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                <text>The coronavirus disease 2019 (COVID-19) pandemic has highlighted various weaknesses in global healthcare services. The blood supply in Africa is a critical element of the healthcare service that may be significantly affected by the pandemic. By implementing principles of patient blood management, primary healthcare practitioners may play an important role in the resilience of the blood supply during the COVID-19 pandemic.</text>
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                <text>Introducción. En Cúcuta, Cryptococcus gattii de serotipo B se recupera comúnmente de pacientes inmunocompetentes con criptococosis, pero no ha sido recuperado del ambiente a pesar su alta incidencia, que es de 77 % de los casos reportados. Objetivo. El objetivo de este trabajo fue realizar un extenso muestreo ambiental en Cúcuta, para aislar C. gattii de serotipo B y expandir nuestro conocimiento sobre la ecología y la epidemiología de esta importante levadura. Materiales y métodos. Se recolectaron y se procesaron muestras asociadas con 3.634 árboles de 40 zonas de Cúcuta y 28 muestras cercanas a las casas de cuatro pacientes con criptococosis causada por C. gattii de serotipo B. El serotipo de los aislamientos recuperados se estableció usando PCR múltiple, el patrón molecular se determinó por RFLP del gen URA5 y la pareja sexual se determinó usando los iniciadores MfαU, MfαL, MFa2U y MFa2L. Resultados. Se procesaron 4.389 muestras y se recuperó un aislamiento de C. gattii de serotipo B (VGI/a), dos aislamientos de C. gattii de serotipo C (VGIII/α) y tres aislamientos de C. neoformans var. grubii de serotipo A (VNI/α). La densidad de los aislamientos recuperados varió entre 50 y 350 UFC/g de suelo. Conclusiones. Éste es el primer reporte de la recuperación ambiental de C. gattii de serotipo B en Cúcuta. Sin embargo, por la baja tasa de recuperación de aislamientos únicamente en suelo, el nicho ambiental de C. gattii no se pudo establecer, por lo que es necesario realizar posteriores estudios ambientales en Cúcuta, debido a que este serotipo, no sólo está causando criptococosis, sino también muestra una gran virulencia después del brote ocurrido en Vancouver.</text>
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                <text>2011</text>
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            </elementTextContainer>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>Colombia, Cryptococcus, Sampling studies, ecology, environment, mycoses</text>
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            <name>Identifier</name>
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                <text>10.7705/biomedica.v31i1.342</text>
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                <text>Biomédica: revista del Instituto Nacional de Salud</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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                <text>Instituto Nacional de Salud</text>
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                <text>Medicine, Arctic medicine. Tropical medicine</text>
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                <text>&lt;a href="http://www.revistabiomedica.org/index.php/biomedica/article/view/342" target="_blank" rel="noreferrer noopener"&gt;http://www.revistabiomedica.org/index.php/biomedica/article/view/342&lt;/a&gt;</text>
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              <name>Title</name>
              <description>A name given to the resource</description>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Primer design for complete sequencing of the Avastrovirus ORF2 gene</text>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="7550">
                <text>Luis Ramiro Luna Espinoza, Laila Andreia Rodrigues Beserra, Rodrigo Martins Soares, Fabio Gregori</text>
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            </elementTextContainer>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="7552">
                <text>2018</text>
              </elementText>
            </elementTextContainer>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="7553">
                <text>PCR, Sequencing, Avastrovirus, Avian, ORF2</text>
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            </elementTextContainer>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="7554">
                <text>DOI: 10.5433/1679-0359.2018v39n1p411</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="7555">
                <text>Semina: Ciências Agrárias</text>
              </elementText>
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          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="7556">
                <text>Universidade Estadual de Londrina</text>
              </elementText>
            </elementTextContainer>
          </element>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
            <elementTextContainer>
              <elementText elementTextId="7557">
                <text>Agriculture (General)</text>
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            <name>Language</name>
            <description>A language of the resource</description>
            <elementTextContainer>
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                <text>EN, PT</text>
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          <name>Dublin Core</name>
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              <name>Title</name>
              <description>A name given to the resource</description>
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                <elementText elementTextId="1">
                  <text>Coronavirus</text>
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              </elementTextContainer>
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            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="2">
                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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      <elementSet elementSetId="1">
        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
            <elementTextContainer>
              <elementText elementTextId="21112">
                <text>Primer fabrication using polymerase mediated oligonucleotide synthesis</text>
              </elementText>
            </elementTextContainer>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="21113">
                <text>Thomas Torsten, Cairns Murray J, Beltran Carolina E, Tillett Daniel</text>
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            </elementTextContainer>
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            <name>Description</name>
            <description>An account of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="21114">
                <text>Abstract Background Custom solid phase oligonucleotide synthesis is an important foundation supporting nearly every aspect of current genomics. In spite of the demand for oligonucleotide primers, their synthesis remains relatively expensive, time consuming and in many circumstances a wasteful process. In this methodology, described as polymerase mediated oligonucleotide synthesis (PMOS), a DNA polymerase is used to increase the hybridization affinity of one oligonucleotide by using another as a template for DNA synthesis. This self-assembly process provides an opportunity to instantly generate a very large number of useful gene-specific primers from a small library of simple precursors. PMOS can be used to generate primers directly in the end-users laboratory within the context of any DNA polymerase chemistry such as in PCR or sequencing reactions Results To demonstrate the utility of PMOS, a universal 768-member oligonucleotide library (UniSeq) was designed, fabricated and its performance optimized and evaluated in a range of PCR and DNA sequencing reactions. This methodology used to derive specific 11-mers, performed well in each of these activities and produced the desired amplification or sequencing analysis with results comparable to primers made by time consuming and expensive custom synthesis. Conclusion On the basis of these experiments, we believe this novel system would be broadly applicable and could in many circumstances replace the need for conventional oligonucleotide synthesis.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="21115">
