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                  <text>Dominio científico: Coronavirus</text>
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                <text>Re-analysis of Single Cell Transcriptome Reveals That the NR3C1-CXCL8-Neutrophil Axis Determines the Severity of COVID-19</text>
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                <text>Heung Kyu Lee, Jang Hyun Park, Heung Kyu Lee</text>
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                <text>SARS-CoV-2 infection has recently been declared a pandemic. Some patients showing severe symptoms exhibit drastic inflammation and airway damage. In this study, we re-analyzed published scRNA-seq data of COVID-19 patient bronchoalveolar lavage fluid to further classify and compare immunological features according to the patient’s disease severity. Patients with severe symptoms showed DNA damage and apoptotic features of epithelial cells. Our results suggested that epithelial damage was associated with neutrophil infiltration. Myeloid cells of severe patients showed higher expression of proinflammatory cytokines and chemokines such as CXCL8. As a result, neutrophils were abundant in lungs of patients from the severe group. Furthermore, recruited neutrophils highly expressed genes related to neutrophil extracellular traps. Neutrophil-mediated inflammation was regulated by glucocorticoid receptor expression and activity. Based on these results, we suggest that severe COVID-19 symptoms may be determined by differential expression of glucocorticoid receptors and neutrophils.</text>
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                <text>covid-19, SARS-CoV-2, neutrophil, glucocorticoid, BAL, CXCL8</text>
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                <text>10.3389/fimmu.2020.02145</text>
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                <text>Epidemiology and Health</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>Immunologic diseases. Allergy</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Re-description of Daphnia (Ctenodaphnia) from lakes in the Khumbu Region, Nepalese Himalayas, with the erection of a new species, Daphnia himalaya, and a note on an intersex individual</text>
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                <text>Dinora Carolina PEÑALVA-ARANA, John A.H. BENZIE, Patrick Martin, Marina Manca</text>
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                <text>We present here a detailed description of Ctenodaphnia-type Daphnia specimens collected from lakes in the Nepalese Himalayas between 1994 and 2004, including mature females and adult males. The specimens examined share certain diagnostic traits with Daphnia tibetana (Sars 1903), and others with Daphnia fusca (Gurney 1906). A re-appraisal of their previous synonymy with D. fusca and a comparison with all published descriptions of similar species from the region indicate that they represent a new species named here as Daphnia himalaya. The chance discovery of a sex intergrade of the same species allowed a description of the secondary sexual characteristics in this interesting and rare intermediate state to be reported, and its possible ecological implications to be discussed, as well.</text>
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                <text>2006</text>
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                <text>Cladocera, Daphnia, taxonomy, Himalayas, sex intergrade</text>
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                <text>DOI: 10.4081/jlimnol.2006.132</text>
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                <text>Journal of Limnology</text>
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                <text>PAGEPress Publications</text>
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                <text>Environmental sciences, Geography. Anthropology. Recreation, Physical geography</text>
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                <text>Re-emerging Middle East respiratory syndrome coronavirus: The hibernating bat hypothesis</text>
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                <text>Hana Fakhoury, Ali Hajeer</text>
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            <name>Identifier</name>
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                <text>DOI: 10.4103/1817-1737.160847</text>
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                <text>Annals of Thoracic Medicine</text>
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                <text>Wolters Kluwer Medknow Publications</text>
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                <text>Diseases of the circulatory (Cardiovascular) system, Diseases of the respiratory system</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Re-infection with SARS-CoV-2 in Patients Undergoing Serial Laboratory Testing.</text>
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                <text>Adnan I Qureshi, William I Baskett, Wei Huang, Iryna Lobanova, S Hasan Naqvi, Chi-Ren Shyu</text>
