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                <text>Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus</text>
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                <text>Jonas M. Winchell, Paul A. Rota, Bettina Bankamp, Larry J. Anderson, Michael D. Bowen, Dean D. Erdman, Suxiang Tong, William J. Bellini, Richard F. Meyer, Shannon L. Emery, Bruce R. Newton, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Luis E. Lowe, Tom G. Ksiazek</text>
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                <text>A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of</text>
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                <text>Canada, real-time PCR, RT-PCR, coronavirus, Thailand, SARS</text>
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                <text>DOI: 10.3201/eid1002.030759</text>
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                <text>Emerging Infectious Diseases</text>
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                <text>Real-time sequence-validated loop-mediated isothermal amplification assays for detection of Middle East respiratory syndrome coronavirus (MERS-CoV).</text>
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                <text>Sanchita Bhadra, Yu Sherry Jiang, Mia R. Kumar, Reed  F. Johnson, Lisa E. Hensley, Andrew D. Ellington</text>
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                <text>The Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging human coronavirus, causes severe acute respiratory illness with a 35% mortality rate. In light of the recent surge in reported infections we have developed asymmetric five-primer reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for detection of MERS-CoV. Isothermal amplification assays will facilitate the development of portable point-of-care diagnostics that are crucial for management of emerging infections. The RT-LAMP assays are designed to amplify MERS-CoV genomic loci located within the open reading frame (ORF)1a and ORF1b genes and upstream of the E gene. Additionally we applied one-step strand displacement probes (OSD) for real-time sequence-specific verification of LAMP amplicons. Asymmetric amplification effected by incorporating a single loop primer in each assay accelerated the time-to-result of the OSD-RT-LAMP assays. The resulting assays could detect 0.02 to 0.2 plaque forming units (PFU) (5 to 50 PFU/ml) of MERS-CoV in infected cell culture supernatants within 30 to 50 min and did not cross-react with common human respiratory pathogens.</text>
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                <text>DOI: 10.1371/journal.pone.0123126</text>
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                <text>Realidade territorial de unidades familiares no semiárido brasileiro</text>
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                <text>RESUMO O crescente interesse em dinâmica da (re)produção familiar advém sua importância para o desenvolvimento do setor agrário brasileiro. Para a compreensão da dinâmica do território familiar no semiárido brasileiro o estudo englobou 96 unidades familiares (ufs) de seis comunidades rurais (Tauá-ce). Variáveis e análises que apreendessem atributos da dinâmica socioeconômica das ufs como reflexo da capacidade dos atores sintagmáticos de transformar a natureza por meio de relações produtivas foram realizadas. Identificaram-se grupos de imagens territoriais que possuem sistemas de relações produtivas diferentes e que viabilizam a territorialidade.</text>
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                <text>Universidade de São Paulo</text>
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                <text>&lt;a href="http://www.scielo.br/scielo.php?script=sci_arttext&amp;amp;pid=S0103-20702016000100055&amp;amp;lng=en&amp;amp;tlng=en" target="_blank" rel="noreferrer noopener"&gt;http://www.scielo.br/scielo.php?script=sci_arttext&amp;amp;pid=S0103-20702016000100055&amp;amp;lng=en&amp;amp;tlng=en&lt;/a&gt;</text>
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                <text>Reality vs. Propaganda. PTSD among Civilian Healthcare Staff and Patients and the Rhetorical Invention of the War on Coronavirus""</text>
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                <text>Patrizia Piredda</text>
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                <text>As the coronavirus pandemic broke out in March of 2020, many countries’ healthcare systems were overwhelmed by the sudden increase in the number of hospitalised patients and deaths. Not only hospitals but the healthcare staff struggled too, who faced unprecedented stressful work conditions for which they often had no training. Politicians and the media addressed the sanitary crisis using the metaphor of ‚war on coronavirus‛, calling the national and international communities to an emotional ‚call to arms‛. However, the article argues that the use of war metaphors was misleading and incorrect because PTSD triggered in civilian healthcare staff by the harsh work conditions during the crisis was not entirely comparable with PTSD in military medical staff deployed in combat zones. The ethical burden and the exposure to risks of physical injury and death made the conditions of these two groups comparable. However, the technical and professional preparation of the military and the civilian healthcare staff diverge and make their operational contexts incomparable. The author thus argues that the ‚war on coronavirus‛ was a wrong metaphor used to divert public attention from the actual state of the inefficiency of the national health systems in such countries as Italy and the UK.</text>
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                <text>coronavirus, Metaphor, ptsd in civilian healthcare staff, rhetorical manipulation and propaganda</text>
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                <text>Epidemiology and Health</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>La descripción de fortalezas y debilidades en la evolución de las normas del ordenamiento territorial en Colombia, el análisis crítico de los determinantes en la experiencia de formulación y ejecución de los Planes de Ordenamiento Territorial (POT), y la explicación de cuáles de ellos deben ser directrices prioritarias para orientar un nuevo proceso de formulación de esos instrumentos, develan que, si bien la gestión del riesgo de desastres naturales es una directriz para orientar el proceso, en ella pervive un desequilibrio jurídico e institucional en la correlación que debe tener con el ordenamiento territorial, los reasentamientos y la reubicación. Desde una investigación documental sustentada en el modelo de investigación cualitativo y el enfoque dogmático jurídico, este escrito desarrolla las razones para regular la materia en un país abrumado por la sobreproducción de normas, toda vez que la ausencia de legislación mantiene un vacío jurídico del que se desprende la amenaza y la vulneración de derechos humanos. Para ello, la necesidad de legislar los procesos de reasentamiento y reubicación poblacional generados por eventos naturales se sustenta a partir de los compromisos adquiridos por Colombia mediante el reconocimiento de instrumentos internacionales, de algunos precedentes jurisprudenciales que determinan el alcance de una iniciativa legislativa, de las principales disposiciones nacionales que contribuyen a desarrollar legislativamente los procesos de reasentamiento y reubicación, y de algunas falencias de políticas nacionales recientes sobre cambio climático y la gestión del riesgo que dan cuenta de la urgencia de promover legislativamente garantías y procedimientos que incidan en su modificación, al no contemplar el reasentamiento y la reubicación poblacional.</text>
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                <text>Universidad del Magdalena</text>
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                <text>&lt;a href="http://revistas.unimagdalena.edu.co/index.php/jangwapana/article/view/2928" target="_blank" rel="noreferrer noopener"&gt;http://revistas.unimagdalena.edu.co/index.php/jangwapana/article/view/2928&lt;/a&gt;</text>
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                <text>Recensión: Marta Torre-Schaub (Dir.), Droit et changement climatique : comment répondre à l’urgence climatique ? Regards croisés à l’interdisciplinaire, Collection de l’Institut des Sciences Juridique et Philosophique de la Sorbonne, Ed. mare &amp; martin, París, 2020, 302 pp.</text>
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                <text>Blanca Soro Mateo</text>
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                <text>Editum, Ediciones de la Universidad de Murcia</text>
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                <text>Recent Advancements in the Diagnosis, Prevention, and Prospective Drug Therapy of COVID-19</text>
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                <text>Waquar Ahsan, Mohammed Al-Bratty, Hassan A. Alhazmi, Hassan A. Alhazmi, Kuldeep Singh Patel, Bharti Mangla, Shamama Javed, Asim Najmi, Muhammad Hadi Sultan, Hafiz A. Makeen, Asaad Khalid, Syam Mohan, Manal M. E. Taha, Shahnaz Sultana</text>
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                <text>Severe acute respiratory syndrome coronavirus (CoV)-2 (SARS-CoV-2), previously called 2019 novel CoV, emerged from China in late December 2019. This virus causes CoV disease-19 (COVID-19), which has been proven a global pandemic leading to a major outbreak. As of June 19, 2020, the data from the World Health Organization (WHO) showed more than 8.7 million confirmed cases in over 200 countries/regions. The WHO has declared COVID-19 as the sixth public health emergency of international concern on January 30, 2020. CoVs cause illnesses that range in severity from the common cold to severe respiratory illnesses and death. Nevertheless, with technological advances and imperative lessons gained from prior outbreaks, humankind is better outfitted to deal with the latest emerging group of CoVs. Studies on the development of in vitro diagnostic tests, vaccines, and drug re-purposing are being carried out in this field. Currently, no approved treatment is available for SARS-CoV-2 given the lack of evidence. The results from preliminary clinical trials have been mixed as far as improvement in the clinical condition and reduction in the duration of treatment are concerned. A number of new clinical trials are currently in progress to test the efficacy and safety of various approved drugs. This review focuses on recent advancements in the field of development of diagnostic tests, vaccines, and treatment approaches for COVID-19.</text>
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                <text>coronavirus, vaccines, 2019ncov, covid-19, Diagnosis, SARS-CoV-2</text>
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              <elementText elementTextId="85603">
                <text>10.3389/fpubh.2020.00384</text>
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                <text>Epidemiology and Health</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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                <text>Korean Society of Epidemiology</text>
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            </elementTextContainer>
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              <elementText elementTextId="85606">
                <text>Public aspects of medicine</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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                <text>Recent Advances in AIV Biosensors Composed of Nanobio Hybrid Material</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="21075">
                <text>Taek Lee, Jae-Hyuk Ahn, Sun  Yong Park, Ga-Hyeon Kim, Jeonghyun Kim, Tae Hyung Kim, Inho Nam, Chul Hwan Park, Min-Ho Lee</text>
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            <description>An account of the resource</description>
