122 10 20655 https://www.socictopen.socict.org/files/original/c4db01f327ff77da5cb4fed674cff08a.pdf 610859b49636066cc64aedd46cdcd25b Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The global burden of premature mortality due to the Middle East respiratory syndrome (MERS) using standard expected years of life lost, 2012 to 2019 Creator An entity primarily responsible for making the resource Maryam Salamatbakhsh, Kazhal Mobaraki, Sara Sadeghimohammadi, Jamal Ahmadzadeh Description An account of the resource Abstract Background It has been 8 years since the first case of Middle East respiratory syndrome coronavirus (MERS-CoV) was reported in Saudi Arabia and the disease is still being reported in 27 countries; however, there is no international study to estimate the overall burden related of this emerging infectious disease. The present study was conducted to assess the burden of premature mortality due to Middle East respiratory syndrome (MERS) worldwide. Methods In this retrospective analysis, we have utilized publicly available data from the WHO website related to 1789 MERS patients reported between September 23, 2012 and May 17, 2019. To calculate the standard expected years of life lost (SEYLL), life expectancy at birth was set according to the 2000 global burden of disease study on levels 25 and 26 of West model life tables from Coale-Demeny at 82.5 and 80 years for females and males, respectively. Results Overall, the total SEYLL in males and females was 10,702 and 3817.5 years, respectively. The MERS patients within the age range of 30–59 year-olds had the highest SEYLL (8305.5 years) in comparison to the patients within the age groups 0–29 (SEYLL = 3744.5 years) and ≥ 60 years (SEYLL = 2466.5 years). The total SEYLL in all age groups in 2012, 2013, 2014, 2015, 2016, 2017, 2018, and 2019 were 71.5, 2006.5, 3162, 4425.5, 1809.5, 878, 1257.5 and 909 years, respectively. The most SEYLL related to MERS-CoV infection was in the early four years of the onset of the pandemic (2012 to 2015) and in the last four years of the MERS-CoV pandemic (216 to 2019), a significant reduction was observed in the SEYLL related to MERS-CoV infection in the MERS patients. Conclusion We believe that the findings of this study will shed light about the burden of premature mortality due to MERS infection in the world and the results may provide necessary information for policy-makers to prevent, control, and make a quick response to the outbreak of MERS-CoV disease. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource Standard expected years of life, PREMATURE MORTALITY, burden of disease, worldwide Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12889-019-7899-2 Source A related resource from which the described resource is derived BMC Public Health Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Public aspects of medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/6bf1094fdb9c2b29bd48cd064d7d7363.pdf 21e50f2c7d0bb783d4a711050ef9fd45 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource ETIOPATHOGENETIC TREATMENT OF INFLUENZA AND ARVI IN CHILDREN: A NEW LOOK AT THE OLD PROBLEM Creator An entity primarily responsible for making the resource O. V. Shamsheva Description An account of the resource A new value was acquired by the drug Orvirem®, which is a combination of rimantadine and natrium alginate. In recent years, it has not been recommended for use due to the resistance to it strains of the influenza A(H1N1) virus pdm2009. Currently, this drug has acquired a new meaning, and this is due to the dominance of subtype A(H3N2). Two recent clinical studies have demonstrated that the drug Orvirem® has proven activity against viruses not only of influenza A but also B, coronavirus, and other common pathogens of respiratory viral infections (rhinoviruses, respiratory syncytial virus, adenovirus, etc.). High efficacy, safety and absence of adverse reactions make it possible to widely use Orvirem® in children from the first year of life, incl. with chronic diseases. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource antiviral drugs, Respiratory viruses, acute respiratory viral infections (arvi), influenza, rimantadine Identifier An unambiguous reference to the resource within a given context DOI: 10.22627/2072-8107-2017-16-3-49-54 Source A related resource from which the described resource is derived Detskie Infekcii (Moskva) Publisher An entity responsible for making the resource available LLC Diagnostics and Vaccines"" Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Pediatrics Language A language of the resource RU https://www.socictopen.socict.org/files/original/d77ebd507ddd43fc8c17d03a19a9ca83.pdf a1744a5a06326c02f5005b3dcf85277a Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource PANDEMIC INFLUENZA A (H1N1) PDM 09 IN THE SEASON 2015/2016: ANALYSIS OF FATAL OUTCOMES IN CHILDREN Creator An entity primarily responsible for making the resource V.N. Timchenko, O. I. Afanas'eva, E.A. Dondurey, O. V. Bulina, V. S. Afanas'eva, L. M. Pochinyaeva, E. F. Gorbunov Description An account of the resource The analysis of the characteristics of the current influenza A(H1N1) pdm 09 at 624 children in the 2015/2016 season. For severe illness (5%) characterized by short incubation period, lack of initial period, acute or acute onset with rapid development of multiorgan failure, prolonged febrile body temperature (up to 5—6 days or more) in the absence of complications, a prolonged severe intoxication (4—5 days or more), a significant frequency of diarrhea syndrome (10,2%), hemorrhagic syndrome (5.3%), exacerbation of comorbidity (20%), combined  with other viral infections  — herpes, rotavirus, enterovirus and others (33%).In the clinical analysis of blood — leukopenia (33.9%), leukocytosis (14.5%) with neutrophilic  shift (11.3%), neutrophilia (51.6%), monocytosis (45.2%), lymphocytosis (9.7%), thrombocytopenia, anemia, accelerated erythrocyte sedimentation rate moderately; in the biochemical analysis of blood, hypernatremia, increase in the concentration of lactate, hyperfermentemia (ALT, AST, ALP, LDH, CPK), hypoproteinemia, dysproteinemia, increased CRP, increased creatinine and urea, increase of procalcitonin (PCT). In the structure of complications of pneumonia was 6%, stenosing laryngotracheitis — 9.8%, obstructive bronchitis — 14.5%. Two unvaccinated against influenza in infants who become ill during the epidemic period for influenza and SARS from homes with a typical clinical picture, died from severe (toxic) form of pandemic influenza. Pandemic influenza occurred at the background of severe immunosuppression (atrophy of the thymus, depletion  of follicles of peripheral organs of immunogenesis — spleen, lymph nodes, tonsils). The main cause of death was the development of multiple organ failure with rapid progressive lesion of the vital organs. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource pandemic influenza a(h1n1) pdm 09, severe (toxic) degree, infants, MULTIPLE ORGAN FAILURE, immunosuppression, lethal outcomes Identifier An unambiguous reference to the resource within a given context DOI: 10.22627/2072-8107-2017-16-1-36-42 Source A related resource from which the described resource is derived Detskie Infekcii (Moskva) Publisher An entity responsible for making the resource available LLC Diagnostics and Vaccines"" Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Pediatrics Language A language of the resource RU https://www.socictopen.socict.org/files/original/55115ab70dcbc380be50478124380431.pdf 4f946dba98cba9b5acfc8c82835a9188 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource EFFECT OF BACTERIAL AGENTS OF PORCINE RESPIRATORY DISEASE COMPLEX ON PRODUCTIVE INDICES AND SLAUGHTER WEIGHT Creator An entity primarily responsible for making the resource Talita Brombilla, Renato Akio Ogata, Alessandra Figueiredo de Castro Nassar, Maristela Vasconcelos Cardoso, Vera Letticie de Azevedo Ruiz, Claudia Del Fava Description An account of the resource Porcine respiratory disease complex comprises the interaction of two or more infectious agents. The major bacterial agents involved were investigated in 115 finishing pigs at a farm in São Paulo State, Brazil: Actinobacillus pleuropneumoniae (serology, bacterial culture, and multiplex PCR), Mycoplasma hyopneumoniae (Mhyo) (nested PCR), Pasteurella multocida (multiplex PCR), Haemophilus parasuis (PCR multiplex), and Streptococcus sp. (bacterial culture). Macroscopic and microscopic lung lesions were evaluated, and zootechnical indices were recorded. Mhyo occurred in 113 animals (98.3%), seventeen of which were co-infected with Streptococcus sp. The finding of emphysematous lung was associated with significantly lower final and carcass weight at slaughter. Although vaccinated against Mhyo with an inactivated immunogen, almost 100% of the animals were infected. Mhyo infection with and without Streptococcus sp. co-infection was related to lung lesions of varying degrees and lower slaughter and carcass weight. Keywords: differential diagnosis, Mycoplasma hyopneumoniae, pleuropneumonia, Streptococcus sp., swine Date A point or period of time associated with an event in the lifecycle of the resource 2019 Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived Ciência Animal Brasileira Publisher An entity responsible for making the resource available Universidade Federal de Goiás Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Agriculture, Animal culture Language A language of the resource EN, PT https://www.socictopen.socict.org/files/original/39f609d293b1526f869792c6fae67d15.pdf cab0dc1b0a311fbe791573fc7b144089 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Risk