https://www.socictopen.socict.org/files/original/0dcbc3f7c6d0fb0d52cbf54968445e83.pdf e7358daed5fb29fde748c3e043b5f106 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Estudios de transformación genética en arveja voluble cultivar Santa Isabel Creator An entity primarily responsible for making the resource María Isabel Peñaranda, Gustavo Adolfo Ligarreto, Víctor Manuel Nuñez Description An account of the resource Título corto: Estudios de transformación genética en arveja.Resumen: Se probaron diferentes alternativas de transformación genética en arveja cultivar “Santa Isabel” con el fin de estudiar los factores que afectan el proceso. Se emplearon los métodos de infiltración mediante vacío, infección directa de explantes, transformación de polen, y microinyección de ovarios. La prueba histoquímica de expresión gus fue escogida como método de análisis en la determinación de transformantes positivos. Con las metodologías empleadas se detectaron puntos azules en el tejido vegetal, lo cual indica la expresión transitoria del transgen en los explantes utilizados. Los resultados obtenidos sugieren que la transformación genética en arveja cultivada en Colombia puede ser utilizada para la introducción de genes de interés como apoyo a los procesos de mejoramiento genético. Palabras clave: genotipos, expresión gus, fitomejoramiento. Abstract: Different genetic transformation alternatives were tested in “Santa Isabel” cultivar pea, in order to study the factors that affect the process. The methods used were infiltration with vacuum, direct infection of the explants, pollen transformation and ovary microinjection. The hystochemical test of  gus expression was chosen as analysis method in the determination of positive transformants. Blue spots in the plant tissue were detected, with the used methodologies, which indicates transient expression of the transgene in the explants utilized. The obtained results suggest that the genetic transformation in pea genotypes planted in Colombia can be used for the introduction of genes of interest as support to genetic improvement. Key words: genotypes, gus expressions, plant breeding. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource genotypes, gus expressions, plant breeding Identifier An unambiguous reference to the resource within a given context 10.15446/rev.colomb.biote.v15n2.41264 Source A related resource from which the described resource is derived Revista Colombiana de Biotecnología Publisher An entity responsible for making the resource available Universidad Nacional de Colombia Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biotechnology Relation A related resource <a href="https://revistas.unal.edu.co/index.php/biotecnologia/article/view/41264" target="_blank" rel="noreferrer noopener">https://revistas.unal.edu.co/index.php/biotecnologia/article/view/41264</a>