85 10 20655 https://www.socictopen.socict.org/files/original/233386a77b846c41bed011dbd1fa855f.pdf 25d387b805c714cd981588c76c07179f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The small foreigner: new laws will promote the introduction of non-native zooplankton in Brazilian aquatic environments Creator An entity primarily responsible for making the resource Paula Nunes Coelho, Raoul Henry Description An account of the resource Abstract Non-native species of zooplankton have been introduced in Brazilian continental aquatic environments for a variety of reasons (e.g., non-native fish stocking, aquaculture), but many pathways of zooplankton introductions remain unknown. Recently, the governor of the State of Amazonas passed Law No. 79/2016, which allows for aquaculture operations using non-native fish in that state. This change in policy will likely result in the species introduction or propagule pressure of non-native zooplankton such as: Mesocyclops ogunnus Onabamiro, 1957; Kellicottia bostoniensis (Rousselet, 1908); Daphnia lumholtzi Sars, 1885; Lernaea cyprinacea (Linnaeus, 1758); Lamproglena monodi Capart, 1944. Of further concern is federal law No. 5989/09, which proposes the use of non-native fish in Brazilian aquaculture nationwide. We believe that both laws will intensify the introduction of non-native zooplankton. Once established in aquatic environments, non-native species of zooplankton could result in negative impacts (e.g., a loss of ecosystem services, food web alterations). Therefore, Brazilian authorities should incentivize the use of local native species rather than pass laws that threaten the environment. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource invasões biológicas, pressão de propágulo, Neotropical, Água doce, Política ambiental Identifier An unambiguous reference to the resource within a given context DOI: 10.1590/s2179-975x0717 Source A related resource from which the described resource is derived Acta Limnologica Brasiliensia Publisher An entity responsible for making the resource available Associação Brasileira de Limnologia Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Ecology Language A language of the resource EN https://www.socictopen.socict.org/files/original/2cdc073606302000a8cf11cc012bac34.pdf bd8446d235a126d6d12ab7c4a6fbea7f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Discovery and Characterization of Novel Bat Coronavirus Lineages from Kazakhstan Creator An entity primarily responsible for making the resource Ian H. Mendenhall, Aslan A. Kerimbayev, Vitaliy M. Strochkov, Kulyaisan T. Sultankulova, Syrym K. Kopeyev, Yvonne C.F. Su, Gavin JD Smith, Mukhit B. Orynbayev Description An account of the resource Coronaviruses are positive-stranded RNA viruses that infect a variety of hosts, resulting in a range of symptoms from gastrointestinal illness to respiratory distress. Bats are reservoirs for a high diversity of coronaviruses, and focused surveillance detected several strains genetically similar to MERS-coronavirus, SARS-coronavirus, and the human coronaviruses 229E and NL63. The bat fauna of central Asia, which link China to eastern Europe, are relatively less studied than other regions of the world. Kazakhstan is the world&#8217;s ninth largest country; however, little is understood about the prevalence and diversity of bat-borne viruses. In this study, bat guano was collected from bat caves in three different sites of southern Kazakhstan that tested positive for coronaviruses. Our phylogenetic reconstruction indicates these are novel bat coronaviruses that belong to the genus Alphacoronavirus. In addition, two distinct lineages of Kazakhstan bat coronaviruses were detected. Both lineages are closely related to bat coronaviruses from China, France, Spain, and South Africa, suggesting that co-circulation of coronaviruses is common in multiple bat species with overlapping geographical distributions. Our study highlights the need for collaborative efforts in understudied countries to increase integrated surveillance capabilities toward better monitoring and detection of infectious diseases. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource Kazakhstan, <i>Myotis blythii</i>, <i>Hypsugo savii</i>, coronavirus, virus discovery, phylogeny Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v11040356 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN https://www.socictopen.socict.org/files/original/3acca427ffb7b5fcdca3de01be2346ca.pdf 759d3c2ee02766216f6588b70a962836 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Canine Respiratory Coronavirus, Bovine Coronavirus, and Human Coronavirus OC43: Receptors and Attachment Factors Creator An entity primarily responsible for making the resource Artur Szczepanski, Katarzyna Owczarek, Monika Bzowska, Katarzyna Gula, Inga Drebot, Marek Ochman, Beata Maksym, Zenon Rajfur, Judy A Mitchell, Krzysztof Pyrc Description An account of the resource Despite high similarity of canine respiratory coronavirus (CRCoV), bovine coronavirus, (BCoV) and human