of transmission via medical employees and importance of routine infection-prevention policy in a nosocomial outbreak of Middle East respiratory syndrome (MERS): a descriptive analysis from a tertiary care hospital in South Korea Creator An entity primarily responsible for making the resource Hyun Kyun Ki, Sang-Kuk Han, Jun Seong Son, Sang-O Park Description An account of the resource Abstract Background In 2015, South Korea experienced an outbreak of Middle East respiratory syndrome (MERS), and our hospital experienced a nosocomial MERS infection. We performed a comprehensive analysis to identify the MERS transmission route and the ability of our routine infection-prevention policy to control this outbreak. Methods This is a case–cohort study of retrospectively analysed data from medical charts, closed-circuit television, personal interviews and a national database. We analysed data of people at risk of MERS transmission including 228 in the emergency department (ED) and 218 in general wards (GW). Data of personnel location and movement, personal protection equipment and hand hygiene was recorded. Transmission risk was determined as the extent of exposure to the index patient: 1) high risk: staying within 2 m; 2) intermediate risk: staying in the same room at same time; and 3) low risk: only staying in the same department without contact. Results The index patient was an old patient admitted to our hospital. 11 transmissions from the index patient were identified; 4 were infected in our hospital. Personnel in the ED exhibited higher rates of compliance with routine infection-prevention methods as observed objectively: 93% wore a surgical mask and 95.6% washed their hands. Only 1.8% of personnel were observed to wear a surgical mask in the GW. ED had a higher percentage of high-risk individuals compared with the GW (14.5% vs. 2.8%), but the attack rate was higher in the GW (16.7%; l/6) than in the ED (3%; 1/33). There were no transmissions in the intermediate- and low-risk groups in the ED. Otherwise 2 patients were infected in the GW among the low-risk group. MERS were transmitted to them indirectly by staff who cared for the index patient. Conclusions Our study provide compelling evidence that routine infection-prevention policies can greatly reduce nosocomial transmission of MERS. Conventional isolation is established mainly from contact tracing of patients during a MERS outbreak. But it should be extended to all people treated by any medical employee who has contact with MERS patients. Trial registration NCT02605109, date of registration: 11th November 2015. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource Middle East respiratory syndrome coronavirus, nosocomial infection, infection control, isolation, Hand Hygiene Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12890-019-0940-5 Source A related resource from which the described resource is derived BMC Pulmonary Medicine Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Diseases of the respiratory system Language A language of the resource EN https://www.socictopen.socict.org/files/original/e0cc86c554be1eacb000538a6052ab98.pdf ce5c49c3bf967ce2eab56d81d2142e6a Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Camel production systems in Ethiopia: a review of literature with notes on MERS-CoV risk factors Creator An entity primarily responsible for making the resource Tadele Mirkena, Elias Walelign, Nega Tewolde, Getachew Gari, Getachew Abebe, Scott Newman Description An account of the resource Abstract Camels are the most adapted species to the harsh conditions of arid/semi-arid rangelands of Ethiopia where pastoralism is the dominant mode of life and mobility is an inherent strategy to efficiently utilize the spatially and temporally distributed pasture and water resources. Usually, large numbers of camels and other domestic animals from many different herds/flocks congregate at watering sites, and this may create a perfect condition for disease transmission and spread among animals. The same water sources are also shared by multitudes of wild animals. Camel herd sizes per household range from few heads (five to ten) to several hundreds. Female camels account for more than 75% of the herd. Male camels are usually sold early as pack animals or for slaughter. Female camels may remain fertile up to 25 years, during which time they produce eight to ten calves. Camels are herded during daytime on communal rangelands. During night, they are kept in traditional kraals around homesteads. Breeding time is short and seasonal and is affected by rainfall patterns and feed availability. Usually, only men milk camels. Milking frequency ranges from two to five times per day. Washing of hands, milking vessels, the udder and teats is not practised by many prior to milking the camels. Besides, the milking area is generally full of dust and dung and without shade. This affects the quality and safety of the produced milk. Pathogens and diseases of camelids are less well known; however, they are suspected as zoonotic sources for the human infection with the Middle East respiratory syndrome coronavirus. There is an increasing need to determine whether camels are clinically susceptible, act as potential reservoirs and maintenance or bridge hosts, to viral pathogens. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource ethiopia, dromedary camel, production system, pastoralism, MERS-CoV Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s13570-018-0135-3 Source A related resource from which the described resource is derived Pastoralism Publisher An entity responsible for making the resource available SpringerOpen Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Animal culture Language A language of the resource EN https://www.socictopen.socict.org/files/original/6f5b0d90e7e048710add177c162999ea.pdf 18bda791e2f0745b1c45a992f20291e7 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource MODERN APPROACHES TO THERAPY OF ACUTE RESPIRATORY VIRAL INFECTIONS OF THE UPPER RESPIRATORY TRACT Creator An entity primarily responsible for making the resource N.V. Orlova, I. I. Chukaeva Description An account of the resource Acute respiratory viral infection(ARVI) are the most common diseases  in the world. Acute respiratory viral infections (ARI), including influenza, include a group of infectious diseases of the respiratory tract with a viral etiology and are predominantly airborne transmission. SARS and flu are manifested in the diversity of respiratory symptoms. One of the rules of the medicine is the desired effect with a minimum number of indicated drugs. However, the patient is forced to administer a number of drugs aimed at the relief of coughing, running nose, headache, decrease in temperature, as well as antiviral, antihistamine and other medications. On the background of such polypharmacy use of combined drugs is reasonable. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource acute respiratory viral infections, Pharmacotherapy, combination medications, theraflu Identifier An unambiguous reference to the resource within a given context DOI: 10.21518/2079-701X-2017-5-58-64 Source A related resource from which the described resource is derived Медицинский совет Publisher An entity responsible for making the resource available Remedium Group LLC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource RU https://www.socictopen.socict.org/files/original/099ca1e5f8fbce315f55dd869afad7da.pdf d7f8668b912f984eef2527179404d9d8 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource ACUTE RHINITIS: NEW THERAPEUTIC POSSIBILITIES Creator An entity primarily responsible for making the resource A. A. Krivopalov, S.V. Ryazantsev, V. A. Shatalov, S. V. Shervashidze Description An account of the resource According to the World Health Organization pathology of the respiratory system ranks first (in some countries, second) in the structure of morbidity of the population worldwide. The complexity of the treatment, and growth of the unreasonable prescription of many drugs, leading to increased problems of polypragmasie, antibioticoresistance, allergization of the population, etc., encourages physicians to reconsider the boundaries of drug therapy and persistently look for new opportunities for effective and safe therapy. An open multicentre study was conduced to assess the effectiveness and tolerability of Coryzalia® in the treatment of acute infectious rhinitis, that showed that the complex homeopathic drug quickly relieves the main symptoms of acute infectious rhinitis on the background of SARS. By the 3rd day of therapy such symptoms as headache, rhinorrhea, nasal obstruction, sneezingare reduced twice. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource acute respiratory viral infection, acute rhinitis, complex homeopathic preparation, coryzalia® Identifier An unambiguous reference to the resource within a given context DOI: 10.21518/2079-701X-2017-8-18-23 Source A related resource from which the described resource is derived Медицинский совет Publisher An entity responsible for making the resource available Remedium Group LLC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource RU https://www.socictopen.socict.org/files/original/8e5b3995111b6187328e8c455478de9d.pdf e856f14825d8c4f2a79ac20c6681aa7d Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A putative diacidic motif in the SARS-CoV ORF6 protein influences its subcellular localization and suppression of expression of co-transfected expression constructs Creator An entity primarily responsible for making the resource Gunalan Vithiagaran, Mirazimi Ali, Tan Yee-Joo Description An