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                  <text>Dominio científico: Coronavirus</text>
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                <text>Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections</text>
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                <text>Müller Marcel A, Bae Hi-Gung, Thulke Stefanie, Radonić Aleksandar, Siegert Wolfgang, Nitsche Andreas</text>
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                <text>Abstract Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</text>
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                <text>2005</text>
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                <text>DOI: 10.1186/1743-422X-2-7</text>
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                <text>Virology Journal</text>
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                <text>Infectious and parasitic diseases</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Reference-free validation of short read data.</text>
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                <text>Jan Schröder, James Bailey, Thomas Conway, Justin Zobel</text>
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                <text>BACKGROUND: High-throughput DNA sequencing techniques offer the ability to rapidly and cheaply sequence material such as whole genomes. However, the short-read data produced by these techniques can be biased or compromised at several stages in the sequencing process; the sources and properties of some of these biases are not always known. Accurate assessment of bias is required for experimental quality control, genome assembly, and interpretation of coverage results. An additional challenge is that, for new genomes or material from an unidentified source, there may be no reference available against which the reads can be checked. RESULTS: We propose analytical methods for identifying biases in a collection of short reads, without recourse to a reference. These, in conjunction with existing approaches, comprise a methodology that can be used to quantify the quality of a set of reads. Our methods involve use of three different measures: analysis of base calls; analysis of k-mers; and analysis of distributions of k-mers. We apply our methodology to wide range of short read data and show that, surprisingly, strong biases appear to be present. These include gross overrepresentation of some poly-base sequences, per-position biases towards some bases, and apparent preferences for some starting positions over others. CONCLUSIONS: The existence of biases in short read data is known, but they appear to be greater and more diverse than identified in previous literature. Statistical analysis of a set of short reads can help identify issues prior to assembly or resequencing, and should help guide chemical or statistical methods for bias rectification.</text>
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                <text>DOI: 10.1371/journal.pone.0012681</text>
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                <text>PLoS ONE</text>
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                <text>Reflections on a Patient Suicide in the Time of COVID-19.</text>
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                <text>Yi Yen Annie Yeh</text>
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                <text>Academic psychiatry : the journal of the American Association of Directors of Psychiatric Residency Training and the Association for Academic Psychiatry</text>
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                  <text>Agricultura sostenible</text>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>REFLECTIONS ON DAIRY PRODUCTION IN THE WESTERN REGION OF SANTA CATARINA: COOPERATIVE IMPACTS FOR REGIONAL DEVELOPMENT</text>
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                <text>Natália Carrão Winckler, Gisele Trindade Molinari</text>
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                <text>This article reflects on the historical evolution of dairy production in Western of Santa Catarina in recent decades. The focus of this work lies on different influences over the configuration of this productive sector, including cooperativism and its nuances. To support the discussion, we interviewed seven experts and investigated how occurred insertion and changes in dairy farming over the years and how it has contributed to regional development. The analysis shows that, given the demand for expertise and high investment in new production technologies in poultry and swine production, which would be potential exclusion criteria for less capitalized farmers, the dairy farming has enabled many of these to remain in the countryside. On the one hand, institutional, legal and market factors have been pushing on poultry and swine producers and dairy farmers for production technology changes. On the other hand, this also influences on the strategies of dairy farmers for maintaining their activities, counting with the support of associations and cooperatives oriented to the development of the region..</text>
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                <text>Cooperação. Cadeia Produtiva do Leite. Agricultura familiar</text>
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                <text>Universidade de Santa Cruz do Sul</text>
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                <text>Social Sciences, Social sciences (General), Human ecology. Anthropogeography</text>
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                <text>&lt;a href="https://online.unisc.br/seer/index.php/redes/article/view/5298" target="_blank" rel="noreferrer noopener"&gt;https://online.unisc.br/seer/index.php/redes/article/view/5298&lt;/a&gt;</text>
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                <text>Reflections on Practice during a Pandemic: How do we Continue to Ensure Effective Communication during the COVID-19 Pandemic?</text>
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                <text>Wendy Roberts</text>
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                <text>10.1002/car.2660</text>
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                <text>Child abuse review (Chichester, England : 1992)</text>
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