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                  <text>Dominio científico: Coronavirus</text>
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                <text>Virus-like particles of SARS-like coronavirus formed by membrane proteins from different origins demonstrate stimulating activity in human dendritic cells.</text>
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                <text>Bingke Bai, Qinxue Hu, Hui Hu, Peng Zhou, Zhengli Shi, Jin Meng, Baojing Lu, Yi Huang, Panyong Mao, Hanzhong Wang</text>
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                <text>The pathogenesis of SARS coronavirus (CoV) remains poorly understood. In the current study, two recombinant baculovirus were generated to express the spike (S) protein of SARS-like coronavirus (SL-CoV) isolated from bats (vAcBS) and the envelope (E) and membrane (M) proteins of SARS-CoV, respectively. Co-infection of insect cells with these two recombinant baculoviruses led to self-assembly of virus-like particles (BVLPs) as demonstrated by electron microscopy. Incorporation of S protein of vAcBS (BS) into VLPs was confirmed by western blot and immunogold labeling. Such BVLPs up-regulated the level of CD40, CD80, CD86, CD83, and enhanced the secretion of IL-6, IL-10 and TNF-alpha in immature dendritic cells (DCs). Immune responses were compared in immature DCs inoculated with BVLPs or with VLPs formed by S, E and M proteins of human SARS-CoV. BVLPs showed a stronger ability to stimulate DCs in terms of cytokine induction as evidenced by 2 to 6 fold higher production of IL-6 and TNF-alpha. Further study indicated that IFN-gamma+ and IL-4+ populations in CD4+ T cells increased upon co-cultivation with DCs pre-exposed with BVLPs or SARS-CoV VLPs. The observed difference in DC-stimulating activity between BVLPs and SARS CoV VLPs was very likely due to the S protein. In agreement, SL-CoV S DNA vaccine evoked a more vigorous antibody response and a stronger T cell response than SARS-CoV S DNA in mice. Our data have demonstrated for the first time that SL-CoV VLPs formed by membrane proteins of different origins, one from SL-CoV isolated from bats (BS) and the other two from human SARS-CoV (E and M), activated immature DCs and enhanced the expression of co-stimulatory molecules and the secretion of cytokines. Finding in this study may provide important information for vaccine development as well as for understanding the pathogenesis of SARS-like CoV.</text>
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                <text>10.1371/journal.pone.0002685</text>
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                <text>Korean Society of Epidemiology</text>
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                <text>Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication</text>
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                <text>Bingpeng Yan, Hin Chu, Dong Yang, Kong-Hung Sze, Pok-Man Lai, Shuofeng Yuan, Huiping Shuai, Yixin Wang, Richard Yi Tsun KAO, Jasper Fuk-Woo Chan, Kwok-yung Yuen</text>
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                <text>Lipids play numerous indispensable cellular functions and are involved in multiple steps in the replication cycle of viruses. Infections by human-pathogenic coronaviruses result in diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifestations. Understanding how cellular lipids may modulate the pathogenicity of human-pathogenic coronaviruses remains poor. To this end, we utilized the human coronavirus 229E (HCoV-229E) as a model coronavirus to comprehensively characterize the host cell lipid response upon coronavirus infection with an ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS)-based lipidomics approach. Our results revealed that glycerophospholipids and fatty acids (FAs) were significantly elevated in the HCoV-229E-infected cells and the linoleic acid (LA) to arachidonic acid (AA) metabolism axis was markedly perturbed upon HCoV-229E infection. Interestingly, exogenous supplement of LA or AA in HCoV-229E-infected cells significantly suppressed HCoV-229E virus replication. Importantly, the inhibitory effect of LA and AA on virus replication was also conserved for the highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV). Taken together, our study demonstrated that host lipid metabolic remodeling was significantly associated with human-pathogenic coronavirus propagation. Our data further suggested that lipid metabolism regulation would be a common and druggable target for coronavirus infections.</text>
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                <text>lipidomics, UHPLC–MS, HCoV-229E, MERS-CoV</text>
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                <text>DOI: 10.3390/v11010073</text>
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                <text>MDPI AG</text>
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                <text>Reply to “Better Safe than Sorry: Interventional Radiology Should Be Prepared for the Coronavirus Disease 2019 Pandemic”</text>
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                <text>Binit Sureka, Sarbesh Tiwari, Pawan K Garg</text>