coronavirus OC43 (HCoV-OC43), these viruses differ in species specificity. For years it was believed that they share receptor specificity, utilizing sialic acids for cell surface attachment, internalization, and entry. Interestingly, careful literature analysis shows that viruses indeed bind to the cell surface via sialic acids, but there is no solid data that these moieties mediate virus entry. In our study, using a number of techniques, we showed that all three viruses are indeed able to bind to sialic acids to a different extent, but these molecules render the cells permissive only for the clinical strain of HCoV-OC43, while for others they serve only as attachment receptors. CRCoV and BCoV appear to employ human leukocyte antigen class I (HLA-1) as the entry receptor. Furthermore, we identified heparan sulfate as an alternative attachment factor, but this may be related to the cell culture adaptation, as in ex vivo conditions, it does not seem to play a significant role. Summarizing, we delineated early events during CRCoV, BCoV, and HCoV-OC43 entry and systematically studied the attachment and entry receptor utilized by these viruses. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource canine respiratory coronavirus, Bovine coronavirus, human coronavirus OC43, attachment receptor, entry receptor, sialic acids, coronavirus, entry, HLA Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v11040328 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN https://www.socictopen.socict.org/files/original/6091fdafa4aa084ed5090f711945a15a.pdf 247033f1458fd224a64ca2ce78ce1ab4 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A Bibliometric Analysis of PubMed Literature on Middle East Respiratory Syndrome Creator An entity primarily responsible for making the resource Zhengting Wang, Yongdi Chen, Gaofeng Cai, Zhenggang Jiang, Kui Liu, Bin Chen, Jianmin Jiang, Hua Gu Description An account of the resource Middle East Respiratory Syndrome (MERS), a pandemic threat to human beings, has aroused huge concern worldwide, but no bibliometric studies have been conducted on MERS research. The aim of this study was to map research productivity on the disease based on the articles indexed in PubMed. The articles related to MERS dated from 2012 to 2015 were retrieved from PubMed. The articles were classified into three categories according to their focus. Publication outputs were assessed and frequently used terms were mapped using the VOS viewer software. A total of 443 articles were included for analysis. They were published in 162 journals, with Journal of Virology being the most productive (44 articles; 9.9%) and by six types of organizations, with universities being the most productive (276 articles; 62.4%).The largest proportion of the articles focused on basic medical sciences and clinical studies (47.2%) and those on prevention and control ranked third (26.2%), with those on other focuses coming in between (26.6%). The articles on prevention and control had the highest mean rank for impact factor (IF) (226.34), followed by those on basic medical sciences and clinical studies (180.23) and those on other focuses (168.03). The mean rank differences were statistically significant (p = 0.000). Besides, “conronavirus”, “case”, “transmission” and “detection” were found to be the most frequently used terms. The findings of this first bibliometric study on MERS suggest that the prevention and control of the disease has become a big concern and related research should be strengthened. Date A point or period of time associated with an event in the lifecycle of the resource 2016 Subject The topic of the resource Middle East respiratory syndrome, MERS, Literature review, Bibliometrics Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/ijerph13060583 Source A related resource from which the described resource is derived International Journal of Environmental Research and Public Health Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/21aa620ab9e707ebfdf9e4b68f98ff6b.pdf 68e7a4bad7d85424d9af6a9b934ed650 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection Creator An entity primarily responsible for making the resource Xingdong Zhou, Yingying Cong, Tineke Veenendaal, Judith Klumperman, Dongfang Shi, Muriel Mari, Fulvio Reggiori Description An account of the resource The porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) belonging to the α-CoV genus and it causes high mortality in infected sucking piglets, resulting in substantial losses in the farming industry. CoV trigger a drastic reorganization of host cell membranes to promote their replication and egression, but a detailed description of the intracellular remodeling induced by PEDV is still missing. In this study, we examined qualitatively and quantitatively, using electron microscopy, the intracellular membrane reorganization induced by PEDV over the course of an infection. With our ultrastructural approach, we reveal that, as most of CoV, PEDV initially forms double-membrane vesicles (DMVs) and convoluted membranes (CMs), which probably serve as replication/transcription platforms. Interestingly, we also found that viral particles start to form almost simultaneously in both the endoplasmic reticulum and the large virion-containing vacuoles (LVCVs), which are compartments originating from the Golgi, confirming that α-CoV assemble indistinguishably in two different organelles of the secretory pathway. Moreover, PEDV virons appear to have an immature and a mature form, similar to another α-CoV the transmissible gastroenteritis coronavirus (TGEV). Altogether, our study underlies the similarities and differences between the lifecycle of α-CoV and that of viruses belonging to other CoV subfamilies. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource PEDV, alphacoronavirus, life cycle, electron microscopy, membrane rearrangement Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v9090251 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN https://www.socictopen.socict.org/files/original/d9dbc03966421e4fc48c2ac2346fbeb0.pdf 5021b182b811c7fb8ec28681e682b275 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Epidemiological status of the Middle East respiratory syndrome coronavirus in 2019: an update from January 1 to March 31, 2019 Creator An entity primarily responsible for making the resource Ahmadzadeh J, Mobaraki K Description An account of the resource Jamal Ahmadzadeh, Kazhal MobarakiSocial Determinants of Health Research Center, Urmia University of Medical Sciences, Urmia, IranCorrespondence: Kazhal MobarakiEpidemiologist in Social Determinants of Health Research Center, Urmia University of Medical Sciences, Resalat Street, Urmia, IranTel +98 918 173 2869Fax +98 443 224 0642Email Mobaraki.k@umsu.ac.irPurpose: This study represents the current epidemiological status of Middle East respiratory syndrome coronavirus (MERS-CoV) worldwide in the first three months of 2019.Patients and methods: Full details of the MERS-CoV cases available and published in the disease outbreak news on the WHO website were retrieved. Related details of laboratory-confirmed MERS-CoV were extracted and analyzed by standard statistical methods.Results: A total of 107 cases of MERS-CoV, including 18 deaths (overall case fatality rate (CFR), 16.8%; male-specific CFR was 17.5% [14/80] and female-specific CFR was 14.8% [4/27]) were reported to WHO from the National International Health Regulation Focal Points of Saudi Arabia and Oman. The overall mean age was 50&plusmn;17 years and 80 patients (74.8%) were male. The average time from the onset of the symptoms to the first hospitalization was 3&plusmn;3.3 days; from the first hospitalization to laboratory confirmation was 3.6&plusmn;6.5 days; from the onset of symptom to death was 17.5&plusmn;11.7 days; and the mean length of hospitalization for&nbsp;patients with MERS-CoV was 3.5&plusmn;3.9 days. Males in comparison to females had a 1.5-fold increased chance (adjusted OR =1.5 [95% CI: 1.3&ndash;1.8]) of death related to MERS-CoV infection; 1.05 [95% CI: 1.1&ndash;3.3], 1.05 [95% CI: 1.2&ndash;2.8] and 1.06 [95% CI: 1.2&ndash;2.0] for those who had exposure to camels, camel milk consumption, and close contact with MERS-CoV cases, respectively. Health care workers had 2.4 fold [95% CI: 1.2&ndash;3.1] greater odds of death compared to other people.Conclusion: The knowledge obtained from this study can contribute to the development of a prevention program and early system warning against MERS-CoV infection.Keywords: Middle East respiratory syndrome coronavirus, emerging infectious disease, disease outbreaks Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource Middle East respiratory syndrome coronavirus, emerging infectious disease, Disease Outbreaks Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived International Journal of General Medicine Publisher An entity responsible for making the resource available Dove Medical Press Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine (General) Language A language of the resource EN https://www.socictopen.socict.org/files/original/bdb1682ed58589f8496331bf9377b4e2.pdf 3c254d7ce3027f203c00c82a33f4da08 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Abnormal Iron Homeostasis and Neurodegeneration Creator An entity primarily responsible for making the resource Barry B Muhoberac, Ruben eVidal Description An account of the resource Abnormal iron metabolism is observed in many neurodegenerative diseases, however only two have shown dysregulation of brain iron homeostasis as the primary cause of neurodegeneration. Herein, we review one of these - hereditary ferritinopathy (HF) or neuroferritinopathy, which is an autosomal dominant, adult onset degenerative disease caused by mutations in the ferritin light chain (FTL) gene. HF has a clinical phenotype characterized by a progressive movement disorder, behavioral disturbances, and cognitive impairment. The main pathologic findings are cystic cavitation of the basal ganglia, the presence of ferritin inclusion bodies (IBs), and substantial iron