Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
diagnosis, RT-LAMP, rapid detection, COVID-19
Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.
I-Ching Sam, Yee Ling Lau, Afifah Hassan, Pik-Pin Goh, Yee Leng Lee, Meng-Yee Lai, Kalaiarasu M. Peariasamy, Yoong Min Chong, Ilyiana Ismail, Nur Izati Mustapa, Tuan Suhaila Tuan Soh