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                <text>DOI: 10.1055/s-0040-1713696</text>
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                <text>Journal of Clinical Interventional Radiology ISVIR</text>
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                <text>Thieme Publishers</text>
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                <text>Medical physics. Medical radiology. Nuclear medicine</text>
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                <text>Why tocilizumab could be an effective treatment for severe COVID-19?</text>
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                <text>Binqing Fu, Xiaoling Xu, Haiming Wei</text>
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                <text>Abstract A severe pneumonia-associated respiratory syndrome caused by a new coronavirus was identified in December 2019 (COVID-19), spread rapidly and has become a world-wide public health challenge. About 25% of COVID-19 patients experienced severe complications including acute respiratory distress syndrome (ARDS), and even progressed into an intensive care unit (ICU) admission and died. The exploration for the mortality causes and advancing novel therapeutic development of severe COVID-19 is crucial at the moment. The biopsy samples analysis at autopsy suggested that increased alveolar exudate caused by aberrant host immune response and inflammatory cytokine storm probably impedes alveolar gas exchange and contributes to the high mortality of severe COVID-19 patients. Our research has identified that pathogenic T cells and inflammatory monocytes incite inflammatory storm with large amount of interleukin 6, therefore monoclonal antibody that targets the IL-6 pathways may potentially curb inflammatory storm. Moreover, Tocilizumab treatment that blocking IL-6 receptors showed inspiring clinical results including temperature returned to normal quickly and respiratory function improved. Therefore, we suggest that Tocilizumab is an effective treatment in severe patients of COVID-19 to calm the inflammatory storm and reduce mortality.</text>
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                <text>DOI: 10.1186/s12967-020-02339-3</text>
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                <text>Journal of Translational Medicine</text>
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                  <text>Agricultura sostenible</text>
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                <text>Nunca imaginé tener que escribir un obituario para un colega y amigo. Alejandro nació en Villavicencio hijo de Pablo Emilio Chaparro Sanchéz, un sargento segundo del ejercito, y de Nubia Giraldo, quien creció escondiendo revistas y libros en una caja debajo de la cama, forjando un hábito que impactaría en el pequeño: La lectura. Su padre, aficionado a la historia militar, había nombrado a la mayoría de sus hijos con el nombre de viejos guerreros que admiraba. Así nombró al pequeño Leonidas, a Bismarck, el menor, y a Harold, el único que lo sobrevivió. Y allí estaba Alejandro, el Grande, el fundador de Alejandría. Alejandro: Aleicos = protector, Antropos = de los hombres. Durante su infancia acompañó al abuelo y a su padre recogiendo papas con las manos desnudas en Pesca, Boyacá, portando la ruana y el sombrero. Ya en Bogotá estudió en el colegio Alamano, pasando su infancia entre el Galán y el Colón, donde estuvo en un grupo de música Andina. Se graduó de Ingeniero Agrónomo de la Universidad del Tolima, Magister en Ciencias Agrarias, línea fitomejoramiento en la Universidad Nacional de Colombia y Doctor en Genética y Mejoramiento Vegetal en la Universidad de Sao Paulo, y desde 1999 profesor del departamento de Biología de la Universidad Nacional de Colombia (UN). Para la fecha de su fallecimiento ostentaba la máxima categoría posible en la UN, la de profesor titular-Tenure; y la máxima en el sistema de calificación de investigadores en MinCiencias la de investigador Senior.   En su juventud por los años 80 se unió a la Juventud patriótica, siempre estuvo acompañando los consejos estudiantiles, e incluso liderando su representación, como en su doctorado, donde fungió como representante de estudiantes de posgrado extranjeros en el Campus de Piracicaba de la Universidad de Sao Paulo, posteriormente cuando ingresó a la Universidad Nacional de Colombia en diversas ocasiones fue representante de profesores del Departamento de Biología.   Estudioso de los derechos de propiedad intelectual. Ofertó con gran éxito los cursos de: Cultivos transgénicos: Frankenstein o Prometeo?, Política y Legislación en Recursos Naturales, Recursos Genéticos: elementos técnicos y jurídicos, Fundamentos genética y Genética.    