deposition. Mutant FTL subunits have altered sequence and length but assemble into soluble 24-mers that are ultrastructurally indistinguishable from those of the wild type. Crystallography shows substantial localized disruption of the normally tiny 4-fold pores between the ferritin subunits because of unraveling of the C-termini into multiple polypeptide conformations. This structural alteration causes attenuated net iron incorporation leading to cellular iron mishandling, ferritin aggregation, and oxidative damage at physiological concentrations of iron and ascorbate. A transgenic murine model parallels several features of HF, including a progressive neurological phenotype, ferritin IB formation, and misregulation of iron metabolism. These studies provide a working hypothesis for the pathogenesis of HF by implicating (1) a loss of normal ferritin function that triggers iron accumulation and overproduction of ferritin polypeptides, and (2) a gain of a toxic function through radical production, ferritin aggregation, and oxidative stress. Importantly, the finding that ferritin aggregation can be reversed by iron chelators and oxidative damage can be inhibited by radical trapping may be used for clinical investigation. This work provides new insights into the role of abnormal iron metabolism in neurodegeneration. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource Inclusion Bodies, Iron, oxidative stress, neurodegeneration, ferritin, neuroferritinopathy Identifier An unambiguous reference to the resource within a given context DOI: 10.3389/fnagi.2013.00032 Source A related resource from which the described resource is derived Frontiers in Aging Neuroscience Publisher An entity responsible for making the resource available Frontiers Media S.A. Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Neurosciences. Biological psychiatry. Neuropsychiatry Language A language of the resource EN https://www.socictopen.socict.org/files/original/9a1a0cba08bfbec306a0b9d7b4e3420a.pdf 42cd320ba106b33a36a4726e8ad418b8 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Life history, biomass and production of Coronatella rectangula (Branchiopoda, Anomopoda, Chydoridae) from Minas Gerais Creator An entity primarily responsible for making the resource Thais Viti, Célio Wisniewski, Tereza Cristina Orlando, Maria José dos Santos Wisniewski Description An account of the resource Species of Chydoridae provide the main diversity of the Cladocera. These organisms have been the subject of many studies; some dealing with their role in energy flow in aquatic ecosystems, since they inhabit the littoral region of water bodies which undergo the first impacts from anthropic activities. The aim of this study is to increase knowledge about the life cycle of Coronatella rectangula (Sars, 1861), a species found in several water bodies in the state of Minas Gerais, Brazil. The life cycle was determined by the culture of parthenogenetic females under controlled conditions in the laboratory. Experimental cultures were maintained in growth chambers at a constant temperature of 23.6(±0.5)ºC, through a 12 h light/12 h dark photoperiod. The organisms were fed on a suspension of Pseudokirchneriella subcapitata (Chlorophyceae) (10(5) cells.mL-1), and 0.02 mL of a mixed suspension of yeast and fish ration added per organism in equal proportions (1:1). Fifty parthenogenetic females with eggs were isolated and maintained until they produced neonates. Thirty of these neonates that had less than 24 hours were put in polypropylene bottles of 50 mL and kept in a germination chamber. These organisms were observed daily to obtain the parameters of the life cycle. Biomass and secondary production were also calculated. The embryonic development time of the specimens of C. rectangula was 1.68(±0.13) days and the time to reach primipara, was 2.48(±0.45) days. The mean fecundity of C. rectangula was two eggs/female/brood and the total number of eggs produced by the female during its life cycle was 27.8 eggs. During the whole life cycle, specimens of C. rectangula had a maximum of 14 seedlings, with two instars in the juvenile stage. Total biomass for C. rectangula was 36.66 µgDW.m-3(9.83 for the juvenile stage and 26.82 µgDW.m-3 for adults), and secondary production was 12.10 µgDW.m-3.day-1(8.34 µgDW.m-3.day-1 for egg production and 3.76 µgDW.m-3.day-1 for the juvenile stage). Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource ciclo de vida, Cladocera, região litoral, organismos fitófilos Identifier An unambiguous reference to the resource within a given context DOI: 10.1590/S0073-47212013000200005 Source A related resource from which the described resource is derived Iheringia: Série Zoologia Publisher An entity responsible for making the resource available Fundação Zoobotânica do Rio Grande do Sul Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Zoology Language A language of the resource EN, ES, PT https://www.socictopen.socict.org/files/original/e0c34816ba2407aa1aad28455c075415.pdf 094e86be26e483e3ea212de7ec9dd67c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Extracellular and intraneuronal HMW-AbetaOs represent a molecular basis of memory loss in Alzheimer's disease model mouse Creator An entity primarily responsible for making the resource Yamamoto Naoki, Urisu Tsuneo, Abe Koji, Sun Hui, Nabeshima Toshitaka, Mouri Akihiko, Yokoseki Tatsuki, Shibata Masao, Kawarabayashi Takeshi, Okamoto Yasuhide, Takamura Ayumi, Shoji Mikio, Yanagisawa Katsuhiko, Michikawa Makoto, Matsubara Etsuro Description An account of the resource Abstract Background Several lines of evidence indicate that memory loss represents a synaptic failure caused by soluble amyloid β (Aβ) oligomers. However, the pathological relevance of Aβ oligomers (AβOs) as the trigger of synaptic or neuronal degeneration, and the possible mechanism underlying the neurotoxic action of endogenous AβOs remain to be determined. Results To specifically target toxic AβOs in vivo, monoclonal antibodies (1A9 and 2C3) specific to them were generated using a novel design method. 1A9 and 2C3 specifically recognize soluble AβOs larger than 35-mers and pentamers on Blue native polyacrylamide gel electrophoresis, respectively. Biophysical and structural analysis by atomic force microscopy (AFM) revealed that neurotoxic 1A9 and 2C3 oligomeric conformers displayed non-fibrilar, relatively spherical structure. Of note, such AβOs were taken up by neuroblastoma (SH-SY5Y) cell, resulted in neuronal death. In humans, immunohistochemical analysis employing 1A9 or 2C3 revealed that 1A9 and 2C3 stain intraneuronal granules accumulated in the perikaryon of pyramidal neurons and some diffuse plaques. Fluoro Jade-B binding assay also revealed 1A9- or 2C3-stained neurons, indicating their impending degeneration. In a long-term low-dose prophylactic trial using active 1A9 or 2C3 antibody, we found that passive immunization protected a mouse model of Alzheimer's disease (AD) from memory deficits, synaptic degeneration, promotion of intraneuronal AβOs, and neuronal degeneration. Because the primary antitoxic action of 1A9 and 2C3 occurs outside neurons, our results suggest that extracellular AβOs initiate the AD toxic process and intraneuronal AβOs may worsen neuronal degeneration and memory loss. Conclusion Now, we have evidence that HMW-AβOs are among the earliest manifestation of the AD toxic process in mice and humans. We are certain that our studies move us closer to our goal of finding a therapeutic target and/or confirming the relevance of our therapeutic strategy. Date A point or period of time associated with an event in the lifecycle of the resource 2011 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1750-1326-6-20 Source A related resource from which the described resource is derived Molecular Neurodegeneration Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Neurology. Diseases of the nervous system, Geriatrics Language A language of the resource EN https://www.socictopen.socict.org/files/original/9a56a1ba01b5170afda80df8b306c440.pdf 89675062adb12ea805e21d7459e0d5ee Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Protective Role of Toll-like Receptor 3-Induced Type I Interferon in Murine Coronavirus Infection of Macrophages Creator An entity primarily responsible for making the resource Sonia Navas-Martin, Nneka Ikpeze, Julio Martin-Garcia, Liudmila Mazaleuskaya, Rogier Veltrop Description An account of the resource Toll-like Receptors (TLRs) sense viral infections and induce production of type I interferons (IFNs), other cytokines, and chemokines. Viral recognition by TLRs and other pattern recognition receptors (PRRs) has been proven to be cell-type specific. Triggering of TLRs with selected ligands can be beneficial against some viral infections. Macrophages are antigen-presenting cells that express TLRs and have a key role in the innate and adaptive immunity against viruses. Coronaviruses (CoVs) are single-stranded, positive-sense RNA viruses that cause acute and chronic infections and can productively infect macrophages. Investigation of the interplay between CoVs and PRRs is in its infancy. We assessed the effect of triggering TLR2, TLR3, TLR4, and TLR7 with selected ligands on the susceptibility of the J774A.1 macrophage cell line to infection with murine coronavirus (mouse hepatitis virus, [MHV]). Stimulation of TLR2, TLR4, or TLR7 did not affect MHV production. In contrast, pre-stimulation of TLR3 with polyinosinic-polycytidylic acid (poly I:C) hindered MHV infection through induction of IFN-β in macrophages. We demonstrate that activation of TLR3 with the synthetic ligand poly I:C mediates antiviral immunity that diminishes (MHV-A59) or suppresses (MHV-JHM, MHV-3) virus production in macrophages. Date A point or period of time associated with an event in the lifecycle of the resource 2012 Subject The topic of the resource murine coronavirus, macrophage, TLR, polyI:C, type I IFN,  antiviral state Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v4050901 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN