91 10 20655 https://www.socictopen.socict.org/files/original/e27509f5a4d48bd0187e8d5e889c61fc.pdf ae41c1fb5b23191805e370c7bdb3a9a4 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource An Outbreak of SARS in a Diabetes Room of a General Hospital without Infected Medical Staff Creator An entity primarily responsible for making the resource LIU Chun-ling, CHENG Jun, Gao He, Zhang Bo, Yuan Qun, Tong Ao, Liang Yi-an, Tang Hong, Guan Xiao-hong Description An account of the resource Objective To investigate the epidemiologic features of an outbreak of SARS that occurred in a single diabetes room of a general hospital in Beijing in late March 2003. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource SARS, Outbreak, nosocomial, disease transmission, droplet Identifier An unambiguous reference to the resource within a given context DOI: 10.1515/ii-2017-0060 Source A related resource from which the described resource is derived Infection International Publisher An entity responsible for making the resource available Sciendo Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/04aeb7d2d3a584268dd98fa0d688fdff.pdf 18913f9f26fb47285351c1f2ece90ddf Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Analyzing the MERS disease control strategy through an optimal control problem Creator An entity primarily responsible for making the resource Aldila Dipo, Padma Herningtyas, Khotimah Khusnul, Desjwiandra Bevina, Tasman Hengki Description An account of the resource A deterministic mathematical model of the Middle East respiratory syndrome (MERS) disease is introduced. Medical masks, supportive care treatment and a government campaign about the importance of medical masks will be involved in the model as time dependent variables. The problem is formulated as an optimal control one to minimize the number of infected people and keep the intervention costs as low as possible. Assuming that all control variables are constant, we find a disease free equilibrium point and an endemic equilibrium point explicitly. The existence and local stability criteria of these equilibria depend on the basic reproduction number. A sensitivity analysis of the basic reproduction number with respect to control parameters tells us that the intervention on medical mask use and the campaign about the importance of medical masks are much more effective for reducing the basic reproduction number than supportive care intervention. Numerical experiments for optimal control problems are presented for three different scenarios, i.e., a scenario of different initial conditions for the human population, a scenario of different initial basic reproduction numbers and a scenario of different budget limitations. Under budget limitations, it is much better to implement the medical mask intervention in the field, rather than give supportive care to control the spread of the MERS disease in the endemic prevention scenario. On the other hand, the medical mask intervention should be implemented partially together with supportive care to obtain the lowest number of infected people, with the lowest cost in the endemic reduction scenario. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource MERS, medical mask, Supportive care, campaign, Basic reproduction number, Optimal control Identifier An unambiguous reference to the resource within a given context DOI: 10.2478/amcs-2018-0013 Source A related resource from which the described resource is derived International Journal of Applied Mathematics and Computer Science Publisher An entity responsible for making the resource available Sciendo Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Mathematics, Electronic computers. Computer science Language A language of the resource EN https://www.socictopen.socict.org/files/original/0add15c39065b0be34fd2e13de399b27.pdf 4e026863fdc6cffa4971abd44f945313 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Aspects environnementaux du Livre vert de la Commission sur les mers et océans et de la communication sur la gestion intégrée des zones côtières Creator An entity primarily responsible for making the resource Phillippe Billet Date A point or period of time associated with an event in the lifecycle of the resource 2009 Subject The topic of the resource coastal zone, environment, ENVIRONNEMENT, Governance, integrated coastal management, Integrated management Identifier An unambiguous reference to the resource within a given context DOI: 10.4000/vertigo.8348 Source A related resource from which the described resource is derived VertigO Publisher An entity responsible for making the resource available Éditions en environnement VertigO Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Environmental sciences Language A language of the resource FR https://www.socictopen.socict.org/files/original/43ac6c66a0d279cfee8e5315ddbf6903.pdf d0e51087672177f081fd2c8781a6089a Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Clinical management of respiratory syndrome in patients hospitalized for suspected Middle East respiratory syndrome coronavirus infection in the Paris area from 2013 to 2016 Creator An entity primarily responsible for making the resource A. Bleibtreu, S. Jaureguiberry, N. Houhou, D. Boutolleau, H. Guillot, D. Vallois, J. C. Lucet, J. Robert, B. Mourvillier, J. Delemazure, M. Jaspard, F. X. Lescure, C. Rioux, E. Caumes, Y. Yazdanapanah Description An account of the resource Abstract Background Patients with suspected Middle East respiratory syndrome coronavirus (MERS-CoV) infection should be hospitalized in isolation wards to avoid transmission. This suspicion can also lead to medical confusion and inappropriate management of acute respiratory syndrome due to causes other than MERS-CoV. Methods We studied the characteristics and outcome of patients hospitalized for suspected MERS-CoV infection in the isolation wards of two referral infectious disease departments in the Paris area between January 2013 and December 2016. Results Of 93 adult patients (49 male (52.6%), median age 63.4 years) hospitalized, 82 out of 93 adult patients had returned from Saudi Arabia, and 74 of them were pilgrims (Hajj). Chest X-ray findings were abnormal in 72 (77%) patients. The 93 patients were negative for MERS-CoV RT-PCR, and 70 (75.2%) patients had documented infection, 47 (50.5%) viral, 22 (23.6%) bacterial and one Plasmodium falciparum malaria. Microbiological analysis identified Rhinovirus (27.9%), Influenza virus (26.8%), Legionella pneumophila (7.5%), Streptococcus pneumoniae (7.5%), and non-MERS-coronavirus (6.4%). Antibiotics were initiated in 81 (87%) cases, with two antibiotics in 63 patients (67.7%). The median duration of hospitalization and isolation was 3 days (1–33) and 24 h (8–92), respectively. Time of isolation decreased over time (P  Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource Middle East respiratory syndrome coronavirus (MERS-CoV), pilgrims, Saudi Arabia, Isolation ward, respiratory tract infection, Legionella Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12879-018-3223-5 Source A related resource from which the described resource is derived BMC Infectious Diseases Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/83f173c3355305ab6f08d4d7bce60ea2.pdf 069d3064299657bd79b26588d0caa8d8 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Comparative Subsequence Sets Analysis (CoSSA) is a robust approach to identify haplotype specific SNPs; mapping and pedigree analysis of a potato wart disease resistance gene Sen3 Creator An entity primarily responsible for making the resource Charlotte Prodhomme, Danny Esselink, Theo Borm, Richard G. F. Visser, Herman J. van Eck, Jack H. Vossen Description An account of the resource Abstract Background Standard strategies to identify genomic regions involved in a specific trait variation are often limited by time and resource consuming genotyping methods. Other limiting pre-requisites are the phenotyping of large segregating populations or of diversity panels and the availability and quality of a closely related reference genome. To overcome these limitations, we designed efficient Comparative Subsequence Sets Analysis (CoSSA) workflows to identify haplotype specific SNPs linked to a trait of interest from Whole Genome Sequencing data. Results As a model, we used the resistance to Synchytrium endobioticum pathotypes 2, 6 and 18 that co-segregated in a tetraploid full sib population. Genomic DNA from both parents, pedigree genotypes, unrelated potato varieties lacking the wart resistance traits and pools of resistant and susceptible siblings were sequenced. Set algebra and depth filtering of subsequences (k-mers) were used to delete unlinked and common SNPs and to enrich for SNPs from the haplotype(s) harboring the resistance gene(s). Using CoSSA, we identified a major and a minor effect locus. Upon comparison to the reference genome, it was inferred that the major resistance locus, referred to as Sen3, was located on the north arm of chromosome 11 between 1,259,552 and 1,519,485 bp. Furthermore, we could anchor the unanchored superscaffold DMB734 from the potato reference genome to a synthenous interval. CoSSA was also successful in identifying Sen3 in a reference genome independent way thanks to the de novo assembly of paired end reads matching haplotype specific k-mers. The de novo assembly provided more R haplotype specific polymorphisms than the reference genome corresponding region. CoSSA also offers possibilities for pedigree analysis. The origin of Sen3 was traced back until Ora. Finally, the diagnostic power of the haplotype specific markers was shown using a panel of 56 tetraploid varieties. Conclusions CoSSA is an efficient, robust and versatile set of workflows for the genetic analysis of a trait of interest using WGS data. Because the WGS data are used without intermediate reads mapping, CoSSA does not require the use of a reference genome. This approach allowed the identification of Sen3 and the design of haplotype specific, diagnostic markers. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource Bulked Segregant Analysis (BSA), whole genome sequencing (WGS), reference genome, Kmers, Diagnostic Markers, potato wart disease Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s13007-019-0445-5 Source A related resource from which the described resource is derived Plant Methods Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Plant culture Language A language of the resource EN https://www.socictopen.socict.org/files/original/bcf3037d4c86f51516bd1ff89efe535c.pdf 17acde306259da6254060c47dcec7ae7 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves Creator An entity primarily responsible for making the resource Danilo Tancler Stipp, Aline Fernandes Barry, Alice Fernandes Alfieri, Lívia Bodnar, Amauri Alcindo Alfieri Description An account of the resource Bovine coronavirus (BCoV) may cause acute diarrhea in newborn calves, leading to significant economic losses for cattle farmers. There are several diagnostic techniques used to detect BCoV in calf fecal samples, but virus isolation still has advantages for antigenic and genomic characterization. This study describes the isolation in HRT-18 cells and molecular characterization of Brazilian BCoV wild-type strains. Three fecal samples from diarrheic 30 day-old calves were inoculated in HRT-18 cell monolayers, which were then evaluated for HA titers and tested using semi-nested PCR followed by RFLP and sequencing. Two samples were successfully isolated and presented HA titers of 16 and 32 units per 25 mL. The results were confirmed using semi-nested PCR and RFLP. Molecular analyses identified a cell culture-adapted strain and a wild-type strain that were genetically similar (99%) to each other, but more distinct than BCoV strains circulating in other countries, even in the conserved N gene.O coronavírus bovino (BCoV) pode causar diarreia aguda em bezerros recém-nascidos, ocasionando consideráveis perdas econômicas para a pecuária bovina. Várias técnicas de diagnóstico podem ser empregadas na detecção do BCoV a partir de amostras fecais de bezerros. Porém, o isolamento do BCoV em cultivo celular apresenta a vantagem de possibilitar a caracterização antigênica e molecular da estirpe viral. O presente estudo descreve o isolamento em células HRT-18, e a caracterização molecular de estirpes brasileiras do BCoV. Três amostras de fezes diarreicas de bezerros com 30 dias de idade foram inoculadas em culturas de células HRT-18. Os isolados foram avaliados por hemaglutinação (HA) e por uma semi-nested PCR seguida de RFLP e sequenciamento. Duas amostras foram isoladas e a confirmação foi verificada na semi-nested PCR e também RFLP. Na HA os títulos foram de 16 e 32 unidades por 25 mL. Análises moleculares identificam a estirpe adaptada em cultura celular e uma estirpe selvagem, como estirpes de BCoV semelhantes (99%) entre si, mas distintas das circulantes em outros países, mesmo em um gene de uma proteína conservada (gene N). Date A point or period of time associated with an event in the lifecycle of the resource 2009 Subject The topic of the resource cattle, diarrhea, Bovine coronavirus, Cell culture, molecular analyses Identifier An unambiguous reference to the resource within a given context DOI: 10.1590/S1516-89132009000700007 Source A related resource from which the described resource is derived Brazilian Archives of Biology and Technology Publisher An entity responsible for making the resource available Instituto de Tecnologia do Paraná (Tecpar) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biotechnology Language A language of the resource EN https://www.socictopen.socict.org/files/original/36695d37b5c9c703bc51dc5d79f8e886.pdf 7a4e4069d542550f2d4d3da6512613b4 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel Targeting Sites Creator An entity primarily responsible for making the resource Li Liu, Shu-Hui Wang, Fan Yang, Yun