955 10 20655 https://www.socictopen.socict.org/files/original/9db9e12a5d6712002889593e7fe888a7.pdf 5706f8db7c5d737f2f18a4701180f5a3 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus Creator An entity primarily responsible for making the resource Paul Kellam, Matthew Cotten, Andrew Rambaut, Oliver G. Pybus, Yi Guan, Eleni Nastouli, Tommy T. Lam, Deenan Pillay, Velislava Petrova, Simon J. Watson, Anne L. Palser, Paul Grant Description An account of the resource A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient’s sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource evolution, Respiratory tract infections, disease transmission, Molecular, Infectious, coronavirus infections Identifier An unambiguous reference to the resource within a given context DOI: 10.3201/eid1905.130057 Source A related resource from which the described resource is derived Emerging Infectious Diseases Publisher An entity responsible for making the resource available Centers for Disease Control and Prevention Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases, Medicine https://www.socictopen.socict.org/files/original/2e07828f6d5cff256899a32ae6825e6a.pdf 36d6f5a3e5e8bb566ac79800eb87289e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Full-genome sequences of the first two SARS-CoV-2 viruses from India Creator An entity primarily responsible for making the resource Pragya D Yadav, Megha Agrawal, Atanu Basu, Priya Abraham, Dimpal A. Nyayanit, Triparna D Majumdar, Sarah S Cherian, Varsha A Potdar, Anita Shete-Aich, Santosh M Jadhav, Manohar Lal Choudhary Description An account of the resource Background & objectives: Since December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has globally affected 195 countries. In India, suspected cases were screened for SARS-CoV-2 as per the advisory of the Ministry of Health and Family Welfare. The objective of this study was to characterize SARS-CoV-2 sequences from three identified positive cases as on February 29, 2020. Methods: Throat swab/nasal swab specimens for a total of 881 suspected cases were screened by E gene and confirmed by RdRp (1), RdRp (2) and N gene real-time reverse transcription-polymerase chain reactions and next-generation sequencing. Phylogenetic analysis, molecular characterization and prediction of B- and T-cell epitopes for Indian SARS-CoV-2 sequences were undertaken. Results: Three cases with a travel history from Wuhan, China, were confirmed positive for SARS-CoV-2. Almost complete (29,851 nucleotides) genomes of case 1, case 3 and a fragmented genome for case 2 were obtained. The sequences of Indian SARS-CoV-2 though not identical showed high (~99.98%) identity with Wuhan seafood market pneumonia virus (accession number: NC 045512). Phylogenetic analysis showed that the Indian sequences belonged to different clusters. Predicted linear B-cell epitopes were found to be concentrated in the S1 domain of spike protein, and a conformational epitope was identified in the receptor-binding domain. The predicted T-cell epitopes showed broad human leucocyte antigen allele coverage of A and B supertypes predominant in the Indian population. Interpretation & conclusions: The two SARS-CoV-2 sequences obtained from India represent two different introductions into the country. The genetic heterogeneity is as noted globally. The identified B- and T-cell epitopes may be considered suitable for future experiments towards the design of vaccines and diagnostics. Continuous monitoring and analysis of the sequences of new cases from India and the other affected countries would be vital to understand the genetic evolution and rates of substitution of the SARS-CoV-2. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource epitope - genomes - india - kerala - next-generation sequencing - phylogeny - real-time reverse transcription-polymerase chain reaction - severe acute respiratory syndrome coronavirus 2 Identifier An unambiguous reference to the resource within a given context DOI: 10.4103/ijmr.IJMR_663_20 Source A related resource from which the described resource is derived Indian Journal of Medical Research Publisher An entity responsible for making the resource available Wolters Kluwer Medknow Publications Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine https://www.socictopen.socict.org/files/original/5f8648b308e8d38cae3fd9621d5c393f.pdf 0edb53265d140d9c027cd3a19df40dd6 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs. Creator An entity primarily responsible for making the resource Thomas Bruun Rasmussen, Maria Beatrice Boniotti, Alice Papetti, Béatrice Grasland, Jean-Pierre Frossard, Akbar Dastjerdi, Marcel Hulst, Dennis Hanke, Anne Pohlmann, Sandra Blome, Wim H.M. van der Poel, Falko Steinbach, Yannick Blanchard, Antonio Lavazza, Anette Bøtner, Graham J Belsham Description An account of the resource Porcine epidemic diarrhoea virus, strain CV777, was initially characterized in 1978 as the causative agent of a disease first identified in the UK in 1971. This coronavirus has been widely distributed among laboratories and has been passaged both within pigs and in cell culture. To determine the variability between different stocks of the PEDV strain CV777, sequencing of the full-length genome (ca. 28kb) has been performed in 6 different laboratories, using