https://www.socictopen.socict.org/files/original/fca6ed92e81cc0aa13aa9bf6e4296ee1.pdf 684a629f95aae92391e587dc18735340 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A reverse transcription loop-mediated isothermal amplification (LAMP) assay for the detection of feline Coronavirus Creator An entity primarily responsible for making the resource Angelica Stranieri, Stefania Lauzi, Alessia Giordano, Saverio Paltrinieri Description An account of the resource Loop-mediated isothermal amplification (LAMP) is a molecular method that amplifies DNA under isothermal conditions. It relies on the use of 4 different primers recognizing 6 regions of the template sequence and on the use of a DNA polymerase with strand displacement activity (Notomi et al., 2000). The addition of two loop primers allows the reaction time to be of one hour only (Nagamine et al., 2002). The aim of this study was to develop a reverse transcription LAMP assay for an easy and inexpensive detection of feline Coronavirus (FCoV). Six primers binding the conserved 3’UTR region of the FCoV were designed with the Primer Explorer software. Thirty-two samples of RNA (11 feces, 8 effusions, 9 blood samples and 4 tissues) on which a reverse transcription polymerase chain reaction (RT-PCR) for the 3’UTR region was performed were used. The reaction was carried out in 25μL reaction volume and the mixture was incubated in a thermocycler at 63°C for 1 hour followed by 10 minutes at 80°C. LAMP products were visualized under UV after electrophoresis migration on a 1.5% agarose gel stained with ethidium bromide, where they produce a ladder-like pattern if positive. Results where compared with those obtained on standard PCR. Sensitivity and specificity were respectively 60% and 100% on feces, 40% and 100% on effusions, 25% and 100% on blood, and 100% and 100% on tissues. The overall sensitivity and specificity of this method were of 57.1% and 100%, thus limiting a clinical application of this method, except for tissues. Date A point or period of time associated with an event in the lifecycle of the resource 2016 Subject The topic of the resource LAMP, Feline coronavirus, PCR Identifier An unambiguous reference to the resource within a given context DOI: 10.13130/2283-3927/7075 Source A related resource from which the described resource is derived International Journal of Health, Animal Science and Food Safety Publisher An entity responsible for making the resource available Università degli Studi di Milano Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Genetics, Ecology, Animal culture, Economic biology Language A language of the resource EN