account of the resource Abstract Background The ORF6 protein is one of the eight accessory proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV). Numerous properties of ORF6 have been documented and this study focuses on two of these, namely, its ability to suppress the expression of co-transfected expression constructs and its subcellular localization to vesicular structures. Results Using a transient transfection system, ORF6's ability to suppress the expression of co-transfected expression constructs was measured in a quantitative manner. While ORF6 does not have a global effect on protein synthesis, quantitative real-time PCR revealed that it down-regulated the mRNA level of the co-transfected myc-nsp8 gene. Furthermore, alanine substitution of a diacidic cluster motif (aa53-56) in the ORF6 gene caused a reduction in the suppression of expression of co-transfected myc-nsp8 gene. Our previous study revealed that ORF6 localized to vesicular structures in SARS-CoV infected Vero E6 cells. Here, ORF6 was observed to be localized to similar vesicular structures in Vero E6 cells which have been transiently transfected with a mammalian expression plasmid encoding for untagged ORF6. ORF6 showed partial colocalization with cellular proteins CD63 and Lamp1, suggesting that the vesicular structures may be a subpopulation of endosomal/lysosomal vesicles. The alanine substitution of the diacidic cluster motif also altered the subcellular localization of the ORF6 protein, indicating a potential relationship between the subcellular localization of the ORF6 protein and its ability to suppress the expression of co-transfected expression constructs. Conclusions By combining quantitative real-time PCR and transient transfection system, a simple and safe method is established to measure ORF6's ability to suppress the expression of co-transfected myc-nsp8. In addition, immunofluorescence analysis revealed that the subcellular localization of ORF6 when expressed on its own is similar to that observed in SARS-CoV infected cells. Through the use of these two assays, a putative diacidic motif in the ORF6 protein was found to influence its subcellular localization and ability to suppress the expression of co-transfected expression constructs. Date A point or period of time associated with an event in the lifecycle of the resource 2011 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1756-0500-4-446 Source A related resource from which the described resource is derived BMC Research Notes Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Science (General), Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/3cc1acc572bef78de33c709a376298b0.pdf c5514b0bc36ad6f5ac85abe2d7d18e48 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Time Course and Cellular Localization of SARS-CoV Nucleoprotein and RNA in Lungs from Fatal Cases of SARS. Description An account of the resource BACKGROUND: Cellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) in the lungs of patients with SARS is important in confirming the etiological association of the virus with disease as well as in understanding the pathogenesis of the disease. To our knowledge, there have been no comprehensive studies investigating viral infection at the cellular level in humans. METHODS AND FINDINGS: We collected the largest series of fatal cases of SARS with autopsy material to date by merging the pathological material from two regions involved in the 2003 worldwide SARS outbreak in Hong Kong, China, and Toronto, Canada. We developed a monoclonal antibody against the SARS-CoV nucleoprotein and used it together with in situ hybridization (ISH) to analyze the autopsy lung tissues of 32 patients with SARS from Hong Kong and Toronto. We compared the results of these assays with the pulmonary pathologies and the clinical course of illness for each patient. SARS-CoV nucleoprotein and RNA were detected by immunohistochemistry and ISH, respectively, primarily in alveolar pneumocytes and, less frequently, in macrophages. Such localization was detected in four of the seven patients who died within two weeks of illness onset, and in none of the 25 patients who died later than two weeks after symptom onset. CONCLUSIONS: The pulmonary alveolar epithelium is the chief target of SARS-CoV, with macrophages infected subsequently. Viral replication appears to be limited to the first two weeks after symptom onset, with little evidence of continued widespread replication after this period. If antiviral therapy is considered for future treatment, it should be focused on this two-week period of acute clinical disease. Date A point or period of time associated with an event in the lifecycle of the resource 2006 Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived PLoS Medicine Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource EN