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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Correlation of Automated Chemiluminescent Method with Enzyme-Linked Immunosorbent Assay (ELISA) Antibody Titers in Convalescent COVID-19 Plasma Samples: Development of Rapid, Cost-Effective Semi-Quantitative Diagnostic Methods</text>
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                <text>Mendoza R, Silver M, Zuretti AR, Christian M, Das B, Norin AJ, Borgen P, Libien J, Bluth MH</text>
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                <text>Rachelle Mendoza,1 Michael Silver,2,3 Alejandro R Zuretti,1,4 Manan Christian,4 Ballabh Das,5 Allen J Norin,5 Patrick Borgen,6 Jenny Libien,1,4 Martin H Bluth4 1Department of Pathology, SUNY Downstate Health Sciences University, Brooklyn, NY, USA; 2School of Public Health, SUNY Downstate Health Sciences University, Brooklyn, NY, USA; 3Department of Medicine, Maimonides Medical Center, Brooklyn, NY, USA; 4Department of Pathology, Maimonides Medical Center, Brooklyn, NY, USA; 5Department of Medicine, SUNY Downstate Health Sciences University, Brooklyn, NY, USA; 6Department of Surgery, Maimonides Medical Center, Brooklyn, NY, USACorrespondence: Martin H BluthBlood Transfusion and Donor Services, Clinical Laboratories, Translational Research (Pathology), Maimonides Medical Center, 4802 10 th Avenue, Brooklyn, NY, 11219, USAEmail MBluth@maimonidesmed.orgBackground: We investigated the utility of an automated chemiluminescent SARS-CoV-2 IgG antibody assay platform in quantifying the amount of binding antibodies present in donated convalescent plasma.Methods: A total of 179 convalescent plasma units were analyzed for the presence of SARS-CoV-2 IgG antibodies using the Beckman-Coulter chemiluminescent immunoassay (CLIA) platform. The equipment-derived numerical values (S/Co ratio) were recorded. Aliquots from the same units were subjected to enzyme-linked immunosorbent assay (ELISA) that detects IgG antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 S1 protein. The relationship between ELISA titers and CLIA S/Co values was analyzed using linear regression and receiver operating characteristics (ROC) curve.Results: Twenty-one samples (11.7%) had S/Co values of less than 1.0 and were deemed negative for antibodies and convalescent plasma had S/Co values between &amp;gt; 1.0 and 5.0 (70/179, 39.1%). Fifteen units (8.4%) had negative ELISA titer. The majority of the units (95/179. 53.1%) had titers &amp;ge; 1:1024. The sensitivities of ELISA to CLIA were comparable (90.5% vs 88.3%, respectively; p=0.18). There was positive linear correlation between CLIA S/Co values and ELISA IgG titer (Rho = 0.75; Spearman&amp;rsquo;s rank = 0.82, p-value = &amp;lt; 0.0001). The agreement between the two methods was fair, with a &amp;kappa; index of 0.2741. Using the ROC analysis, we identified a CLIA S/Co cutoff value of 8.2, which gives a sensitivity of 90% and a specificity of 82% in predicting a titer dilution of &amp;ge; 1:1024.Conclusion: The utility of automated antibody detection systems can be extended from simply a screening method to a semi-quantitative and quantitative functional antibody analysis. CLIA S/Co values can be used to reliably estimate the ELISA antibody titer. Incorporation of chemiluminescent-based methods can provide rapid, cost-effective means of identifying anti-SARS-CoV-2 antibody titers in donated plasma for use in the treatment of COVID-19 infection.Keywords: COVID-19, ELISA, chemiluminescence, antibody, titers</text>
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            <name>Date</name>
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                <text>2021</text>
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                <text>ELISA, covid-19, antibody, Chemiluminescence, titers</text>
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                <text>Biotemas</text>
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                <text>Universidade Federal de Santa Catarina</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Diseases of the blood and blood-forming organs</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Correlation of COVID-19 Pandemic with Healthcare System Response and Prevention Measures in Saudi Arabia</text>
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                <text>Heba  M. Adly, Imad  A. AlJahdali, Mohammed  A. Garout, Abdullah  A. Khafagy, Abdulla  A. Saati, Saleh  A. K. Saleh</text>
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                <text>Background: The Saudi government has taken the decision to prevent the entrance of about 2.5 million international pilgrims seeking to perform hajj in order to protect the world from a catastrophic widespread of disease. Moreover, health systems in Saudi Arabia are offering free testing for residents whether Saudi and non-Saudi. Objective: This study aimed to evaluate the spread of COVID-19 associated with preventive measures taken in Saudi Arabia and to develop a detailed COVID-19 prevention strategy as a framework for the Saudi Arabia community. Methodology: Population size and age distributions among the country of Saudi Arabia were taken from the 2020 World Population Prospects. Contact patterns were measured using the Saudi Arabia Ministry of Health Statistical Annual Report. Conclusions: Our study demonstrates that performing screening tests as early as possible to facilitate the rapid detection of infected cases, fast treatment, and instant isolation for suspected cases is the most definitive rejoinder for public health. Moreover, our study revealed the significance of performing preventive measures in reducing infection and death rates around Saudi Arabia by 27%, while in other countries, it reduced the death rate ranging from 10–73%. This study provides an achievable strategy for prevention and early detection of COVID-19 spread.</text>
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                <text>2020</text>
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                <text>Saudi Arabia, Prevention, covid-19, Pandemic, health care, organizational measures</text>
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                <text>10.3390/ijerph17186666</text>
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                <text>Epidemiology and Health</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>Medicine</text>
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                <text>Correspondence: Ebola Virus Disease in ASEAN Countries</text>
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                <text>Viroj Wiwanitkit, Beuy Joob</text>
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                <text>2016</text>
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                <text>zoonosis, Middle East respiratory syndrome, new emerging infection</text>
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                <text>DOI: 10.7860/JCDR/2016/17673.7546</text>
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                <text>Journal of Clinical and Diagnostic Research</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Correspondence: the donation of human milk during the COVID-19 pandemic</text>
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                <text>Pasqua Anna   Quitadamo</text>
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                <text>Dear Editor,   In previous epidemics, it has been shown that the use of breast milk represents one of the first crucial measures to be taken in emergencies.   On the occasion of the World Day for the Donation of Human Milk, which was celebrated on May 22, the Italian Society of Neonatology (SIN) reaffirmed the importance of Human Milk Banks (HMBs), reassuring mothers about the safety of donation at HMBs, also in this pandemic period.   In the Neonatal Intensive Care Unit (NICU) of San Giovanni Rotondo, Italy, we found that the restrictive measures did not represent a reason to limit breastfeeding nor donation. We have been close to nursing and donating mothers, providing constant support from the HMB and NICU staff, with practical advice and psychological closeness.   In particular, in the February-May period, the crucial one of the pandemic, 15 donors donated 91.25 liters. In comparison with the average of previous years of donation data to the HMB, the balance is even in favor of the current year. In fact, in 2019, in the same period, 12 mothers were recruited and 58.7 liters were collected and, in 2018, the donor women were 10 but they donated 119.5 liters.   As manager of the HMB, I found it useful to report the experience of our HMB and our NICU on this specific aspect, and I hope that other HMBs can do the same. I believe that these testimonies can represent an element of hope, encouragement and reflection. Solidarity did not end with the lockdown.</text>
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                <text>2020</text>
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                <text>covid-19, Italy, human milk, donation, milk bank, pre-terms</text>
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                <text>10.7363/100131</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>Medicine, Pediatrics</text>
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