Su carrera científica estuvo plagada de logros y primicias en su campo para Colombia y el mundo. Como la primera planta transgénica de maiz off patent. Sus temas de interés rondaban acerca de la libertad de uso de patentes, la propiedad intelectual, acceso a recursos genéticos, organismos genéticamente modificados y plantas transgénicas donde trabajó con variedades colombianas de maíz y papa principalmente. Creador y director del grupo de investigación de Ingeniería Genética de Plantas (IGP) donde formó por los menos 50 estudiantes entre pregrado, maestría y doctorado; fortaleciendo el conocimiento de la biotecnología vegetal en Colombia. Además también hacía parte de grupo de investigación Plebio, y era miembro del centro de pensamiento en propiedad intelectual de la mencionada Universidad.   Siempre se preocupó por la transferencia del conocimiento, y estaba convencido de la obligación de que como investigadores tenemos con la sociedad; es por lo que investigó los problemas que aquejaban a los agricultores, trabajó de la mano de gremios como la Federación Nacional de Cultivadores de Cereales, Leguminosas y Soya (FENALCE), Federación nacional de arroceros (FEDEARROZ), Centro internacional de la papa. Realmente es sorprendente la gran cantidad de trabajo que tenía adelantado en diversas áreas de su investigación, el número de estudiantes que dirigía y la gran interdisciplinariedad de sus investigaciones.   Aunque tenía el potencial para publicar ampliamente en revistas internacionales, prefirió las revistas nacionales. En Acta Biológica Colombiana publicó 19 manuscritos; siendo el artículo de revisión Cultivos transgénicos: entre los riesgos    biológicos y los beneficios ambientales y económicos, publicado en 2011 uno de los más citados, con 29 citas hasta el momento.   El lado menos académico del profesor, es recordado por su amplia y constante sonrisa, le gustaba “falar em portugués” cuando encontraba un colega que le llevara la cuerda, un bailador de salsa (incluso fue fundador de un frustrado bar de salsa), y apasionado del rock en español. Amante de las reuniones sociales, la cerveza, las discusiones de política y de los buenos libros. Padre de dos hijos que eran su orgullo (León Felipe y Jenny Katherine) y hacía poco menos de una década se había estrenado como abuelo (Niko Alejandro), que sacó de sus entrañas su lado mas tierno. Pasó los mejores años de su vida junto a su esposa “La pecosa”; Lucero López el amor de su vida.    Deja un gran vacío inmenso en la comunidad, pero un gran legado académico y de valores por seguir. Pero esté donde esté, debe tener la satisfacción de la labor cumplida. Descansa en paz, amigo.       Escrito por: Nubia Matta Camacho</text>
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                <text>Desastres, desplazamiento interno y migración laboral en la Sierra de Chiapas</text>
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                <text>Derivado de un escenario global de cambio climático, el huracán Stan (2005) impactó severa - mente a la región Sierra en el estado de Chiapas y devino en el mayor desplazamiento interno  de personas de la entidad. Una parte de la población de la región sufrió una repentina pérdida de  sus bienes y fue reubicada en lugares circunvecinos en medio de acciones improvisadas por parte  de organismos públicos, sin atender los protocolos internacionales en la materia. Para analizar el  proceso de reubicación y sus consecuencias, se llevó a cabo un estudio de desplazados en dos co - munidades denominadas “fraccionamientos”. En los nuevos lugares de asentamiento, los  medios  de vida  existentes fueron más precarios que los de los anteriores lugares de residencia, motivo  por el cual los desplazados no han podido acceder a satisfactores de bienestar general ni man - tener  el  acostumbrado  patrón  de  migración  internacional  que  antes  del  desplazamiento  tenían.   En cambio, las actuales condiciones han propiciado una migración tipo  commuter , situación que  desde décadas atrás, esa misma población había superado.</text>
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                <text>Papeles de Población</text>
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                <text>Universidad Autonoma del Estado de Mexico</text>
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                <text>Economic theory. Demography, Demography. Population. Vital events</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Sarsılmış Bebek Sendromu Süt Çocukluğu Döneminde Konvulsiyon Çocuk İstismarı İlişkisi Üç Adli Olgu Sunumu</text>
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                <text>Birgül   Tüzün, Raif Üçsel, Nevzat   Alkan, Agop Çitak, Şebnem Korur Fincancı</text>
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                <text>Süt çocukluğu dönemi fiziksel istismar olgularında, harici travmatik değişimlerin görülme olasılığı oldukça düşüktür. Olgular, daha çok aniden ortaya çıktığı söylenen ve hu yaş grubuna özgü çeşitli hastalık tablolarında gözlenen klinik bulgular sebebiyle özellikle çocuk acil-yoğun bakım ünitelerine getirilmektedir. Bu çalışmada, İstanbul Üniversitesi İstanbul Tıp Fakültesi Çocuk Acil-Yoğun Bakım Ünitesi'ne konvulsiyon klinik bulgusu ile başvuran süt çocukluğu dönemindeki 1 erkek, 2 kız çocuğu fiziksel istismar yönünden kliniğe yansıma şekli ve doğru tanıya yönelme aşamasında adli tıbbi yaklaşımın önemini vurgulamak ve belgelemek amacıyla sunulmuştur. Her üç olguda da, yakın zamanda düşme hikayesi, beyin tomografisinde subdural hematom ve göz dibi incelemesinde yaygın retinal kanama tespit edilmiştir. Öykü derinleştirildiğinde, olguların çocuk istismarı açısından izlenmesinin uygun olacağı sonucuna varılmıştır. Süt çocukluğu dönemi fiziksel istismar olgularında gecikmeden doğru tanı konulmasında, adli olgu olarak değerlendirilip, tespit edilen travmatik değişimlerin adli rapor şeklinde belgelendirilmesinde ve adli makamlara bildirilmesinde Adli Tıp Anabilim Dalları ile birlikte yürütülen koordine çalışmaların ivedilikle yaygınlaştırılması gerektiği düşünülmektedir.  Anahtar Kelimeler: Çocuk istismarı, subdural hematom, sarsılmış bebek sendromu.</text>
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                <text>2000</text>
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                <text>çocuk istismarı, subdural hematom, sarsılmış bebek sendromu</text>
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                <text>DOI: 10.17986/blm.200053423</text>
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                <text>Adli Tıp Bülteni</text>
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                <text>Adli Tıp Uzmanları Derneği</text>
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                <text>Medicine (General)</text>
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                <text>Valoración ecológica de los servicios ecosistémicos prestados por el suelo en fincas cafeteras en Belén de Umbría, Colombia</text>
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                <text>Bis Davidson Bedoya-Gómez, Miguel Angel Dossman-Gil, Jeferson Marín-Fernández</text>
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                <text>[Introducción]: Los beneficios que presta el suelo por medio de los servicios ecosistémicos de regulación, son importantes para el desarrollo de los sistemas productivos de café y son estratégicos para la adaptación al cambio climático. [Objetivo]: En la presente investigación se buscó valorar ecológicamente los servicios ecosistémicos prestados por el suelo en fincas cafeteras de La Cuchilla de San Juan. [Metodología]: Se colectó información en dos periodos de tiempo sobre las propiedades físicas, químicas y biológicas de los suelos de 15 predios. Se delimitaron 3 unidades de análisis denominadas ventanas, donde se determinaron 6 servicios ecosistémicos (disponibilidad de nutrientes, de agua, de actividad microbiológica, capacidad de enraizamiento, resistencia a la erosión y sumidero de dióxido de carbono). Se definieron las variables con mayor peso en la variabilidad de los servicios ecosistémicos y se realizó un proceso de estandarización para el manejo adecuado de la información. Se generaron tres índices (óptimo, de comportamiento y de gestión). [Resultados]: Se identificó que la disponibilidad de nutrientes y de actividad microbiológica es baja, la resistencia a la erosión y sumidero de dióxido de carbono varía entre bajo y medio, en tanto, la disponibilidad de agua y capacidad de enraizamiento varía entre medio y alto. El predio El Consuelo, obtuvo la mejor valoración; La Mirla, Los Pinos y El Progreso fueron los predios con menor valoración. [Conclusiones]: Se pudo evidenciar que las dinámicas antrópicas han incidido directamente en las condiciones del suelo, y por ello, es importante la planificación predial de los territorios.</text>
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                <text>ecosistema; índices; predio; servicios de regulación; sistema de producción</text>
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                <text>10.15359/rca.55-1.8</text>
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                <text>Universidad Nacional, Costa Rica</text>
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                  <text>Agricultura sostenible</text>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>PERCEPÇÃO AMBIENTAL DOS AGRICULTORES FAMILIARES E O USO DOS RECURSOS NATURAIS DO MUNICÍPIO DE SÃO DOMINGOS – SEMIÁRIDO BAIANO</text>
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                <text>Bismarque Lopes Pinto, Gabriel Matos Lima, Juliana Araújo Santos, Marcos Paulo Souza Novais</text>
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                <text>RESUMO A percepção sobre o meio no qual se produz torna-se fundamental para a compreensão acerca do desenvolvimento de tais práticas agrícolas, pois os usos dos recursos naturais tendem a seguir a lógica da percepção do objeto do qual se tem contato imediato. Objetivou-se nessa pesquisa compreender a percepção ambiental de agricultores familiares do município de São Domingos/BA e sua influência nas intervenções agrícolas por eles desenvolvidas, bem como a inserção de técnicas agroecológicas trazidas pelo Sindicato dos Trabalhadores Rurais da Agricultura Familiar de São Domingos (SINTRAF-SD) nas propriedades agrícolas onde há a presença da agricultura familiar. A pesquisa está pautada nos seguintes procedimentos metodológicos: I) Revisão de Literatura sobre os principais temas abordados no estudo; II) Coleta de dados sobre a organização estrutural dos projetos executados pelo SINTRAF-SD para os agricultores familiares; III) Entrevista Estruturada junto ao presidente do SINTRAF-SD; IV) Entrevista Estruturada com 10 representantes de 10 grupos familiares os quais se classificam enquanto agricultores familiares. A percepção ambiental dos agricultores familiares vem passando por uma mudança de paradigma consistente, tendo em vista que as antigas práticas exercidas por eles ou pelos seus antecedentes não são consideradas práticas sustentáveis sob o ponto de vista ambiental que, por sua vez, os levaram a uma gradativa mudança nos modos de perceber o sistema ambiental e como suas ações estavam degradando a natureza em geral.  Palavras-Chave: Agricultura Familiar; Percepção; Natureza.   PERCEPTION ENVIRONMENTAL FAMILY FARMERS AND THE USE OF NATURAL RESOURCES OF SÃO DOMINGOS CITY - SEMIARID BAIANO  ABSTRACT The perception of the environment in which it produces is fundamental to understanding the development of agricultural practices such as the use of natural resources tend to follow the logic of the perception of the object which has immediate contact. The objective was to this research to understand the environmental awareness of farmers in the municipality of São Domingos / BA and its influence on agricultural interventions they developed, as well as the inclusion of agroecological techniques brought by the Union of Rural Workers of Family Agriculture of São Domingos (SINTRAF -SD) on farms where there is the presence of family farming. The research is guided in the following methodological procedures: I) Review of Literature on the main issues addressed in the study; II) Data collection on the structural organization of the projects executed by SINTRAF-SD for family farmers; III) Structured Interview with President of SINTRAF-SD; IV) Structured Interview with 10 representatives from 10 family groups which are classified as family farmers. Environmental awareness of farmers has undergone a change of consistent paradigm, given that the old practices exercised by them or by their background sustainable practices are not considered from an environmental point of view that, in turn, led to a gradual change in the ways of perceiving the environmental system and how their actions were degrading nature in general.  Kewords: Family Farming; Perception; Nature.  PERCEPCIÓN AMBIENTAL DE LOS AGRICULTORES FAMILIARES Y EL USO DE LOS RECURSOS NATURALES DE LA CIUDAD DE SANTO DOMINGO - SEMIÁRIDO BAIANO  RESUMEN La percepción del medio ambiente en el que se produce es fundamental para comprender el desarrollo de las prácticas agrícolas tales como el uso de los recursos naturales tienden a seguir la lógica de la percepción del objeto que tiene contacto inmediato. El objetivo era esta investigación para comprender la conciencia medioambiental de los agricultores en el municipio de Santo Domingos / BA y su influencia en las intervenciones agrícolas que desarrollaron, así como la inclusión de técnicas agroecológicas interpuesto por la Unión de Trabajadores del Campo de la Agricultura Familiar de Santo Domingo (SINTRAF del SD) en la propriedad donde existe la presencia de la agricultura familiar. La investigación se guía en los siguientes procedimientos metodológicos: I) Revisión de bibliografía sobre los principales temas abordados en el estudio; colección II) Los datos sobre la organización estructural de los proyectos ejecutados por SINTRAF-SD para la agricultura familiar; III) entrevista estructurada con el presidente de SINTRAF-SD; IV) Entrevista estructurada con 10 representantes de grupos de 10 familias que se clasifican como agricultores familiares. La conciencia ambiental de los agricultores ha sufrido un cambio de paradigma coherente, dado que las viejas prácticas ejercidas por ellos o por sus prácticas sostenibles por su vez no se consideran desde el punto de vista del medio ambiente que, a su vez, dio lugar a una cambio gradual en las formas de percibir el sistema ambiental y de cómo sus acciones fueron degradando la naturaleza en general.  Palabras Clave: Agricultura Familiar; La Percepción; La Naturaleza.</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>Agricultura Familiar, Family farming, La Percepción, Perception, la naturaleza, nature, natureza, percepção</text>
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                <text>Universidade Federal do Maranhão</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Geography (General), Environmental sciences</text>
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                <text>&lt;a href="http://www.periodicoseletronicos.ufma.br/index.php/interespaco/article/view/5289" target="_blank" rel="noreferrer noopener"&gt;http://www.periodicoseletronicos.ufma.br/index.php/interespaco/article/view/5289&lt;/a&gt;</text>
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                <text>A sustainable mental health care delivery model for severe mental illness during COVID-19.</text>
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                <text>Biswa Ranjan Mishra, Tathagata Biswas, Rajeev Ranjan</text>
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                <text>10.1016/j.ajp.2021.102594</text>
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                <text>Asian journal of psychiatry</text>
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