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                <text>Since the beginning of the 2000s, globalization has accelerated because of the development of transportation systems that allow for human and material exchanges throughout the world. However, this globalization has brought with it the rise of various pathogenic viral agents, such as Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), Zika virus, and Dengue virus. In particular, avian influenza virus (AIV) is highly infectious and causes economic, health, ethnical, and social problems to human beings, which has necessitated the development of an ultrasensitive and selective rapid-detection system of AIV. To prevent the damage associated with the spread of AIV, early detection and adequate treatment of AIV is key. There are traditional techniques that have been used to detect AIV in chickens, ducks, humans, and other living organisms. However, the development of a technique that allows for the more rapid diagnosis of AIV is still necessary. To achieve this goal, the present article reviews the use of an AIV biosensor employing nanobio hybrid materials to enhance the sensitivity and selectivity of the technique while also reducing the detection time and high-throughput process time. This review mainly focused on four techniques: the electrochemical detection system, electrical detection method, optical detection methods based on localized surface plasmon resonance, and fluorescence.</text>
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                <text>2018</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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              <elementText elementTextId="21078">
                <text>avian influenza virus biosensor, Avian Influenza Virus, electrochemical detection, electrical detection, localized surface plasmon resonance, fluorescence, nanobio hybrid materials</text>
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            </elementTextContainer>
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                <text>DOI: 10.3390/mi9120651</text>
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            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="21080">
                <text>Micromachines</text>
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            </elementTextContainer>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="21081">
                <text>MDPI AG</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Mechanical engineering and machinery</text>
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            <description>A language of the resource</description>
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              <elementText elementTextId="21083">
                <text>EN</text>
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            <element elementId="50">
              <name>Title</name>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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                <text>Recent Advances in the Vaccine Development Against Middle East Respiratory Syndrome-Coronavirus</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="20098">
                <text>Chean Yeah Yong, Hui Kian Ong, Swee Keong Yeap, Kok Lian Ho, Wen Siang Tan</text>
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            <description>An account of the resource</description>
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                <text>Middle East respiratory syndrome (MERS) is a deadly viral respiratory disease caused by MERS-coronavirus (MERS-CoV) infection. To date, there is no specific treatment proven effective against this viral disease. In addition, no vaccine has been licensed to prevent MERS-CoV infection thus far. Therefore, our current review focuses on the most recent studies in search of an effective MERS vaccine. Overall, vaccine candidates against MERS-CoV are mainly based upon the viral spike (S) protein, due to its vital role in the viral infectivity, although several studies focused on other viral proteins such as the nucleocapsid (N) protein, envelope (E) protein, and non-structural protein 16 (NSP16) have also been reported. In general, the potential vaccine candidates can be classified into six types: viral vector-based vaccine, DNA vaccine, subunit vaccine, nanoparticle-based vaccine, inactivated-whole virus vaccine and live-attenuated vaccine, which are discussed in detail. Besides, the immune responses and potential antibody dependent enhancement of MERS-CoV infection are extensively reviewed. In addition, animal models used to study MERS-CoV and evaluate the vaccine candidates are discussed intensively.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2019</text>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
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                <text>Sinosh Skariyachan, Sneha Basavaraj Challapilli, Swathi Packirisamy, Supreetha Toplar Kumargowda, Vaishnavi Sneha Sridhar</text>
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                <text>Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is an emerging zoonotic virus considered as one of the major public threat with a total number of 2 298 laboratory-confirmed cases and 811 associated deaths reported by World Health Organization as of January 2019. The transmission of the virus was expected to be from the camels found in Middle Eastern countries via the animal and human interaction. The genome structure provided information about the pathogenicity and associated virulent factors present in the virus. Recent studies suggested that there were limited insight available on the development of novel therapeutic strategies to induce immunity against the virus. The severities of MERS-CoV infection highlight the necessity of effective approaches for the development of various therapeutic remedies. Thus, the present review comprehensively and critically illustrates the recent aspects on the epidemiology of the virus, the structural and functional features of the viral genome, viral entry and transmission, major mechanisms of pathogenesis and associated virulent factors, current animal models, detection methods and novel strategies for the development of vaccines against MERS-CoV. The review further illustrates the molecular and computational virtual screening platforms which provide insights for the identification of putative drug targets and novel lead molecules toward the development of therapeutic remedies.</text>
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                <text>Frontiers in Microbiology</text>
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                <text>Frontiers Media S.A.</text>
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                <text>Microbiology</text>
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