Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells

Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells

Artur Szczepanski, Katarzyna Owczarek, Aleksandra Milewska, Zbigniew Baster, Zenon Rajfur, Judy A Mitchell, Krzysztof Pyrc

Abstract Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.

2018

DOI: 10.1186/s13567-018-0551-9

Veterinary Research

BMC

Veterinary medicine

EN