202 10 20655 https://www.socictopen.socict.org/files/original/c38e63525a402242aec9b58e9c261540.pdf 39d3815d1660a901475ff1de63b5cd82 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane protein Creator An entity primarily responsible for making the resource Suliman Tasnim, Kallies Stephan, Schulz Axel R, Lehmann Dörte, Bader Oliver, Voß Daniel, van der Hoek Lia, Müller Marcel A, Fielding Burtram C, Drosten Christian, Niedrig Matthias Description An account of the resource Abstract Background Human pathogenic coronavirus NL63 (hCoV-NL63) is a group 1 (alpha) coronavirus commonly associated with respiratory tract infections. In addition to known non-structural and structural proteins all coronaviruses have one or more accessory proteins whose functions are mostly unknown. Our study focuses on hCoV-NL63 open reading frame 3 (ORF 3) which is a highly conserved accessory protein among coronaviruses. Results In-silico analysis of the 225 amino acid sequence of hCoV-NL63 ORF 3 predicted a triple membrane-spanning protein. Expression in infected CaCo-2 and LLC-MK2 cells was confirmed by immunofluorescence and Western blot analysis. The protein was detected within the endoplasmatic reticulum/Golgi intermediate compartment (ERGIC) where coronavirus assembly and budding takes place. Subcellular localization studies using recombinant ORF 3 protein transfected in Huh-7 cells revealed occurrence in ERGIC, Golgi- and lysosomal compartments. By fluorescence microscopy of differently tagged envelope (E), membrane (M) and nucleocapsid (N) proteins it was shown that ORF 3 protein colocalizes extensively with E and M within the ERGIC. Using N-terminally FLAG-tagged ORF 3 protein and an antiserum specific to the C-terminus we verified the proposed topology of an extracellular N-terminus and a cytosolic C-terminus. By in-vitro translation analysis and subsequent endoglycosidase H digestion we showed that ORF 3 protein is N-glycosylated at the N-terminus. Analysis of purified viral particles revealed that ORF 3 protein is incorporated into virions and is therefore an additional structural protein. Conclusions This study is the first extensive expression analysis of a group 1 hCoV-ORF 3 protein. We give evidence that ORF 3 protein is a structural N-glycosylated and virion-incorporated protein. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1743-422X-7-6 Source A related resource from which the described resource is derived Virology Journal Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/7035badf17f08a27e094dfe569c4b772.pdf 5c5e8044acaa1839e0242cd2e2e492a1 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Taking the first step is to move half way Creator An entity primarily responsible for making the resource Loza-Murguia Manuel G. Description An account of the resource Journal of the Selva Andina Research Society (JSARS), is an official Journal of the Society for Researching Selva Andina (Selva Andina Research Society “SARS”) of the Department of Education and Research in Biochemistry & Microbiology (in Spanish DEI & BM), the Peasant Academic Unit from Carmen Pampa of the Bolivian Catholic University “San Pablo”, from the year 2009, is being part of the net SciELO Scientific Electronic Library Online in developing.The net of electronic publication of journals by internet, response to the requirements of the countries from Latina America and Caribbean, as a support to the scientific communication, providing visibility and access to scientific literature, its respects the common methodology in the preparation, storage, dissemination and evaluation of scientific articles in electronic format. To date, eight Bolivian journals are being part of the SciELO Network - Bolivia: http:www.scielo.org.Participating countries such as Argentina, Brazil, Chile, Colombia, Cuba, Spain, Portugal and Venezuela, besides SciELO “SciELO Public Health and Social Sciences”; countries that are developing as Bolivia, Mexico, Paraguay, Costa Rica, Peru, Uruguay and South Africa .SciELO - Bolivia was made possible by the participation of different institutions like the Universidad Mayor de San Andrés, the Strategic Research Program in Bolivia, the Bolivian Catholic University, the Bolivian Association for Biomedical Journal Editors and the Vice Ministry of Science and Technology the support of the Pan American Health Organization (PAHO) Representation Bolivia.To be part of the this project Journal of the Selva Andina Research Society SciELO-Bolivia, and meet the requirements in the criteria, policies and procedures for application, acceptance and permanence of scientific journals in the SciELO collection - Bolivia, achieving desired by the Chairman of the Board of the Supreme Council on Science and Biotechnology Society Andean jungle, leaves open the space for Undergraduate Students, Graduate, Professional, and Investigators National Public and Private Institutions around the world to submit their articles for publication, because we have recognized domestic and foreign professionals, for arbitration and guaranteeing therefore the quality of the publication.The Chairman of the Board the Superior Council on Research & Science and Biotechnology of the Selva Andina Research Society and the Director and Editor of the Journal of the Selva Andina Research Society with the Director General of the Peasant Academic Unit of Carmen Pampa in representation of the Peasant Academic Unit Carmen Pampa Foundation do extend this Invitation. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived Journal of the Selva Andina Research Society Publisher An entity responsible for making the resource available Selva Andina Research Society Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Science, Biochemistry Language A language of the resource ES https://www.socictopen.socict.org/files/original/6eaaf3d0cac756590816d07f6ce929b9.pdf de12b27986c006d3b6f94d35c7cfd6ad Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Avaliação das principais causas de mortalidade de bezerras da raça Hostein em propriedades rurais da região bragantina Creator An entity primarily responsible for making the resource Daniela Moraes Oliveira Description An account of the resource O estudo foi conduzido em duas propriedades de exploração leiteira, ambas criadoras de bovinos da Raça Holstein pura origem (PO), localizadas no Município de Bragança Paulista – SP. A Fazenda 1 possui um plantel de 273 animais e a Fazenda 2 possui 450 animais. O estudo foi realizado em 11 animais (bezerras) de um a três meses de idade. Estes animais foram submetidos à necropsia, e amostras de tecido dos órgãos afetados foram coletados para realização de exames histopatológicos e cultivo microbiológico, além de coleta de sangue para exames sorológicos. O objetivo do estudo foi à identificação das principais causas de morte em bezerras do gado leiteiro da região Bragantina, onde foi possível avaliar e identificar as possíveis causas de óbitos. Dentre essas causas de mortes, as enterites apresentaram clinicamente uma freqüência similar, as doenças respiratórias. Conclui-se que ao ser avaliado as amostras anatomopatológicas identificaram-se uma freqüência maior de lesões macroscópicas e microscópicas pulmonares do que de lesões intestinais. Os agentes etiológicos associados com as doenças apresentadas foram a Salmonella a Escherichia coli, Clostridium perfringens, Pasteurella, vírus sincicial e coronavirus, que acometem o sistema respiratório e o sistema gastrointestinal, podendo levar o animal a desenvolver o quadro de septicemia e óbito. Date A point or period of time associated with an event in the lifecycle of the resource 2012 Subject The topic of the resource bezerras, Enterites, Necrópsia Identifier An unambiguous reference to the resource within a given context DOI: 10.5007/2175-7925.2012v25n2p171 Source A related resource from which the described resource is derived Biotemas Publisher An entity responsible for making the resource available Universidade Federal de Santa Catarina Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Science Language A language of the resource EN, ES, PT https://www.socictopen.socict.org/files/original/bf5bdd52a46c0564bd969e7e96ecc15a.pdf d175e02ed8dc6fde7a860dbf5053d5ba Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Persistence of lung inflammation and lung cytokines with high-resolution CT abnormalities during recovery from SARS Creator An entity primarily responsible for making the resource Lin Shu-Min, Lin Horng-Chyuan, Huang Kuo-Hsiung, Chou Chun-Liang, Wan Yung-Liang, Liu Chien-Ying, Wang Chun-Hua, Lin Tzou-Yien, Chung Kian, Kuo Han-Pin Description An account of the resource Abstract Background During the acute phase of severe acute respiratory syndrome (SARS), mononuclear cells infiltration, alveolar cell desquamation and hyaline membrane formation have been described, together with dysregulation of plasma cytokine levels. Persistent high-resolution computed tomography (HRCT) abnormalities occur in SARS patients up to 40 days after recovery. Methods To determine further the time course of recovery of lung inflammation, we investigated the HRCT and inflammatory profiles, and coronavirus persistence in bronchoalveolar lavage fluid (BALF) of 12 patients at recovery at 60 and 90 days. Results At 60 days, compared to normal controls, SARS patients had increased cellularity of BALF with increased alveolar macrophages (AM) and CD8 cells. HRCT scores were increased and correlated with T-cell numbers and their subpopulations, and inversely with CD4/CD8 ratio. TNF-α, IL-6, IL-8, RANTES and MCP-1 levels were increased. Viral particles in AM were detected by electron microscopy in 7 of 12 SARS patients with high HRCT score. On day 90, HRCT scores improved significantly in 10 of 12 patients, with normalization of BALF cell counts in 6 of 12 patients with repeat bronchoscopy. Pulse steroid therapy and prolonged fever were two independent factors associated with delayed resolution of