and mRNA based on k-mers and the convolutional neural network. The classification accuracy of the model with 1-mers, 2-mers and 3-mers was the highest, with an accuracy of 0.9872 in humans, 0.8797 in mice and 0.9963 in chickens, which is better than those of the random forest, logistic regression, decision tree and support vector machine.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="8386">
                <text>2019</text>
              </elementText>
            </elementTextContainer>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="8387">
                <text>lncRNA, mRNA, Kmers, relative entropy, convolutional neural network</text>
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            </elementTextContainer>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="8388">
                <text>DOI: 10.1186/s12859-019-3039-3</text>
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            </elementTextContainer>
          </element>
          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="8389">
                <text>BMC Bioinformatics</text>
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            </elementTextContainer>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="8390">
                <text>BMC</text>
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            </elementTextContainer>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="8391">
                <text>Biology (General), Computer applications to medicine. Medical informatics</text>
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            </elementTextContainer>
          </element>
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            <name>Language</name>
            <description>A language of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="8392">
                <text>EN</text>
              </elementText>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
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                <elementText elementTextId="2">
                  <text>Dominio científico: Coronavirus</text>
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              </elementTextContainer>
            </element>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>A clinical case of successful application of a new treatment method for severe COVID-19</text>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="83359">
                <text>Alexander S. Samoylov, Yuri D. Udalov, Nikolai M. Kruglyakov, Dmitry А. Terekhov, German I. Bagzhanov, Sergey S. Ochkin</text>
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                <text>Medicine</text>
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                <text>A Clinical Vignette on Community Transition after Inpatient Rehabilitation for a Veteran with New Spinal Cord Injury-Related Disability During the COVID-19 Pandemic.</text>
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                <text>Sameer Siddiqui, Donna Huang, Hilary Touchett, Felicia Skelton</text>
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                <text>American journal of physical medicine &amp; rehabilitation</text>
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