Biological nitrogen fixation by Rhizobium sp. native gliricidia (Gliricidia sepium [Jacq.] Kunth ex Walp.) under greenhouse conditions

Biological nitrogen fixation by Rhizobium sp. native gliricidia (Gliricidia sepium [Jacq.] Kunth ex Walp.) under greenhouse conditions

Milian Mindiola Pablo Ernesto, Cubillos Hinojosa Juan Guillermo, Hernández Mulford Jorge Luis

<p class='MsoNoSpacing' style='text-align: justify; margin: 0cm 0cm 0pt;'><span style='mso-ansi-language: EN-US;' lang='EN-US'><span style='font-size: small;'><span style='font-family: Calibri;'>An experiment was conducted under greenhouse conditions in order to evaluate the association and biological nitrogen fixation (BNF), six native strains of <em>Rhizobium </em>sp isolated from nodules gliricidia and a commercial strain (Rhizobiolc). Suspensions of 106 and 108 cells/mL for each of the strains studied and applied in two separate trials: the first with seeds and the second with seedlings 35 days of age gliricidia. In the seed test is calculated the cumulative percentage of germination for 7 days. In both trials after 42 days in seeds and seedlings 52 days in length was measured and stem diameter, leaf number, dry weight of aerial parts, number of nodules/plant and percentage of nitrogen. All treatments stimulated germination, growth and BNF, being better in the native strains at a concentration of 108 cells/mL, compared with the commercial strain and the control. These results indicate the positive effect exerted by <em>Rhizobium </em>sp., in promoting growth and BNF, which could be used for further studies to establish the production of a biofertilizer, allowing potentiate the production of crops by gliricidia for food arich cattle as source of protein in the Caribbean Biotechnology Center.</span></span></span></p>

2011

Rhizobium sp, biofertilizante, fijación biológica de nitrógeno, matarratón

Agronomía Colombiana

Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia

Plant ecology