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                <text>A better understanding of re-infection after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has become one of the healthcare priorities in the current pandemic. We determined the rate of re-infection, associated factors and mortality during follow up in a cohort of patients with SARS-CoV-2 infection. We analyzed 9,119 patients with SARS-CoV-2 infection who received serial tests in total of 62 healthcare facilities in United States between December 1, 2019 to November 13, 2020. Re-infection was defined by two positive tests separated by interval of greater than 90 days two after resolution of first infection was confirmed by two or more consecutive negative tests. We performed logistic regression analysis to identify demographic and clinical characteristics associated with re-infection. Re-infection was identified in 0.7% (n=63, 95% confidence interval [CI] 0.5%-0.9%) during follow up of 9,119 patients with SARS-CoV-2 infection. The mean period (±standard deviation [SD]) between two positive tests was 116 ± 21 days. A logistic regression analysis identified that asthma (odds ratio [OR] 1.9, 95% CI 1.1-3.2) and nicotine dependence/tobacco use (OR 2.7, 95% CI 1.6-4.5) were associated with re-infection. There was a significantly lower rate of pneumonia, heart failure, and acute kidney injury observed with re-infection compared with primary infection among the 63 patients with re-infection There were two deaths (3.2%) associated with re-infection. We identified a low rate of re-infection confirmed by laboratory tests in a large cohort of patients with SARS-CoV-2 infection. Although re-infection appeared to be milder than primary infection, there was associated mortality.</text>
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                <text>2021</text>
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                <text>coronavirus, covid-19, SARS-CoV-2, Reinfection, Laboratory tests</text>
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                <text>10.1093/cid/ciab345</text>
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                <text>Clinical infectious diseases : an official publication of the Infectious Diseases Society of America</text>
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                <text>Re-positive Cases of Nucleic Acid Tests in Discharged Patients With COVID-19: A Follow-Up Study</text>
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                <text>Xiao Wang, Xiaofeng Xu, Xiaoying Liu, Xiang Du, Lisha Luo, Bing-Hui Li, Hao Zi, Guoxiong Liu, Xi-Min Qiao, Zhi-Hai Tian</text>
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                <text>Background: The frequent emergence of the re-positive patients with COVID-19 is a potential threat worldwide. This study aimed to describe data from admission to follow-up for patients with COVID-19 and analyze the possible causes for re-positive nucleic acid tests to provide more scientific basis for reducing the numbers of re-positive patients after discharge.Methods: We retrospectively recorded 15 patients with COVID-19 admitted to the Xianyang Central Hospital, China. The baseline, exposure histories, clinical syndromes, laboratory characteristics, nucleic acid, and follow-up tests were analyzed, and the radiological characteristics of re-positive patient at different periods were compared.Results: Eight (53.33%) patients had the history of travel to Wuhan, four (26.67%) patients had close contact with confirmed patients, and one (6.67%) patient had close contact with suspected patients. After treatment, all patients had two consecutively negative nucleic acid tests and were discharged from hospital. All patients were followed up for more than 14 days, and the average time from discharge to the first follow-up was 14.67 ± 3.31 days (from 9 to 22 days). Most patients showed no clinical symptoms and negative nucleic acid tests, while one patient had an itchy throat, her CT scan showed a light density shadow in the right lower lobe of the lung, and the nucleic acid was once again positive. The second follow-up of the other 14 patients (except the re-positive one) was conducted 20.80 ± 7.78 days (from 13 to 30 days) after discharge, and all of them had negative nucleic acid tests. The positive patient was immediately readmitted and received a new round of treatment. Her family members and colleagues remained healthy until now.Conclusions: The quality of nucleic acid testing reagents should be enhanced, and the training of nucleic acid sampling operators should be strengthened to reduce the false-negative results in the nucleic acid of SARS-CoV-2; the clinical specimens of throat and nasopharynx swabs can be collected at the same time; IgM- and IgG-specific antibodies of SARS-CoV-2 should be carried out for discharged patients; the radiological characteristics should be evaluated strictly; and the discharge standard can be specified according to the baseline and severity of disease of patients.</text>
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                <text>DOI: 10.3389/fmed.2020.00349</text>
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