https://www.socictopen.socict.org/files/original/2e5b3ae00d7d7e2262a5d442e398f99c.pdf 82038c95dd3bcaecd883a29189973d3e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Expresión de la proteína Cry1Ac en tejidos de líneas transgénicas de papa (Solanum tuberosum spp. Andígena) Var. Diacol capiro. Creator An entity primarily responsible for making the resource Alejandro Chaparro Giraldo, Pablo Andres Vanegas Araujo, Jennifer Teresa Blanco Martinez Description An account of the resource RESUMEN La papa (Solanum sp.) es el cuarto producto alimenticio más importante en el mundo. En Colombia anualmente se producen alrededor de 2,8 millones de toneladas, sirviendo como sustento económico a 90.000 familias. En el país, Tecia solanivora genera el mayor impacto económico en el cultivo con pérdidas de hasta el 100% en la producción de tubérculos. El fitomejoramiento vía introducción de genes Cry, que codifican para cristales proteicos insecticidas, constituye una alternativa para reducir el ataque de insectos en cultivos de interés comercial. En este trabajo se caracterizó la inserción, transcripción y expresión del gen Cry1Ac en diferentes tejidos y en tres etapas del desarrollo para dos líneas transgénicas de Solanum tuberosum spp. andígena variedad Diacol Capiro. La caracterización se realizó a través de técnicas de PCR, RT-PCR y ELISA. Se corroboró la inserción y transcripción del gen utilizando primers que amplificaron una banda específica de 766pb para Cry1Ac. Los niveles de expresión de la proteína llegaron a ser mayores a 45μg/g y no mostraron diferencias significativas entre las líneas analizadas, ni entre las tres etapas del desarrollo. No se evidenciaron diferencias significativas entre las líneas transgénicas con respecto al control al hacer un análisis de algunas características fenotípicas relevantes. Los resultados encontrados sugieren la realización de seguimientos y ensayos de bioseguridad sobre este material, ya que los altos niveles de expresión en todos los tejidos analizados, pueden afectar a organismos no blanco.   ABSTRACT Potato is the fourth of the most important crop and foodstuff in the world, in Colombia around 2.8 millions of tons are produced annually supporting economically 90000 families. In the country, the major economic impact in the crop is caused by Tecia solanivora that originates loses up to 100% in the tuber production. The genetic plant breeding related to the introduction of Cry genes which codify insecticidal crystal proteins is an alternative for reducing the insect attack in commercial crops. In this work, the insertion, transcription and expression of Cry1Ac gen was characterized in different tissues and three development stages of two transgenic lines of Solanum tuberosum varieties Diacol Capiro and Parda Pastusa that were transformed with that gen. The characterization was realized by PCR, Rt-PCR and ELISA techniques. The gen insertion and transcription was confirmed using primers for Cry1Ac gen that amplified a specific band of 766 bp. The protein expression levels were higher than 45 μg/g and were not significantly different between the analyzed lines neither the three development stages. Furthermore, taking in account some relevant phenotypic features, no significant differences were found between transgenic lines and controls. The results suggests monitoring and biosecurity assays with this vegetal material because their high level expression and inside all of tissues analyzes that could affect non-targeted insects.   Palabras clave: S. tuberosum, Diacol Capiro, Cry1Ac, caracterización molecular, cultivos transgénicos. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Subject The topic of the resource Cry1Ac, S. tuberosum, caracterización molecular, cultivos transgénicos, diacol-capiro Source A related resource from which the described resource is derived Acta Biológica Colombiana Publisher An entity responsible for making the resource available Universidad Nacional de Colombia Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General) Relation A related resource <a href="https://revistas.unal.edu.co/index.php/actabiol/article/view/10473" target="_blank" rel="noreferrer noopener">https://revistas.unal.edu.co/index.php/actabiol/article/view/10473</a>