different protocols. Not surprisingly, each of the different full genome sequences were distinct from each other and from the reference sequence (Accession number AF353511) but they are >99% identical. Unique and shared differences between sequences were identified. The coding region for the surface-exposed spike protein showed the highest proportion of variability including both point mutations and small deletions. The predicted expression of the ORF3 gene product was more dramatically affected in three different variants of this virus through either loss of the initiation codon or gain of a premature termination codon. The genome of one isolate had a substantially rearranged 5´-terminal sequence. This rearrangement was validated through the analysis of sub-genomic mRNAs from infected cells. It is clearly important to know the features of the specific sample of CV777 being used for experimental studies. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Identifier An unambiguous reference to the resource within a given context DOI: 10.1371/journal.pone.0193682 Source A related resource from which the described resource is derived PLoS ONE Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Science, Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/05990868061081d4f4b017825147795f.pdf 86733282ac7134c221ad6c852af563d3 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Full-length three-dimensional structure of the influenza A virus M1 protein and its organization into a matrix layer. Creator An entity primarily responsible for making the resource Lisa Selzer, Zhaoming Su, Grigore D Pintilie, Wah Chiu, Karla Kirkegaard Description An account of the resource Matrix proteins are encoded by many enveloped viruses, including influenza viruses, herpes viruses, and coronaviruses. Underneath the viral envelope of influenza virus, matrix protein 1 (M1) forms an oligomeric layer critical for particle stability and pH-dependent RNA genome release. However, high-resolution structures of full-length monomeric M1 and the matrix layer have not been available, impeding antiviral targeting and understanding of the pH-dependent transitions involved in cell entry. Here, purification and extensive mutagenesis revealed protein-protein interfaces required for the formation of multilayered helical M1 oligomers similar to those observed in virions exposed to the low pH of cell entry. However, single-layered helical oligomers with biochemical and ultrastructural similarity to those found in infectious virions before cell entry were observed upon mutation of a single amino acid. The highly ordered structure of the single-layered oligomers and their likeness to the matrix layer of intact virions prompted structural analysis by cryo-electron microscopy (cryo-EM). The resulting 3.4-Å-resolution structure revealed the molecular details of M1 folding and its organization within the single-shelled matrix. The solution of the full-length M1 structure, the identification of critical assembly interfaces, and the development of M1 assembly assays with purified proteins are crucial advances for antiviral targeting of influenza viruses. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Identifier An unambiguous reference to the resource within a given context 10.1371/journal.pbio.3000827 Source A related resource from which the described resource is derived PLoS Biology Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General) https://www.socictopen.socict.org/files/original/1facf207d909a89025bb7e8ba7870026.pdf fa8db27569e20c12e4c8635b28e84f5c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Fulminant hepatic failure in a patient testing re-positive for SARS-CoV-2: a case report Creator An entity primarily responsible for making the resource Hind S. Alsaif, Ali Hassan, Bader Aldossary, Mohamed Moussa, Dunya Alfaraj Description An account of the resource Abstract Background Infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may not elicit lifelong protective immunity and reinfection could occur. Liver function impairment is a common manifestation of coronavirus disease 2019 (COVID-19). However, acute hepatic failure in the setting of COVID-19 is very rare. Case presentation We report the case of a 47-year-old woman who presented with acute abdominal pain and vomiting. Abdominal examination revealed a soft and lax abdomen with mild tenderness in the right upper quadrant. The patient recovered from COVID-19 2 months previously with negative results on reverse transcription-polymerase chain reaction (RT-PCR). Laboratory investigations revealed markedly elevated transaminases with normal results on viral hepatitis serology panel and undetectable blood paracetamol level. Prior to admission, the patient underwent RT-PCR for SARS-CoV-2, which revealed a positive result. The patient experienced rapid deterioration in the neurological status with a remarkable increase in the liver enzyme levels. Despite aggressive resuscitation, the patient suffered irreversible cardiac arrest and died. Conclusion Fulminant hepatic failure is a rare manifestation in patients with re-positive RT-PCR tests for SARS-CoV-2. Clinicians should maintain a high index of suspicion for hepatic injury with active monitoring of liver enzymes. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource covid-19, Case reports, Reinfection, Liver failure, Re-positive test Identifier An unambiguous reference to the resource within a given context 10.1186/s12245-021-00349-6 Source A related resource from which the described resource is derived International Journal of Emergency Medicine Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medical emergencies. Critical care. Intensive care. First aid https://www.socictopen.socict.org/files/original/9c8c8cd6f60e7fe13af4929838051f9e.pdf b867ed1d2925a626d046a53ae6dd7410 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Fulminant micro and macroangiopathic sequalae in a patient with COVID-19. Creator An entity primarily responsible for making the resource Sanjay S Bhandari, Jian Yeo, Deevia Kotecha, Gerry P McCann Date A point or period of time associated with an event in the lifecycle of the resource 2020 Identifier An unambiguous reference to the resource within a given context 10.1093/ehjcr/ytaa372 Source A related resource from which the described resource is derived European heart journal. Case reports https://www.socictopen.socict.org/files/original/b3ea33cfa576f92c6405c34ff0f38df6.pdf 271fbf44e623aa4e14bb393c4be65e3f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Funciones de costos internos y externos en un modelo estratégico de transporte de carga Creator An entity primarily responsible for making the resource Luis Gabriel Márquez Díaz Description An account of the resource Se revisan las funciones de costos internos y externos,con fines de modelación estratégica del transportede carga. Se estudian el costo interno del tiempo, elcosto interno de operación y cinco componentes delcosto externo: costo externo de la congestión, costoexterno de los accidentes, costos de la polución delaire, costo del cambio climático y costo externo dela infraestructura; a partir de estos se deriva la funciónde costo marginal total. Se encuentra que es posibleestimar de forma empírica los parámetros de lasfunciones aplicables al transporte de cargainterregional en Colombia en los modos de transportecarretero, ferroviario y fluvial, y con ellos estudiarlos costos marginales sobre una red estratégica detransporte de carga. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Source A related resource from which the described resource is derived Revista Facultad de Ingeniería Publisher An entity responsible for making the resource available Universidad Pedagógica y Tecnológica de Colombia Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Engineering (General). Civil engineering (General) Relation A related resource <a href="http://revistas.uptc.edu.co/revistas/index.php/ingenieria/article/view/1368" target="_blank" rel="noreferrer noopener">http://revistas.uptc.edu.co/revistas/index.php/ingenieria/article/view/1368</a> https://www.socictopen.socict.org/files/original/4ae6fd532c290fea63f806ff88468908.pdf 1b62e062c0fe2ac2364575b28bda2620 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Funciones de los servicios ecosistémicos en los sistemas ganaderos en Cuba Creator An entity primarily responsible for making the resource Milagros de la Caridad Milera-Rodríguez Description An account of the resource Objetivo: Analizar las funciones de los servicios ecosistémicos en los sistemas ganaderos para la producción de alimentos de forma sostenible en Cuba.Materiales y Métodos: Se analizó la situación actual de los servicios ecosistémicos como componentes de los sistemas ganaderos, con prioridad en los de aprovisionamiento, como son el suelo, la biodiversidad de especies, los recursos zoogenéticos, el agua y la energía.Resultados:En cualquier sistema de producción de alimentos, el enfoque sistémico y la valoración acerca del cuidado de los servicios ecosistémicos es crucial, ya que constituyen la fuerza motriz para la protección del medio ambiente y el bienestar humano. Si bien todos los servicios ecosistémicos participan de manera importante, es esencial el cuidado del suelo, el agua y la biodiversidad porque ellos son la base de la pirámide que soporta todos los recursos ecosistémicos. Existen evidencias innovadoras en sistemas de producción agropecuarios sobre bases agroecológicas que avalan la necesidad de cambiar la manera de pensar, de habitar y transitar hacia una reconversión de los sistemas convencionales.Conclusiones:Los servicios ecosistémicos se enfocan desde una visión integradora, y es el manejo agroecológico el que permite la comprensión de sus beneficios. La agroecología se fundamenta en el cuidado de los recursos naturales y considera la multidimensionalidad de los sistemas y las interacciones, al incluir el factor humano y el conocimiento local, elementos que hacen viable la seguridad alimentaria. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource agroecología; integración; manejo sostenible Source A related resource from which the described resource is derived Pastos y Forrajes Publisher An entity responsible for making the resource available Estación Experimental de Pastos y Forrajes Indio Hatuey Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Agriculture (General), Animal culture Relation A related resource <a href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0864-03942021000100022&amp;lng=es&amp;nrm=iso&amp;tlng=es" target="_blank" rel="noreferrer noopener">http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0864-03942021000100022&amp;lng=es&amp;nrm=iso&amp;tlng=es</a> https://www.socictopen.socict.org/files/original/74e2a4ef3e23046424e4bc89ebe06f9d.pdf 76dd367748bd511522c1d6c025b8ab06 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Functional and Genetic Analysis of Coronavirus Replicase-Transcriptase Proteins. Description An account of the resource The coronavirus replicase-transcriptase complex is an assembly of viral and cellular proteins that mediate the synthesis of genome and subgenome-sized mRNAs in the virus-infected cell. Here, we report a genetic and functional analysis of 19 temperature-sensitive (ts) mutants of Murine hepatitis virus MHV-A59 that are unable to synthesize viral RNA when the infection is initiated and maintained at the non-permissive temperature. Both classical and biochemical complementation analysis leads us to predict that the majority of MHV-A59 ORF1a replicase gene products (non-structural proteins nsp1-nsp11) form a single complementation group (cistron1) while the replicase gene products encoded in ORF1b (non-structural proteins nsp12-nsp16) are able to function in trans and comprise at least three, and possibly five, further complementation groups (cistrons II-VI). Also, we have identified mutations in the non-structural proteins nsp 4, nsp5, nsp10, nsp12, nsp14, and nsp16 that are responsible for the ts phenotype of eight MHV-A59 mutants, which allows us to conclude that these proteins are essential for the assembly of a functional replicase-transcriptase complex. Finally, our analysis of viral RNA synthesis in ts mutant virus-infected cells allows us to discriminate three phenotypes with regard to the inability of specific mutants to synthesize viral RNA at the non-permissive temperature. Mutant LA ts6 appeared to be defective in continuing negative-strand synthesis, mutant Alb ts16 appeared to form negative strands but these were not utilized for positive-strand RNA synthesis, and mutant Alb ts22 was defective in the elongation of both positive- and negative-strand RNA. On the basis of these results, we propose a model that describes a pathway for viral RNA synthesis in MHV-A59-infected cells. Further biochemical analysis of these mutants should allow us to identify intermediates in this pathway and elucidate the precise function(s) of the viral replicase proteins involved. Date A point or period of time associated with an event in the lifecycle of the resource 2005 Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived PLoS Pathogens Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Immunologic diseases. Allergy Language A language of the resource EN https://www.socictopen.socict.org/files/original/ae4ff2801aa5937b6aadf175ec08a1e6.pdf 76dd367748bd511522c1d6c025b8ab06 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Functional and Genetic Analysis of Coronavirus Replicase-Transcriptase Proteins. Description An account of the resource The coronavirus replicase-transcriptase complex is an assembly of viral and cellular proteins that mediate the synthesis of genome and subgenome-sized mRNAs in the virus-infected cell. Here, we report a genetic and functional analysis of 19 temperature-sensitive (ts) mutants of Murine hepatitis virus MHV-A59 that are unable to synthesize viral RNA when the infection is initiated and maintained at the non-permissive temperature. Both classical and biochemical complementation analysis leads us to predict that the majority of MHV-A59 ORF1a replicase gene products (non-structural proteins nsp1-nsp11) form a single complementation group (cistron1) while the replicase gene products encoded in ORF1b (non-structural proteins nsp12-nsp16) are able to function in trans and comprise at least three, and possibly five, further complementation groups (cistrons II-VI). Also, we have identified mutations in the non-structural proteins nsp 4, nsp5, nsp10, nsp12, nsp14, and nsp16 that are responsible for the ts phenotype of eight MHV-A59 mutants, which allows us to conclude that these proteins are essential for the assembly of a functional replicase-transcriptase complex. Finally, our analysis of viral RNA synthesis in ts mutant virus-infected cells allows us to discriminate three phenotypes with regard to the inability of specific mutants to synthesize viral RNA at the non-permissive temperature. Mutant LA ts6 appeared to be defective in continuing negative-strand synthesis, mutant Alb ts16 appeared to form negative strands but these were not utilized for positive-strand RNA synthesis, and mutant Alb ts22 was defective in the elongation of both positive- and negative-strand RNA. On the basis of these results, we propose a model that describes a pathway for viral RNA synthesis in MHV-A59-infected cells. Further biochemical analysis of these mutants should allow us to identify intermediates in this pathway and elucidate the precise function(s) of the viral replicase proteins involved. Date A point or period of time associated with an event in the lifecycle of the resource 2005 Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived PLoS Pathogens Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Immunologic diseases. Allergy Language A language of the resource EN