pneumonitis, in this non-randomized, retrospective analysis. Conclusion Resolution of pneumonitis is delayed in some patients during SARS recovery and may be associated with delayed clearance of coronavirus, Complete resolution may occur by 90 days or later. Date A point or period of time associated with an event in the lifecycle of the resource 2005 Subject The topic of the resource SARS, alveolar macrophages, T lymphocyte, coronavirus, cytokines, bronchoalveolar lavage Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1465-9921-6-42 Source A related resource from which the described resource is derived Respiratory Research Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Diseases of the respiratory system Language A language of the resource EN https://www.socictopen.socict.org/files/original/dc12a3f206ea17d809aaf0a7673a1557.pdf ee2736a96f5806329e3fafe781166323 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource KrakenUniq: confident and fast metagenomics classification using unique k-mer counts Creator An entity primarily responsible for making the resource F. P. Breitwieser, D. N. Baker, S. L. Salzberg Description An account of the resource Abstract False-positive identifications are a significant problem in metagenomics classification. We present KrakenUniq, a novel metagenomics classifier that combines the fast k-mer-based classification of Kraken with an efficient algorithm for assessing the coverage of unique k-mers found in each species in a dataset. On various test datasets, KrakenUniq gives better recall and precision than other methods and effectively classifies and distinguishes pathogens with low abundance from false positives in infectious disease samples. By using the probabilistic cardinality estimator HyperLogLog, KrakenUniq runs as fast as Kraken and requires little additional memory. KrakenUniq is freely available at https://github.com/fbreitwieser/krakenuniq. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource metagenomics, Microbiome, Metagenomics classification, Pathogen Detection, Infectious disease diagnosis Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s13059-018-1568-0 Source A related resource from which the described resource is derived Genome Biology Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Genetics Language A language of the resource EN https://www.socictopen.socict.org/files/original/87d8057a388918a10fbcedf2da65e998.pdf 7045a22124c791f0142cf1ec763b2ae4 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Cambio de la línea de costa en el Bajo Segura (Sur de Alicante) en los últimos 15.000 años Creator An entity primarily responsible for making the resource Tent-Manclús, José Enrique Description An account of the resource This paper describes the change of the coastline in the southern part of the province of Alicante for the last 15,000 years. It is obtained by integrating data from different sources and especially high-resolution seismic profiles of the nearby marine continental shelf. Ten periods have been distinguished ranging from the marine transgression after the last glaciation, following the devellopment of sinus ilicitanus between 4,000 and 3,000 BC, and finally it begins a slow drying process until today, when it is reduced to the lagoons of the Fondo and Salinas of Santa Pola.En este trabajo se describe el cambio de la línea de costa en la parte sur de la provincia de Alicante para los últimos 15.000 años. Se ha obtenido integrando datos de diferentes fuentes y especialmente de perfiles sísmicos de alta resolución marinos de la plataforma continental anexa. Se han distinguido 10 periodos. Comienza con la transgresión marina tras la última glaciación, le sigue la formación del sinus ilicitanus entre los años 4000 y 3000 a. C., y finaliza con un lento proceso de desecación hasta la actualidad, reducido a las lagunas del Fondo y Salinas de Santa Pola.[fr] Dans ce travail on décrit l’évolution du littoral dans la partie sud de la province d’Alicante pour les 15.000 dernières années. Il a été obtenu en intégrant les données provennat de différentes sources et en particulier de l’étude des profils sismiques à haute résolution du plateau continental. Dix périodes ont été définis depuis le début de la transgression marine après la dernière glaciation. Le maximun transgressif abouti à la formation du sinus ilicitanus entre 4000 et 3000 avant JC. Depuis ce moment, commença un processus de dessiccation lent, qui se poursuit jusqu’à aujourd’hui, et qui a réduit l’ancien sinus a les actuelles lacunes du Fondo et les Salinas du Santa Pola. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource Bajo Segura, Alicante coast, Sea-level Change, coastal maps, Holocene, costa de Alicante, cambio del nivel del mar, mapas costeros, Holoceno, Alicante Côte, le changement du niveau des mers, Cartes côtières Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived Estudios Geográficos Publisher An entity responsible for making the resource available Consejo Superior de Investigaciones Científicas Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Geography (General) Language A language of the resource EN, ES https://www.socictopen.socict.org/files/original/e0104f3ad4c31754c262b66c2e4b04f2.pdf fa2c2590c7dfddcc6aaa4744ef2d43cd Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus Creator An entity primarily responsible for making the resource Zhang Zhibang, Chen Jianfei, Shi-hong Yan, Chen Xiaojin, Shi Da, Feng Li, Yang Bin Description An account of the resource Abstract Background The major structural protein of coronaviruses, the membrane (M) protein, can elicit the formation of protective antibodies, but little information is available about the M protein of porcine epidemic diarrhea virus (PEDV). Identification of epitopes on the PEDV M protein will be helpful in the elucidation of the antigenic properties of this protein. Results One hybridoma cell line secreting anti-M protein monoclonal antibody (McAb) was generated and designated 4D4. To map the epitopes on the PEDV M protein, a total of 17 partially overlapping fragments covering the C-terminus of M protein were expressed as fusion proteins with a 6×His tag or a GST tag. A linear motif, 193TGWAFYVR200, was identified by enzyme-linked immunosorbent assay (ELISA) and western blot (WB) analysis using McAb 4D4. The motif 195WAFYVR200 was the minimal requirement for reactivity, as demonstrated by removing amino acids individually from both ends of the motif 193TGWAFYVR200. The result of WB analysis showed that the 4D4-defined epitope could be recognized by PEDV-positive serum, but not transmissible gastroenteritis virus (TGEV)-positive serum. Furthermore, this epitope was highly conserved among different PEDV strains, as shown by alignment and comparison of sequences. Conclusion A McAb, 4D4, directed against the M protein of PEDV, was obtained, and the 4D4-defined minimal epitope sequence was 195WAFYVR200. The McAb could serve as a candidate for development of a McAb-based antigen capture ELISA for detection of PEDV. The epitope identified provides a basis for the development of epitope-based differential diagnostic techniques and may be useful in the design of epitope-based vaccines. Date A point or period of time associated with an event in the lifecycle of the resource 2012 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1743-422X-9-225 Source A related resource from which the described resource is derived Virology Journal Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN https://www.socictopen.socict.org/files/original/4b5f57d82f3f378e1d5c952a3e66c9be.pdf e0d7d33f0d363a7bdd52617734e0f57e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Measuring inequalities in the public health workforce at county-level Centers for Disease Control and Prevention in China Creator An entity primarily responsible for making the resource Weiqin Cai, Chengyue Li, Mei Sun, Mo Hao Description An account of the resource Abstract Background The public health workforce (PHW) is a key component of a country’s public health system. Since the outbreak of SARS (severe acute respiratory syndrome) in 2003, the scale of PHW in China has been continuously expanding, but policymakers and researchers still focus on the distribution of public health personnel, especially the regional inequality in such distribution. We aimed to identify the root cause of PHW inequality by decomposing different geographical units in China. Methods This study was based on data from a nationwide survey, which included 2712 county-level data. The distribution of the PHW in geographical units was evaluated by the Gini coefficient and Theil T index, and inequalities at regional, provincial, and municipal levels were decomposed to identify the root causes of inequalities in the PHW. Additionally, the contextual factors affecting the distribution of the PHW were determined through regression analysis. Results The overall inequality results show that health professional and field epidemiological investigators faced worse inequality than the staff. In particular, field epidemiological investigators had a Gini coefficient close to 0.4. Step decomposition showed that within-region inequalities accounted for 98.5% or more of overall inter-county inequality in the distribution of all PHW categories; provincial decomposition showed that at least 74% of inequality is still distributed within provinces; the overall contribution of within-municipal inequality and between-municipal inequality was basically the same. Further, the contextual factor that influenced between-municipality and within-municipality inequality for all three categories of PHWs was the agency building area per employee. Per capita GDP had a similar effect, except for between-municipality inequality of professionals and within-municipality inequality of field epidemiological investigators. Conclusions The successive decomposition showed that inequality is mainly concentrated in counties at the within-province and within-municipal levels. This study clearly suggests that the government, especially the municipal government at the provincial level, should increase financial investment in Centers for Disease Control and Prevention (CDCs) with worse resource allocation in their jurisdiction through various ways of compensation and incentives, enhance their infrastructure, and improve the salary of personnel in these institutions, to attract more public health professionals to these institutions. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource public health workforce, Inequalities decomposition, cdc, county level Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12939-019-1073-4 Source A related resource from which the described resource is derived International Journal for Equity in Health Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Public aspects of medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/48f02fd187b1ba50a00246050400de7a.pdf 73722c81fb4605197b37378dd1548799 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A dictionary based informational genome analysis Creator An entity primarily responsible for making the resource Castellini Alberto, Franco Giuditta, Manca Vincenzo Description An account of the resource Abstract Background In the post-genomic era several methods of computational genomics are emerging to understand how the whole information is structured within genomes. Literature of last five years accounts for several alignment-free methods, arisen as alternative metrics for dissimilarity of biological sequences. Among the others, recent approaches are based on empirical frequencies of DNA k-mers in whole genomes. Results Any set of words (factors) occurring in a genome provides a genomic dictionary. About sixty genomes were analyzed by means of informational indexes based on genomic dictionaries, where a systemic view replaces a local sequence analysis. A software prototype applying a methodology here outlined carried out some computations on genomic data. We computed informational indexes, built the genomic dictionaries with different sizes, along with frequency distributions. The software performed three main tasks: computation of informational indexes, storage of these in a database, index analysis and visualization. The validation was done by investigating genomes of various organisms. A systematic analysis of genomic repeats of several lengths, which is of vivid interest in biology (for example to compute excessively represented functional sequences, such as promoters), was discussed, and suggested a method to define synthetic genetic networks. Conclusions We introduced a methodology based on dictionaries, and an efficient motif-finding software application for comparative genomics. This approach could be extended along many investigation lines, namely exported in other contexts of computational genomics, as a basis for discrimination of genomic pathologies. Date A point or period of time associated with an event in the lifecycle of the resource 2012 Subject The topic of the resource Comparative genomics, Computational genomics, Genome clustering, information theory, Sequence analysis Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1471-2164-13-485 Source A related resource from which the described resource is derived BMC Genomics Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Genetics, Biotechnology Language A language of the resource EN https://www.socictopen.socict.org/files/original/ac8346d368526813fc8d5bbd9e7725f2.pdf 1d36c7f80365f5ed3a394d3bb6a050d9 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Cyclooxygenase activity is important for efficient replication of mouse hepatitis virus at an early stage of infection Creator An entity primarily responsible for making the resource Raaben Matthijs, Einerhand Alexandra WC, Taminiau Lucas JA, van Houdt Michel, Bouma Janneke, Raatgeep Rolien H, Büller Hans A, de Haan Cornelis AM, Rossen John WA Description An account of the resource Abstract Cyclooxygenases (COXs) play a significant role in many different viral infections with respect to replication and pathogenesis. Here we investigated the role of COXs in the mouse hepatitis coronavirus (MHV) infection cycle. Blocking COX activity by different inhibitors or by RNA interference affected MHV infection in different cells. The COX inhibitors reduced MHV infection at a post-binding step, but early in the replication cycle. Both viral RNA and viral protein synthesis were affected with subsequent loss of progeny virus production. Thus, COX activity appears to be required for efficient MHV replication, providing a potential target for anti-coronaviral therapy. Date A point or period of time associated with an event in the lifecycle of the resource 2007 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1743-422X-4-55 Source A related resource from which the described resource is derived Virology Journal Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN