212 10 20655 https://www.socictopen.socict.org/files/original/a0e6fc40d40480033f28418dba8340b8.pdf 8f458c5de07f858f985f674f8531be75 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway Creator An entity primarily responsible for making the resource Xiaona Wei, Gaoli She, Tingting Wu, Chunyi Xue, Yongchang Cao Description An account of the resource Abstract With the emergence of highly pathogenic variant strains, porcine epidemic diarrhea virus (PEDV) has led to significant economic loss in the global swine industry. Many studies have described how coronaviruses enter cells, but information on PEDV invasion strategies remains insufficient. Given that the differences in gene sequences and pathogenicity between classical and mutant strains of PEDV may lead to diverse invasion mechanisms, this study focused on the cellular entry pathways and cellular transport of the PEDV GI and GII subtype strains in Vero cells and IPEC-J2 cells. We first characterized the kinetics of PEDV entry into cells and found that the highest invasion rate of PEDV was approximately 33% in the IPEC-J2 cells and approximately 100% in the Vero cells. To clarify the specific endocytic pathways, systematic research methods were used and showed that PEDV enters cells via the clathrin- and caveolae-mediated endocytosis pathways, in which dynamin II, clathrin heavy chain, Eps15, cholesterol, and caveolin-1 were indispensably involved. In addition, lipid raft extraction assay showed that PEDV can also enter cells through lipid raft-mediated endocytosis. To investigate the trafficking of internalized PEDV, we found that PEDV entry into cells relied on low pH and internalized virions reached lysosomes through the early endosome–late endosome–lysosome pathway. The results concretely revealed the entry mechanisms of PEDV and provided an insightful theoretical basis for the further understanding of PEDV pathogenesis and guidance for new targets of antiviral drugs. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s13567-020-0739-7 Source A related resource from which the described resource is derived Veterinary Research Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Veterinary medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/62003f03f15e7407b92c20b0e7ff75a7.pdf 2fcd7e919d8018f2f2709e9e5e725927 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Dilemas éticos gerados pela SARS Creator An entity primarily responsible for making the resource Regina Abdulkader Date A point or period of time associated with an event in the lifecycle of the resource 2004 Identifier An unambiguous reference to the resource within a given context DOI: 10.1590/S0104-42302004000200002 Source A related resource from which the described resource is derived Revista da Associação Médica Brasileira Publisher An entity responsible for making the resource available Associação Médica Brasileira Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine (General) Language A language of the resource EN, ES, PT https://www.socictopen.socict.org/files/original/d311f9dc283bab33231cb25569f8dd37.pdf f254e2838af393e5ea92c0af463439d2 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource K-mer clustering algorithm using a MapReduce framework: application to the parallelization of the Inchworm module of Trinity Creator An entity primarily responsible for making the resource Chang-Sik Kim, Martyn D. Winn, Vipin Sachdeva, Kirk E. Jordan Description An account of the resource Abstract Background De novo transcriptome assembly is an important technique for understanding gene expression in non-model organisms. Many de novo assemblers using the de Bruijn graph of a set of the RNA sequences rely on in-memory representation of this graph. However, current methods analyse the complete set of read-derived k-mer sequence at once, resulting in the need for computer hardware with large shared memory. Results We introduce a novel approach that clusters k-mers as the first step. The clusters correspond to small sets of gene products, which can be processed quickly to give candidate transcripts. We implement the clustering step using the MapReduce approach for parallelising the analysis of large datasets, which enables the use of compute clusters. The computational task is distributed across the compute system using the industry-standard MPI protocol, and no specialised hardware is required. Using this approach, we have re-implemented the Inchworm module from the widely used Trinity pipeline, and tested the method in the context of the full Trinity pipeline. Validation tests on a range of real datasets show large reductions in the runtime and per-node memory requirements, when making use of a compute cluster. Conclusions Our study shows that MapReduce-based clustering has great potential for distributing challenging sequencing problems, without loss of accuracy. Although we have focussed on the Trinity package, we propose that such clustering is a useful initial step for other assembly pipelines. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Subject The topic of the resource MapReduce, De novo sequence assembly, RNA-Seq, Trinity Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12859-017-1881-8 Source A related resource from which the described resource is derived BMC Bioinformatics Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Computer applications to medicine. Medical informatics Language A language of the resource EN https://www.socictopen.socict.org/files/original/8f513a3f70485eb57c2021aca8881fc5.pdf 19982757b7e6a9946c889cacf454d450 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Middle East Respiratory Syndrome Coronavirus Transmission Creator An entity primarily responsible for making the resource Marie E. Killerby, Holly M Biggs, Claire M. Midgley, Susan I. Gerber, John T. Watson Description An account of the resource Middle East respiratory syndrome coronavirus (MERS-CoV) infection causes a spectrum of respiratory illness, from asymptomatic to mild to fatal. MERS-CoV is transmitted sporadically from dromedary camels to humans and occasionally through human-to-human contact. Current epidemiologic evidence supports a major role in transmission for direct contact with live camels or humans with symptomatic MERS, but little evidence suggests the possibility of transmission from camel products or asymptomatic MERS cases. Because a proportion of case-patients do not report direct contact with camels or with persons who have symptomatic MERS, further research is needed to conclusively determine additional mechanisms of transmission, to inform public health practice, and to refine current precautionary recommendations. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource MERS-CoV, Middle East respiratory syndrome, coronavirus, emerging infectious disease, healthcare-associated transmission, Dromedary Identifier An unambiguous reference to the resource within a given context DOI: 10.3201/eid2602.190697 Source A related resource from which the described resource is derived Emerging Infectious Diseases Publisher An entity responsible for making the resource available Centers for Disease Control and Prevention Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases, Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/13ff118fb91c0e616fcd725a944eeb3b.pdf 89153c184e8e039c24f1d6e11cc7c66f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource THREE NOTICEABLE ISSUES ON NOVEL CORONAVIRUS: A QUICK LOOK FROM VIETNAM’S CIRCUMSTANCES Creator An entity primarily responsible for making the resource Le Thi Thanh Tuyen Description An account of the resource N/A Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource coronavirus, Vietnam, Issue, nurse Identifier An unambiguous reference to the resource within a given context DOI: https://doi.org/10.33546/bnj.1058 Source A related resource from which the described resource is derived Belitung Nursing Journal Publisher An entity responsible for making the resource available Belitung Raya Foundation Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Nursing Language A language of the resource EN https://www.socictopen.socict.org/files/original/46189668e70b969831416cd2af0c4e5a.pdf 99449b3d8fb00b48f4403da37e8c1b51 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Site-Specific Cleavage of RNAs Derived from the <i>PIM1</i> 3′-UTR by a Metal-Free Artificial Ribonuclease Creator An entity primarily responsible for making the resource Felix Zellmann, Laura Thomas, Ute Scheffer, Roland K. Hartmann, Michael W. Göbel Description An account of the resource Oligonucleotide conjugates of tris(2-aminobenzimidazole) have been reported previously to cleave complementary RNA strands with high levels of sequence and site specificity. The RNA substrates used in these studies were oligonucleotides not longer than 29-mers. Here we show that ~150&#8315;400-mer model transcripts derived from the 3&#8242;-untranslated region of the PIM1 mRNA reacted with rates and specificities comparable to those of short oligonucleotide substrates. The replacement of DNA by DNA/LNA mixmers further increased the cleavage rate. Tris(2-aminobenzimidazoles) were designed to interact with phosphates and phosphate esters. A cell, however, contains large amounts of phosphorylated species that may cause competitive inhibition of RNA cleavage. It is thus important to note that no loss in reaction rates was observed in phosphate buffer. This opens the way to in-cell applications for this type of artificial nuclease. Furthermore, we disclose a new synthetic method giving access to tris(2-aminobenzimidazoles) in multigram amounts. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource 2-aminobenzimidazole, cleavage of large RNA molecules, cleavage site selection, DNA/LNA mixmers, dye labeling, guanidine analogs, oligonucleotides, specificity of cleavage Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/molecules24040807 Source A related resource from which the described resource is derived Molecules Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Organic chemistry Language A language of the resource EN https://www.socictopen.socict.org/files/original/2e4e97e9e364c55cb3061c4ea536f6e4.pdf 06aff2b250abb304fa8703567958ebb0 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Synthesis and In Vitro Antibacterial Activity of Quaternized 10-Methoxycanthin-6-one Derivatives Creator An entity primarily responsible for making the resource Na Li, Dan Liu, Jiang-Kun Dai, Jin-Yi Wang, Jun-Ru Wang Description An account of the resource Background: Based on our previous work, we found that 10-methoxycanthin-6-one displayed potential antibacterial activity and quaternization was an available method for increasing the antibacterial activity. Here, we explored the antibacterial activity of quaternized 10-methoxy canthin-6-one derivatives. Methods and Results: Twenty-two new 3-N-benzylated 10-methoxy canthin-6-ones were designed and synthesized through quaternization reaction. The in vitro antibacterial activity against three bacteria was evaluated by the double dilution method. Moreover, the structure&#8211;activity relationships (SARs) were carefully summarized in order to guide the development of antibacterial canthin-6-one agents. Two highly active compounds (6p and 6t) displayed 8-fold superiority (MIC = 3.91 &#181;g/mL) against agricultural pathogenic bacteria R. solanacearum and P. syringae compared to agrochemical streptomycin sulfate, and showed potential activity against B. cereus. Moreover, these two compounds exhibited good &#8220;drug-like&#8222; properties, low cytotoxicity, and no inhibition on seed germination. Conclusions: This work provides two new effective quaternized canthin-6-one derivatives as candidate bactericide, promoting the development of natural-sourced bactericides and preservatives. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource 10-methoxycanthin-6-one, quaternization, Antibacterial, SARS Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/molecules24081553 Source A related resource from which the described resource is derived Molecules Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Organic chemistry Language A language of the resource EN https://www.socictopen.socict.org/files/original/726ebbb10084222fcd7a68a435d5ba60.pdf 498a99883ed738b82f68ec52c823936b Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Potential Intermediate Hosts for Coronavirus Transmission: No Evidence of Clade 2c Coronaviruses in Domestic Livestock from Ghana Creator An entity primarily responsible for making the resource Philip El-Duah, Augustina Sylverken, Michael Owusu, Richmond Yeboah, Jones Lamptey, Yaw Oppong Frimpong, Vitus Burimuah, Christopher Antwi, Raphael Folitse, Olivia Agbenyega, Samuel Oppong, Yaw Adu-Sarkodie Description An account of the resource The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV), nearly a decade ago with worldwide distribution, was believed to be of zoonotic origin from bats with dromedary camels as intermediate hosts. There is a likelihood of other domestic livestock serving as intermediate hosts for this virus. The presence of coronaviruses, closely related to MERS-CoV in Ghanaian bats, presented the opportunity to test the hypothesis of transmissibility of this virus through domestic livestock species. The possible interactions between livestock and bats in 31 household farms were accessed by observation and interviews with farmers. Rectal swabs and serum from cattle, sheep, goats, donkeys, and swine from commercial and household farms were tested for MERS-CoV and a Nycteris sp. bat coronavirus, previously detected in Ghana. A pan-PCR assay to detect clade 2c viruses and recombinant immunofluorescence assay to detect anti-spike IgG antibodies against the target viruses were used. Likely contact between livestock and bats was determined for 13 farms (41.9%) that reported confining their livestock and also observing bats in their homes. Livestock were left unconfined on eight farms (25.8%) that also observed bats roosting in trees close to their homes. No viral RNA or antibodies against the two coronaviruses were detected in any of the livestock species tested. Cattle, sheep, goats, donkeys, and swine are not likely hosts of clade 2c coronaviruses. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource livestock, coronavirus, Intermediate host, bats Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/tropicalmed4010034 Source A related resource from which the described resource is derived Tropical Medicine and Infectious Disease Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine Language A language of the resource EN https://www.socictopen.socict.org/files/original/e460c3e9b25e3cab81d7e5d60a933f7d.pdf d3bd1ff4c206c81e46b3f9ca8f5081cc Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Enhanced Ability of Oligomeric Nanobodies Targeting MERS Coronavirus Receptor-Binding Domain Creator An entity primarily responsible for making the resource Lei He, Wanbo Tai, Jiangfan Li, Yuehong Chen, Yaning Gao, Junfeng Li, Shihui Sun, Yusen Zhou, Lanying Du, Guangyu Zhao Description An account of the resource Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV), an infectious coronavirus first reported in 2012, has a mortality rate greater than 35%. Therapeutic antibodies are key tools for preventing and treating MERS-CoV infection, but to date no such agents have been approved for treatment of this virus. Nanobodies (Nbs) are camelid heavy chain variable domains with properties distinct from those of conventional antibodies and antibody fragments. We generated two oligomeric Nbs by linking two or three monomeric Nbs (Mono-Nbs) targeting the MERS-CoV receptor-binding domain (RBD), and compared their RBD-binding affinity, RBD&#8315;receptor binding inhibition, stability, and neutralizing and cross-neutralizing activity against MERS-CoV. Relative to Mono-Nb, dimeric Nb (Di-Nb) and trimeric Nb (Tri-Nb) had significantly greater ability to bind MERS-CoV RBD proteins with or without mutations in the RBD, thereby potently blocking RBD&#8315;MERS-CoV receptor binding. The engineered oligomeric Nbs were very stable under extreme conditions, including low or high pH, protease (pepsin), chaotropic denaturant (urea), and high temperature. Importantly, Di-Nb and Tri-Nb exerted significantly elevated broad-spectrum neutralizing activity against at least 19 human and camel MERS-CoV strains isolated in different countries and years. Overall, the engineered Nbs could be developed into effective therapeutic agents for prevention and treatment of MERS-CoV infection. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource coronavirus, MERS-CoV, receptor-binding domain, therapeutic antibodies, nanobodies, cross-neutralization Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v11020166 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN https://www.socictopen.socict.org/files/original/1c7797510e8794db236c28f908147f36.pdf 71da82d0de6b87535e4bfcd365ea94ef Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Relevant factors for the suspension of the payment of disputed tax - A comparative analysis Creator An entity primarily responsible for making the resource Linda van Zyl, Danielle van Wyk Description An account of the resource Section 164(3) of the Tax Administration Act No. 28 of 2011 gives a senior SARS official the discretion to suspend the payment of disputed tax or a portion thereof, having regard to relevant factors. Limited guidance is available in this regard. The objectives are to establish the concerns and uncertainties regarding the relevant factors and to determine whether a ‘suspension of the payment of disputed tax’ in terms of section 164(3) constitutes the granting of ‘credit’ in terms of a ‘credit agreement’ in terms of the National Credit Act, Act No. 34 of 2005. This is achieved by adopting an explanatory research approach and performing a literature review and comparative analysis respectively. The conclusion is reached that the current factors listed are not necessarily the most relevant ones. Recommendations are made to simplify the process and to revise and improve the wording regarding the relevant factors. Date A point or period of time associated with an event in the lifecycle of the resource 2016 Subject The topic of the resource Section 164(3) of the Tax Administration Act No. 28 of 2011, National Credit Act, Act No. 34 of 2005, Request, suspend, Relevant Factors, defer, payment, credit Identifier An unambiguous reference to the resource within a given context DOI: 10.4102/jef.v9i2.59 Source A related resource from which the described resource is derived Journal of Economic and Financial Sciences Publisher An entity responsible for making the resource available African Online Scientific Information Systems Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Economics as a science Language A language of the resource EN