121 10 20655 https://www.socictopen.socict.org/files/original/87f74166405ed6067593220df43038a6.pdf b610bc0bccb67c7da64bfc05d214a1c2 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A new record and redescription of Schizopera (Schizopera) knabeni (Copepoda: Harpacticoida: Miraciidae) from north-western Mexico Un registro nuevo y redescripción completa de Schizopera (Schizopera) knabeni (Copepoda: Harpacticoida: Miraciidae) del noroeste de México Creator An entity primarily responsible for making the resource Samuel Gómez, Febe Elizabeth Vargas-Arriaga Description An account of the resource Specimens of Schizopera Sars, 1905 were found during sampling in 2 brackish systems in central and southern Sinaloa (north-western Mexico). The specimens turned out to belong to Schizopera (Schizopera) knabeni Lang, 1965 which was originally described from Monterey Bay (the type locality) and is known from Cocodrie (Louisiana). Amendments to Lang's (1965) original description and Fiers and Rutledge's (1990) partial redescription are given.Durante una serie de muestreos se hallaron ejemplares del género Schizopera Sars, 1905 en 2 sistemas salobres en el centro y sur de Sinaloa (México). El material recolectado resultó ser Schizopera (Schizopera) knabeni Lang, 1965, una especie de copépodo harpacticoide originalmente descrita de material recolectado en la Bahía de Monterey (localidad tipo) y hallada también en Cocodrie (Louisiana, EUA). Se proporcionan enmiendas a la descripción original de Lang (1965) y a la redescripción parcial de Fiers y Rutledge (1990). Date A point or period of time associated with an event in the lifecycle of the resource 2008 Subject The topic of the resource Copepoda, Harpacticoida, Schizopera, Noroeste de México, taxonomia, northwestern Mexico, taxonomy Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived Revista Mexicana de Biodiversidad Publisher An entity responsible for making the resource available Universidad Nacional Autónoma de México Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General) Language A language of the resource EN, ES https://www.socictopen.socict.org/files/original/92f2f63544ebbdc1ca93465b69734690.pdf 49dcf817b14b9f0b25cbd1fbb05ca32e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Mapping and visualisation on of health data. The contribution on of the graphic sciences to medical research from New York yellow fever to China Coronavirus. Creator An entity primarily responsible for making the resource Enrico Cicalò, Michele Valentino Description An account of the resource This paper will discuss the role of data visualization in the field of medical science and the relationships between health research and graphic sciences. Graphic representation allows the survey and the visualization of intangible phenomena. For this reason, computer graphics and technologically innovative imaging are now gaining a central role in all the disciplines based on the analysis of phenomena that occur in the territory and from which decision making depends. Thus, knowledge, techniques and tools of the graphic sciences are increasingly being asked to contribute to interdisciplinary research. Health data visualization can be a useful tool to reveal new insights on the spatial patterns of disease spread, mainly in the study of risk factors for diseases considered “environmental” because a considerable part of their spread can be attributed to environmental factors so that their distribution patterns result strongly associated with the spatially heterogeneous environment to which they are referred. The simultaneous visualization of health data with environmental data obtained from different sources can further the understanding of environmental-health linkages and can generate new hypotheses to be tested in future research. Disease mapping and environmental risk assessment using digital geospatial data resources are now established analytical tools in both human and veterinary public health. Participatory GIS, Volunteered Geographic Information communities as OpenStreetMap, Virtual Globes, online live tracking dashboards and other computer-assisted applications made it possible to translate datasets from different sources and users into maps easily understandable from the public. The use of these tools in geospatial health has been firmly established as a useful tool for collating, exploring, visualizing and graphically analyzing health data. As a result, new approaches aimed to visualize, describe and explain the spatial patterns of diseases are being developed in different research fields, that will be analyzed in this paper.  Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource maps, Data visualisation, geospatial visualisation, graphic representation, Medical research Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived Disegnare con Publisher An entity responsible for making the resource available University of L'Aquila Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Architecture, Architectural drawing and design Language A language of the resource EN, ES, IT, PT https://www.socictopen.socict.org/files/original/1cef6cf8b9ccadc6f6fc267437d001a7.pdf 2100de55312d420d236bdf159b13841d Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Assessing the performance of different approaches for functional and taxonomic annotation of metagenomes Creator An entity primarily responsible for making the resource Javier Tamames, Marta Cobo-Simón, Fernando Puente-Sánchez Description An account of the resource Abstract Background Metagenomes can be analysed using different approaches and tools. One of the most important distinctions is the way to perform taxonomic and functional assignment, choosing between the use of assembly algorithms or the direct analysis of raw sequence reads instead by homology searching, k-mer analysys, or detection of marker genes. Many instances of each approach