Zhang, Ying Wang, Yingli Cao Description An account of the resource Small interfering RNA (siRNA) is a class of duplex RNA molecules of 21-25 nt nucleotides in length functioning post-transcriptionally to downregulate targeted gene expression. The membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is highly abundant during viral infections and is a critical element for viral assembly. Nucleotide substitution in the viral genome occurs frequently during SARS-CoV infection. In the current study, we analyzed the M gene sequences derived from 15 SARS-CoV isolates and uncovered six nucleotide substitutions among these isolates. Interestingly, these nucleotide substitutions are all located at the 5’ half of the M gene. Based on this information and previous reports, we created two novel siRNAs targeting two unexploited and well conserved regions in the M gene. The effects of these two siRNAs were tested by semi-quantitative RT-PCR and EGFP-M fusion gene expression. The results demonstrated that both siRNAs effectively and specifically blocked the targeted gene expression. Real time quantitative RT-PCR (qRT-PCR) revealed that siRNA targeting the 3’ half of the M gene (si-M2) induced more potent inhibition than that targeting the 5’ half (si-M1). Both si-M1 and si-M2 significantly downregulated M gene mediated upregulation of interferon b expression. Thus, our results indicate that SARS-CoV M gene specific siRNA might function in a sequence-dependent manner. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Subject The topic of the resource siRNA, SARS-CoV, membrane gene, RT-PCR, EGFP Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/molecules15107197 Source A related resource from which the described resource is derived Molecules Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Organic chemistry Language A language of the resource EN https://www.socictopen.socict.org/files/original/11ae97760d280ea10ab18755db75f890.pdf 847f3baee1a36d19658b555ee2ff745e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Risk of Introduction of Infectious Animal Diseases for Europe Based on the Health Situation of North Africa and the Arabian Peninsula Creator An entity primarily responsible for making the resource Elena Massó Sagüés, Eduardo Fernández-Carrión, José Manuel Sánchez-Vizcaíno Description An account of the resource The current growth of the human population, the intensification of animal production, climate change or globalization favors an increase in the transmission of infectious diseases. Risk analysis is the tool that allows the identification of the factors involved in the introduction and the spread of infectious diseases. The main objective of this work is to evaluate the risk of entry of animal infectious zoonotic and non-zoonotic diseases from North Africa and the Arabian Peninsula to countries of the European Union. A probabilistic formulation has been developed to obtain the probabilities of introduction of diseases associated with each possible route of entry in the European Union. The results show that, among the infectious diseases analyzed in this study, avian influenza and Newcastle disease are the ones with a higher risk of entry in the European Union and the wild bird's migration is the route with greater impact. It is confirmed a moderate probability of entry of some vector-borne diseases, bluetongue and epizootic haemorrhagic disease, through wind flow from Morocco, Algeria and Tunisia. Due to the absence of live dromedary movement to Europe, the more likely way of entry of the Middle East respiratory syndrome is through the infected people movement from Saudi Arabia, Kuwait, Qatar and Oman. This study includes different methodologies. A model of vectors dispersion in wind currents has been established to assess the risk of introduction of vector borne diseases. It is applicable both in animal health and public health. A periodical update would be useful to obtain a periodically updated risk analysis and to allow early detection of potential hazard with an increased risk over the previous years. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource Risk Analysis, infectious diseases, entry, Vector, Wind flow, birds migration Identifier An unambiguous reference to the resource within a given context DOI: 10.3389/fvets.2019.00293 Source A related resource from which the described resource is derived Frontiers in Veterinary Science Publisher An entity responsible for making the resource available Frontiers Media S.A. Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Veterinary medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/b3f3373fad768f6434f934a56044deaa.pdf 729cf3b542c6d0a802656b827b0a2cf5 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits Creator An entity primarily responsible for making the resource Ronaldo Magtoto, Korakrit Poonsuk, David Baum, Jian-Qiang Zhang, Qi Chen, Ju Ji, Pablo Piñeyro, Jeffrey Zimmerman, Luis G Giménez-Lirola Description An account of the resource Current measures to prevent TGEV from entering a naive herd include quarantine and testing for TGEV-seronegative animals. However, TGEV serology is complicated due to the cross-reactivity with PRCV, which circulates subclinically in most swine herds worldwide. Conventional serological tests cannot distinguish between TGEV and PRCV antibodies; however, blocking ELISAs using antigen containing a large deletion in the amino terminus of the PRCV S protein permit differentiation of PRCV and TGEV antibodies. Several commercial TGEV/PRCV blocking ELISAs are available, but performance comparisons have not been reported in recent research. This study demonstrates that the serologic cross-reactivity between TGEV and PRCV affects the accuracy of commercial blocking ELISAs. Individual test results must be interpreted with caution, particularly in the event of suspect results. Therefore, commercial TGEV/PRCV blocking ELISAs should only be applied on a herd basis.This study compared the performances of three commercial transmissible gastroenteritis virus/porcine respiratory coronavirus (TGEV/PRCV) blocking enzyme-linked immunosorbent assays (ELISAs) using serum samples (n = 528) collected over a 49-day observation period from pigs inoculated with TGEV strain Purdue (n = 12), TGEV strain Miller (n = 12), PRCV (n = 12), or with virus-free culture medium (n = 12). ELISA results were evaluated both with “suspect” results interpreted as positive and then as negative. All commercial kits showed excellent diagnostic specificity (99 to 100%) when testing samples from pigs inoculated with virus-free culture medium. However, analyses revealed differences between the kits in diagnostic sensitivity (percent TGEV- or PRCV-seropositive pigs), and all kits showed significant (P  Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource ELISA, Transmissible gastroenteritis virus, Antibody, cross-reactivity, porcine respiratory coronavirus, serum, swine Identifier An unambiguous reference to the resource within a given context DOI: 10.1128/mSphere.00017-19 Source A related resource from which the described resource is derived mSphere Publisher An entity responsible for making the resource available American Society for Microbiology Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN https://www.socictopen.socict.org/files/original/26869ee46c0ee4d636cde8ce6c6bc546.pdf 4a0a21c6908c315ad2e2175671f92b4c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Determination of Apparent Protein Molecular Weight in Solution by Diffusion Ordered NMR Spectroscopy Creator An entity primarily responsible for making the resource Lihong Wei, YUAN Zhi-liang, XIA Bin Description An account of the resource Diffusion ordered NMR spectroscopy (DOSY) can be used to determine the apparent molecular weight of proteins (Mprotein) by measuring self-diffusion coefficient (Dt-protein). However, Dt-protein is also affected by the property of buffer solution, making the measurements complicated. In this work, 3-(trimethylsilyl) propane sodium sulfonate (DSS) was used as the internal reference molecule and the diffusion coefficient ratio between the target protein and DSS (Dr) was used to characterize the protein in solution. This method reduced the effects of buffer system on Mprotein determination, yielding more accurate Mprotein measurement. The Dr values of reference proteins with different molecular weight were determined. A correlation curve between Dr and Mprotein was established (lgMprotein=-2.6488 lgDr-0.7863, R2=0.997). Finally, Dr of the C-terminal domain of SARS-CoV main protease (Mpro-C) was determined and to yield its molecular weight. Accuracy and practicability of the fitting curve were demonstrated. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource liquid-state NMR, apparent molecular weight, diffusion ordered NMR spectroscopy (DOSY), buffer solution, pulse gradient Identifier An unambiguous reference to the resource within a given context DOI: 10.11938/cjmr20182625 Source A related resource from which the described resource is derived Chinese Journal of Magnetic Resonance Publisher An entity responsible for making the resource available Science Press Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Electricity and magnetism Language A language of the resource ZH