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                <text>Agricultura sostenible</text>
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                <text>Dominio científico: Agricultura sostenible</text>
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          <name>Title</name>
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              <text>Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk</text>
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          <name>Creator</name>
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              <text>Orlando Bernal Chia, Fernando  Murcia Rubiano, Olga Lucía  Ávila Guzmán, Jenifer Yaneth  Guzman Gualteros, Willinton Arturo  Arias Casallas, Maria Victoria Morales</text>
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              <text>BACKGROUND: The information on official testing methods, or regulatory methods in Colombia to test whey in milk is limited; this restriction of information goes against the possibility of mitigating the risk of food fraud. OBJECTIVES: The validation of an HPLC method to determine casein glycomacropeptide (c-GMP), a protein that countries such as Brazil, Spain, and Ecuador have used as an indicator of raw milk adulteration with whey, was carried out. METHODS: A 10mL sample of raw milk is precipitated with 24% TCA using ultrasound, a process followed by filtration. The collected fraction ensured the separation of c-GMP and then injected into the liquid chromatography. RESULTS: A 30 minutes analysis allowed the determination of c-GMP with a retention time of 12.9 ± 0.5 minutes. The performance characteristics method in the validation exercise were: recovery percentage 99.97%, linearity R2&gt; 0.95; % RSD accuracy</text>
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              <text>2021</text>
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              <text>Fraud food, High Pressure Liquid Cromatographic - HPLC, Whey milk, casein glycomacropeptide (CGMP), raw bovine milk</text>
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            <elementText elementTextId="227665">
              <text>10.17533/udea.vitae.v28n3a345256</text>
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          <name>Source</name>
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              <text>Vitae</text>
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          <name>Publisher</name>
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              <text>Universidad de Antioquia</text>
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          <name>Coverage</name>
          <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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            <elementText elementTextId="227668">
              <text>Food processing and manufacture, Pharmaceutical industry</text>
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          <description>A related resource</description>
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              <text>&lt;a href="https://revistas.udea.edu.co/index.php/vitae/article/view/345256" target="_blank" rel="noreferrer noopener"&gt;https://revistas.udea.edu.co/index.php/vitae/article/view/345256&lt;/a&gt;</text>
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