can be found in the literature, but to the best of our knowledge no evaluation of their different performances has been carried on, and we question if their results are comparable. Results We have analysed several real and mock metagenomes using different methodologies and tools, and compared the resulting taxonomic and functional profiles. Our results show that database completeness (the representation of diverse organisms and taxa in it) is the main factor determining the performance of the methods relying on direct read assignment either by homology, k-mer composition or similarity to marker genes, while methods relying on assembly and assignment of predicted genes are most influenced by metagenomic size, that in turn determines the completeness of the assembly (the percentage of read that were assembled). Conclusions Although differences exist, taxonomic profiles are rather similar between raw read assignment and assembly assignment methods, while they are more divergent for methods based on k-mers and marker genes. Regarding functional annotation, analysis of raw reads retrieves more functions, but it also makes a substantial number of over-predictions. Assembly methods are more advantageous as the size of the metagenome grows bigger. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource metagenomics, functional annotation, Taxonomic annotation, assembly Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12864-019-6289-6 Source A related resource from which the described resource is derived BMC Genomics Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Genetics, Biotechnology Language A language of the resource EN https://www.socictopen.socict.org/files/original/3b63ecc9d479d3df7cd647824ae750a5.pdf dbc61fb0fa27efc573545a10055aa0ca Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Analytical parameters and validation of homopolymer detection in a pyrosequencing-based next generation sequencing system Creator An entity primarily responsible for making the resource Gergely Ivády, László Madar, Erika Dzsudzsák, Katalin Koczok, János Kappelmayer, Veronika Krulišová, Milan Macek, Attila Horváth, István Balogh Description An account of the resource Abstract Background Current technologies in next-generation sequencing are offering high throughput reads at low costs, but still suffer from various sequencing errors. Although pyro- and ion semiconductor sequencing both have the advantage of delivering long and high quality reads, problems might occur when sequencing homopolymer-containing regions, since the repeating identical bases are going to incorporate during the same synthesis cycle, which leads to uncertainty in base calling. The aim of this study was to evaluate the analytical performance of a pyrosequencing-based next-generation sequencing system in detecting homopolymer sequences using homopolymer-preintegrated plasmid constructs and human DNA samples originating from patients with cystic fibrosis. Results In the plasmid system average correct genotyping was 95.8% in 4-mers, 87.4% in 5-mers and 72.1% in 6-mers. Despite the experienced low genotyping accuracy in 5- and 6-mers, it was possible to generate amplicons with more than a 90% adequate detection rate in every homopolymer tract. When homopolymers in the CFTR gene were sequenced average accuracy was 89.3%, but varied in a wide range (52.2 – 99.1%). In all but one case, an optimal amplicon-sequencing primer combination could be identified. In that single case (7A tract in exon 14 (c.2046_2052)), none of the tested primer sets produced the required analytical performance. Conclusions Our results show that pyrosequencing is the most reliable in case of 4-mers and as homopolymer length gradually increases, accuracy deteriorates. With careful primer selection, the NGS system was able to correctly genotype all but one of the homopolymers in the CFTR gene. In conclusion, we configured a plasmid test system that can be used to assess genotyping accuracy of NGS devices and developed an accurate NGS assay for the molecular diagnosis of CF using self-designed primers for amplification and sequencing. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource pyrosequencing, Homopolymer detection, Cystic fibrosis Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12864-018-4544-x Source A related resource from which the described resource is derived BMC Genomics Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Genetics, Biotechnology Language A language of the resource EN https://www.socictopen.socict.org/files/original/d53288db74aeb96ee3fef6f4d5608061.pdf e0d06171d63c913c86b6f8de3c0b0e20 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource DNA interference by a mesophilic Argonaute protein, CbcAgo [version 2; peer review: 2 approved] Creator An entity primarily responsible for making the resource Nieves García-Quintans, Laurie Bowden, José Berenguer, Mario Mencía Description An account of the resource Background: The search for putative enzymes that can facilitate gene editing has recently focused its attention on Argonaute proteins from prokaryotes (pAgos). Though they are structural homologues of human Argonaute protein, which uses RNA guides to interfere with RNA targets, pAgos use ssDNA guides to identify and, in many cases, cut a complementary DNA target. Thermophilic pAgos from Thermus thermophilus, Pyrococcus furiosus and Methanocaldococcus jasmanii have been identified and thoroughly studied, but their thermoactivity makes them of little use in mesophilic systems such as mammalian cells. Methods: Here we search for and identify CbcAgo, a prokaryotic Argonaute protein from a mesophilic bacterium, and characterize in vitro its DNA interference activity. Results: CbcAgo efficiently uses 5’P-ssDNA guides as small as 11-mers to cut ssDNA targets, requires divalent cations (preferentially, Mn2+) and has a maximum activity between 37 and 42 °C, remaining active up to 55 °C. Nicking activity on supercoiled dsDNA was shown. However, no efficient double-strand breaking activity could be demonstrated. Conclusions: CbcAgo can use gDNA guides as small as 11 nucleotides long to cut complementary ssDNA targets at 37ºC, making it a promising starting point for the development of new gene editing tools  for mammalian cells. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Identifier An unambiguous reference to the resource within a given context DOI: 10.12688/f1000research.18445.2 Source A related resource from which the described resource is derived F1000Research Publisher An entity responsible for making the resource available F1000 Research Ltd Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/ff89d913bc755ad30dffc47ae09cb89d.pdf af49e786495df5cc83cb20327b3c727f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Differential stepwise evolution of SARS coronavirus functional proteins in different host species Creator An entity primarily responsible for making the resource Tang Xianchun, Li Gang, Vasilakis Nikos, Zhang Yuan, Shizheng Li, Zhong Yang, Wang Lin-Fa, Zhang Shuyi Description An account of the resource Abstract Background SARS coronavirus (SARS-CoV) was identified as the etiological agent of SARS, and extensive investigations indicated that it originated from an animal source (probably bats) and was recently introduced into the human population via wildlife animals from wet markets in southern China. Previous studies revealed that the spike (S) protein of SARS had experienced adaptive evolution, but whether other functional proteins of SARS have undergone adaptive evolution is not known. Results We employed several methods to investigate selective pressure among different SARS-CoV groups representing different epidemic periods and hosts. Our results suggest that most functional proteins of SARS-CoV have experienced a stepwise adaptive evolutionary pathway. Similar to previous studies, the spike protein underwent strong positive selection in the early and middle phases, and became stabilized in the late phase. In addition, the replicase experienced positive selection only in human patients, whereas assembly proteins experienced positive selection mainly in the middle and late phases. No positive selection was found in any proteins of bat SARS-like-CoV. Furthermore, specific amino acid sites that may be the targets of positive selection in each group are identified. Conclusion This extensive evolutionary analysis revealed the stepwise evolution of different functional proteins of SARS-CoVs at different epidemic stages and different hosts. These results support the hypothesis that SARS-CoV originated from bats and that the spill over into civets and humans were more recent events. Date A point or period of time associated with an event in the lifecycle of the resource 2009 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1471-2148-9-52 Source A related resource from which the described resource is derived BMC Evolutionary Biology Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Evolution Language A language of the resource EN https://www.socictopen.socict.org/files/original/e3b99004e2099a72011b8f8cdd8ad2ba.pdf c29746f52db26a4cc06f08f0b1aa3d7a Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The Range of Application of Domestic Antiviral Drug in Рediatrics Creator An entity primarily responsible for making the resource O. V. Shamsheva, O. V. Molochkova Description An account of the resource The article presents the results of studies of the effectiveness and safety of domestic antiviral drug Аrbidol in children. Arbidol demonstrated antiviral activity not only against influenza viruses, and other respiratory viruses (respiratory syncytial virus, parainfluenza, rhinovirus), coronavirus, rotavirus, and others. Date A point or period of time associated with an event in the lifecycle of the resource 2015 Subject The topic of the resource antiviral drag, Respiratory viruses, acute respiratory infections (ARI), influenza, rotavirus infection, arbidol Identifier An unambiguous reference to the resource within a given context DOI: 10.22627/2072-8107-2015-14-4-26-30 Source A related resource from which the described resource is derived Detskie Infekcii (Moskva) Publisher An entity responsible for making the resource available LLC Diagnostics and Vaccines"" Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Pediatrics Language A language of the resource RU https://www.socictopen.socict.org/files/original/5f527677b8178cdcac68c44ac5082e95.pdf 42431bdb3ddccd4c664f3373054fe82b Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The Value of the Interferon Inductors in the Treatment and Prevention of Respiratory Infections Creator An entity primarily responsible for making the resource O.V. Kladova, O. V. Molochkova, N. L. Waltz, Yu. V. Kompaniets, N. L. Grishkevich Description An account of the resource The data on the causal effects of interferons (IFN) and interferon inductors on a wide range of respiratory viruses and stimulate the immune system. The mechanism of action of interferon inductors, provides an assessment of the efficacy and safety of domestic drug Amixin® (tiloron) for the treatment and prevention of acute respiratory viral infections and influenza in adults and children over 7 years. Application of Amixin® reduced the duration of the main symptoms of the disease contributed to the normalization of the immune status, reduced the incidence of SARS and influenza complications. Appointment of Amixin® in children older than 7 years with chronic herpes virus infection, has contributed to the clinical and laboratory remission and improvement of lymphocyte subpopulation composition. Date A point or period of time associated with an event in the lifecycle of the resource 2016 Subject The topic of the resource acute respiratory viral