                <text>2009</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="43">
            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
            <elementTextContainer>
              <elementText elementTextId="21116">
                <text>DOI: 10.1186/1471-2164-10-344</text>
              </elementText>
            </elementTextContainer>
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          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="21117">
                <text>BMC Genomics</text>
              </elementText>
            </elementTextContainer>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="21118">
                <text>BMC</text>
              </elementText>
            </elementTextContainer>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="21119">
                <text>Genetics, Biotechnology</text>
              </elementText>
            </elementTextContainer>
          </element>
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            <name>Language</name>
            <description>A language of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="21120">
                <text>EN</text>
              </elementText>
            </elementTextContainer>
          </element>
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    <fileContainer>
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        <src>https://www.socictopen.socict.org/files/original/d1e0e51457a5c864831e3a4797eb9b4e.pdf</src>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
              <elementTextContainer>
                <elementText elementTextId="88121">
                  <text>Agricultura sostenible</text>
                </elementText>
              </elementTextContainer>
            </element>
            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="88122">
                  <text>Dominio científico: Agricultura sostenible</text>
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              </elementTextContainer>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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        <name>Dublin Core</name>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Primer hallazgo del Virus de la Parálisis Aguda Israelí (IAPV) en Nezara viridula (Hemiptera: Pentatomidae)</text>
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            </elementTextContainer>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="183641">
                <text>María L. SUSEVICH, Francisco J. REYNALDI, Gerardo A. MARTI, María G. ECHEVERRÍA</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="183642">
                <text>Se llevó a cabo un relevamiento de virus patógenos de abejas en ejemplares de chinche verde Nezara viridula (Hemiptera: Pentatomidae) en el Cinturón  Hortícola Platense (CHP). Se detectó la presencia del Virus israelí de la parálisis aguda  (IAPV) mediante técnicas moleculares. Estos resultados permitirían inferir que las infecciones virales en los insectos son de gran importancia ya que podrían actuar como reservorios virales, información que podría ser útil para conocer la dinámica del virus en la naturaleza. Este trabajo detecta por primera vez la presencia de IAPV en N. viridula. Serán necesarios estudios adicionales para entender la ecología de estos virus en las comunidades de artrópodos del CHP y determinar el rol que cumplen las chinches como posibles reservorios de estos virus en el ambiente.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2019</text>
              </elementText>
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          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="183644">
                <text>Revista de la Sociedad Entomológica Argentina</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="183645">
                <text>Sociedad Entomológica Argentina</text>
              </elementText>
            </elementTextContainer>
          </element>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Zoology</text>
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          </element>
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            <name>Relation</name>
            <description>A related resource</description>
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                <text>&lt;a href="http://www.redalyc.org/articulo.oa?id=322057512001" target="_blank" rel="noreferrer noopener"&gt;http://www.redalyc.org/articulo.oa?id=322057512001&lt;/a&gt;</text>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Agricultura sostenible</text>
                </elementText>
              </elementTextContainer>
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              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Primer registro de la familia Morchellaceae (Ascomycota: Pezizales) para Colombia</text>
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          </element>
          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="218240">
                <text>César Augusto Pinzón Osorio, Jonás Pinzón Osorio</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="218241">
                <text>Se registra por primera vez al género Morchella Dill. ex Pers. (Ascomycota, Pezizales), único representante de la familia Morchellaceae para la micobiota Colombiana. El ascocarpo fue recolectado en un bosque muy húmedo premontano, en la vereda Santa Helena, municipio de Gachalá, departamento de Cundinamarca. El espécimen es descrito e ilustrado con fotografías y se aporta información sobre su distribución, ecología y sustrato de crecimiento. La identidad taxonómica del ejemplar es discutida de acuerdo al concepto morfológico tradicional establecido en la literatura micológica. Con este reporte, en Colombia el orden Pezizales queda constituido por 17 especies contenidas en 12 géneros y 7 familias.</text>
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          <element elementId="40">
            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="218242">
                <text>2017</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="218243">
                <text>Ascomycota, Colombia, Morchella, Pezizales, nuevo registro</text>
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            </elementTextContainer>
          </element>
          <element elementId="43">
            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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                  <text>Agricultura sostenible</text>
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                <text>Primer registro de Laubieriopsis brevis (Hartman, 1965) para la fauna de la península Ibérica</text>
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                <text>En el transcurso de un programa de investigación realizado el 9 de junio de 2007, se recogieron tres ejemplares de la especie Laubieriopsis brevis (Hartman, 1965); primera ocasión en que se tiene constancia de este poliqueto para la fauna de la península Ibérica. Los ejemplares fueron recogidos en un fondo fangoso del margen superior del talud continental de Gipuzkoa a 475 m de profundidad. Con este registro, la familia Fauveliopsidae queda representada en la península Ibérica por los dos géneros que la componen: Fauveliosis McIntosh, 1922 y Laubieriopsis Petersen, 2000. En el presente artículo se describen aspectos relativos a su morfologia, ecología, hábitat y distribución geográfica.</text>
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                <text>Primer registro; Laubieriopsis brevis; Annelida; Fauveliopsidae; península Ibérica</text>
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                <text>Munibe Ciencias Naturales</text>
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                <text>Sociedad de Ciencias Aranzadi</text>
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                <text>&lt;a href="http://www.aranzadi.eus/fileadmin/docs/Munibe/2012039050CN.pdf" target="_blank" rel="noreferrer noopener"&gt;http://www.aranzadi.eus/fileadmin/docs/Munibe/2012039050CN.pdf&lt;/a&gt;</text>
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