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                <text>Clozapine is strongly associated with the risk of pneumonia and inflammation</text>
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                <text>Jose de Leon, Chuan-Yue Wang, Hélène Verdoux, Can-Jun Ruan</text>
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                <text>Clinicians need to remember that (1) systemic inflammations can increase clozapine level; (2) clozapine, by itself, can cause inflammation, particularly during titration that is too rapid for that patient; (3) clozapine may increase the risk of infection; and (4) more specifically, clozapine may be particularly strongly associated with the risk of pneumonia. Pneumonia appears to be associated with high mortality in clozapine patients around the world. Clinicians who are alert to the risk of pneumonia in clozapine patients may significantly decrease mortality in clozapine patients. There is no data on COVID-19 infections in clozapine patients, but based on what we know about clozapine pharmacology, we can hypothesise that clozapine, possibly by impairing immunological mechanisms, may increase the risk of pneumonia in infected patients. More importantly, once fever and/or pneumonia develops, the clozapine dose should be cut in half to decrease the risk of clozapine intoxication. If there is any doubt that in spite of halving the dose there are still signs of clozapine intoxication, completely stopping clozapine may be indicated. Once the signs of inflammation and fever have disappeared, the clozapine dose can be increased to the prior dosage level.</text>
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                <text>DOI: 10.1136/gpsych-2019-100183</text>
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                <text>General Psychiatry</text>
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                <text>Jila  Yavarian, Farshid Rezaei, Azadeh  Shadab, Mahmood Soroush, Mohammad Mehdi Gooya, Talat Mokhtari-Azad</text>
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                <text>During January 2013–August 2014, a total of 1,800 patients in Iran who had respiratory illness were tested for Middle East respiratory syndrome coronavirus. A cluster of 5 cases occurred in Kerman Province during May–July 2014, but virus transmission routes for some infections were unclear.</text>
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                <text>DOI: 10.3201/eid2102.141405</text>
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                <text>Tze-wai Wong, Chin-Kei Lee, Wilson Tam, Joseph Tak-fai Lau, Tak-sun Yu, Siu-Fai Lui, Paul K. S. Chan, Yuguo Li, Joseph S. Bresee, Joseph J. Y. Sung, Umesh D Parashar</text>
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                <text>2004</text>
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                <text>severe acute respiratory syndrome, Transmission, superspreader, Hong Kong, research</text>
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                <text>DOI: 10.3201/eid1002.030452</text>
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                <text>Clusters of Coronavirus Disease in Communities, Japan, January–April 2020</text>
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                <text>Yuki Furuse, Eiichiro Sando, Naho Tsuchiya, Reiko Miyahara, Ikkoh Yasuda, Yura K. Ko, Mayuko Saito, Konosuke Morimoto, Takeaki Imamura, Yugo Shobugawa, Shohei Nagata, Kazuaki Jindai, Tadatsugu Imamura, Tomimasa Sunagawa, Motoi Suzuki, Hiroshi Nishiura, Hitoshi Oshitani</text>
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                <text>We analyzed 3,184 cases of coronavirus disease in Japan and identified 61 case-clusters in healthcare and other care facilities, restaurants and bars, workplaces, and music events. We also identified 22 probable primary case-patients for the clusters; most were 20–39 years of age and presymptomatic or asymptomatic at virus transmission.</text>
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                <text>Co-Circulation of Canine Coronavirus I and IIa/b with High Prevalence and Genetic Diversity in Heilongjiang Province, Northeast China.</text>
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                <text>Xinyu Wang, Chun-qiu Li, Donghua Guo, Shan Wei, Yufei Geng, Enyu Wang, Zhihui Wang, Xi Wen Zhao, Ming-Jun Su, Qiujin Liu, Siyao Zhang, Li Feng, Dongbo Sun</text>
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                <text>To trace the evolution of canine coronavirus (CCoV), 201 stool samples from diarrheic dogs in northeast China were subjected to reverse transcription-polymerase chain reactions (RT-PCRs) targeting the partial M and S genes of CCoV, followed by an epidemiological analysis. M gene RT-PCRs showed that 28.36% (57/201) of the samples were positive for CCoV; of the 57 positive samples, CCoV-I and CCoV-II accounted for 15.79% (9/57) and 84.21% (48/57), respectively. A sequence comparison of the partial M gene revealed nucleotide homologies of 88.4%-100% among the 57 CCoV strains, and 88.7%-96.2% identity between the 57 CCoV strains and the Chinese reference strain HF3. The CCoV-I and CCoV-II strains exhibited genetic diversity when compared with reference strains from China and other countries. The 57 CCoV strains exhibited high co-infection rates with canine kobuvirus (CaKV) (33.33%) and canine parvovirus-2 (CPV-2) (31.58%). The CCoV prevalence in diarrheic dogs differed significantly with immunization status, regions, seasons, and ages. Moreover, 28 S genes were amplified from the 57 CCoV-positive samples, including 26 CCoV-IIa strains, one CCoV-IIb strain, and one CCoV-I strain. A sequence comparison of the partial S gene revealed 86.3%-100% nucleotide identity among the 26 CCoV-IIa strains, and 89.6%-92.2% identity between the 26 CCoV-IIa strains and the Chinese reference strain V1. The 26 CCoV-IIa strains showed genetic diversity when compared with reference strains from China and other countries. Our data provide evidence that CCoV-I, CCoV-IIa, and CCoV-IIb strains co-circulate in the diarrhoetic dogs in northeast China, high co-infection rates with CaKV and CPV-2 were observed, and the CCoV-II strains exhibited high prevalence and genetic diversity.</text>
