Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.

Jesse J. Waggoner, Malaya K. Sahoo, Benjamin A. Pinsky, Ahmed Babiker, Evan J. Anderson, Nadine Rouphael, Colleen S. Kraft, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Laila Hussaini

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.

2020

Respiratory Infections, Viruses, Zoonoses, coronavirus, molecular detection, SARS, rRT-PCR, SARS-CoV-2, COVID-19, Severe Acute Respiratory Syndrome Coronavirus 2, 2019 novel coronavirus disease, Coronavirus disease

DOI: 10.3201/eid2607.201285

Emerging Infectious Diseases

Centers for Disease Control and Prevention