infections, influenza, interferons (ifn), interferon inductors, amixin® (tiloron), Children Identifier An unambiguous reference to the resource within a given context DOI: 10.22627/2072-8107-2016-15-4-48-53 Source A related resource from which the described resource is derived Detskie Infekcii (Moskva) Publisher An entity responsible for making the resource available LLC Diagnostics and Vaccines"" Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Pediatrics Language A language of the resource RU https://www.socictopen.socict.org/files/original/b49a34fee4db47c6977137c317266c95.pdf 5cda3248442ac2403983a5fb117af5bb Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Travel Medicine in Qatar: Strategic Action for Better Public Health Outcome Creator An entity primarily responsible for making the resource Mohamad Abdul Halim Chehab, Mohamed Bala, Ayman Al-Dahshan Description An account of the resource The 21st century has witnessed exponential growth in the travel industry as well as the emergence and re-emergence of several infectious diseases, such as the Middle East Respiratory Syndrome. Moreover, modern travel has eliminated spatial and temporal barriers, heightening the risk of global propagation of disease. Therefore, there is a need for balance between the health risks as well as the unique opportunities associated with travel, which is achievable through travel medicine.Despite the fact that pre-travel medical advice and post-travel medical care are vital for travelers’ health, many do not pursue such services and are unaware of the health risks associated with their destination.Primary care physicians, the travelers’ first line of contact with the heath system, must possess comprehensive knowledge of the dynamic epidemiology of travel-associated illnesses and the availability of new preventive or treatment measures. Most primary healthcare professionals portray a positive attitude towards giving travel-related health advice. However, such confidence must be fortified with appropriate knowledge and skills through certification programs, courses, or fellowships to build capacity and standardize the service provided. In the primary healthcare setting, the use of information technology in travel clinics has yielded good outcomes in terms of standardizing the care provided as well as guiding physicians and empowering their practice. However, the uptake of modern technologies does not eliminate the need for continuous professional development and health service evaluation Date A point or period of time associated with an event in the lifecycle of the resource 2018 Identifier An unambiguous reference to the resource within a given context DOI: 10.15171/IJTMGH.2018.24 Source A related resource from which the described resource is derived International Journal of Travel Medicine and Global Health Publisher An entity responsible for making the resource available International Travel Medicine Center of Iran Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/c4e5ad3cdad8f21559f5d2c4e25b6df3.pdf 292cf0764812645e35bc7d326c0f6041 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Novel Tetraplex Quantitative PCR Assays for Simultaneous Detection and Identification of Xylella fastidiosa Subspecies in Plant Tissues Creator An entity primarily responsible for making the resource Enora Dupas, Martial Briand, Marie-Agnès Jacques, Sophie Cesbron Description An account of the resource Xylella fastidiosa (Xf) is an insect-borne bacterium confined to the xylem vessels of plants. This plant pathogen has a broad host range estimated to 560 plant species. Five subspecies of the pathogen with different but overlapping host ranges have been described, but only three subspecies are widely accepted, namely subspecies fastidiosa, multiplex, and pauca. Initially limited to the Americas, Xf has been detected in Europe since 2013. As management of X. fastidiosa outbreaks in Europe depends on the identification of the subspecies, accurate determination of the subspecies in infected plants as early as possible is of major interest. Thus, we developed various tetraplex and triplex quantitative PCR (qPCR) assays for X. fastidiosa detection and subspecies identification in planta in a single reaction. We designed primers and probes using SkIf, a bioinformatics tool based on k-mers, to detect specific signatures of the species and subspecies from a data set of 58 genome sequences representative of X. fastidiosa diversity. We tested the qPCR assays on 39 target and 30 non-target strains, as well as on 13 different plant species spiked with strains of the different subspecies of X. fastidiosa, and on samples from various environmental and inoculated host plants. Sensitivity of simplex assays was equal or slightly better than the reference protocol on purified DNA. Tetraplex qPCR assays had the same sensitivity than the reference protocol and allowed X. fastidiosa detection in all spiked matrices up to 103 cells.ml−1. Moreover, mix infections of two to three subspecies could be detected in the same sample with tetraplex assays. In environmental plant samples, the tetraplex qPCR assays allowed subspecies identification when the current method based on multilocus sequence typing failed. The qPCR assays described here are robust and modular tools that are efficient for differentiating X. fastidiosa subspecies directly in plant samples. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource real-time PCR, TaqMan, subspecies differentiation, Diagnostic, SkIf, multiplexing Identifier An unambiguous reference to the resource within a given context DOI: 10.3389/fpls.2019.01732 Source A related resource from which the described resource is derived Frontiers in Plant Science Publisher An entity responsible for making the resource available Frontiers Media S.A. Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Plant culture Language A language of the resource EN