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                <text>DOI: 10.1371/journal.pone.0146975</text>
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                <text>PLoS ONE</text>
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                <text>Co-circulation of Four Human Coronaviruses (HCoVs) in Queensland Children with Acute Respiratory Tract Illnesses in 2004</text>
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                <text>Theo P Sloots, Ristan M Greer, Michael D. Nissen, Peter K. McErlean, Nicholas T. O’Neil, Katherine E. Arden, David J. Speicher, Ian M Mackay</text>
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                <text>Acute respiratory illnesses (ARIs) with unconfirmed infectious aetiologies peak at different times of the year. Molecular diagnostic assays reduce the number of unconfirmed ARIs compared to serology- or culture-based techniques. Screening of 888 inpatient and outpatient respiratory specimens spanning late autumn through to early spring, 2004, identified the presence of a human coronavirus (HCoV) on 74 occasions (8.3% of all specimens and 26.3% of all respiratory virus detections). Prevalence peaked in August (late winter in the southern hemisphere) when they were detected in 21.9% of specimens tested. HCoV-HKU1 and HCoV-OC43 comprised 82.4% of all HCoVs detected. Positive specimens were used to develop novel reverse transcriptase real-time PCRs (RT-rtPCRs) for HCoV detection. An objective clinical severity score was assigned to each positive HCoV patient. Severity scores were similar to those from a random selection of young children who were positive for respiratory syncytial virus at a different time but from the same specimen population. During the cooler months of 2004, sensitive and specific RT-rtPCRs identified the concurrent circulation of all four HCoVs, a quarter of which co-occurred with another virus and most of which were from children under the age of two years.</text>
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                <text>respiratory virus, coronavirus, HCoV-HKU1, HCoV-NL63, HCoV-229E, HCoV-OC43, real-time PCR, clinical impact</text>
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                <text>DOI: 10.3390/v4040637</text>
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                <text>Viruses</text>
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                <text>MDPI AG</text>
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                <text>Microbiology</text>
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                <text>Co-Circulation of the Rare CPV-2c with Unique Gln370Arg Substitution, New CPV-2b with Unique Thr440Ala Substitution, and New CPV-2a with High Prevalence and Variation in Heilongjiang Province, Northeast China.</text>
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                <text>Yufei Geng, Donghua Guo, Chun-qiu Li, Enyu Wang, Shan Wei, Zhihui Wang, Shuang Yao, Xi Wen Zhao, Ming-Jun Su, Xinyu Wang, Jianfa Wang, Rui Wu, Li Feng, Dongbo Sun</text>
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                <text>To trace evolution of canine parvovirus-2 (CPV-2), a total of 201 stool samples were collected from dogs with diarrhea in Heilongjiang province of northeast China from May 2014 to April 2015. The presence of CPV-2 in the samples was determined by PCR amplification of the VP2 gene (568 bp) of CPV-2. The results revealed that 95 samples (47.26%) were positive for CPV-2, and they showed 98.8%-100% nucleotide identity and 97.6%-100% amino acid identity. Of 95 CPV-2-positive samples, types new2a (Ser297Ala), new2b (Ser297Ala), and 2c accounted for 64.21%, 21.05%, and 14.74%, respectively. The positive rate of CPV-2 and the distribution of the new2a, new2b and 2c types exhibited differences among regions, seasons, and ages. Immunized dogs accounted for 48.42% of 95 CPV-2-positive samples. Coinfections with canine coronavirus, canine kobuvirus, and canine bocavirus were identified. Phylogenetic analysis revealed that the identified new2a, new2b, and CPV-2c strains in our study exhibited a close relationship with most of the CPV-2 strains from China; type new2a strains exhibited high variability, forming three subgroups; type new2b and CPV-2c strains formed one group with reference strains from China. Of 95 CPV-2 strains, Tyr324Ile and Thr440Ala substitutions accounted for 100% and 64.21%, respectively; all type new2b strains exhibited the Thr440Ala substitution, while the unique Gln370Arg substitution was found in all type 2c strains. Recombination analysis using entire VP2 gene indicated possible recombination events between the identified CPV-2 strains and reference strains from China. Our data revealed the co-circulation of new CPV-2a, new CPV-2b, and rare CPV-2c, as well as potential recombination events among Chinese CPV-2 strains.</text>
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                <text>DOI: 10.1371/journal.pone.0137288</text>
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                <text>PLoS ONE</text>
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                <text>Co-detection of SARS-CoV-2 with Secondary Respiratory Pathogen Infections.</text>
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                <text>Clifford L Freeman, Nathaniel M Miller, Lisa Bastarache, Josh Peterson, Wesley H Self, Tyler W Barrett, Michael J Ward</text>
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                <text>10.1007/s11606-020-06471-0</text>
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                <text>Wuhui Song, Xiaofang Jia, Xiaonan Zhang, Yun Ling, Zhigang Yi</text>
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            <name>Date</name>
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                <text>10.1016/j.jinf.2020.10.006</text>
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                <text>The Journal of infection</text>
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