https://www.socictopen.socict.org/files/original/f4fcb7a0d7b73731b1cf4b661b3d470d.pdf 6c9c586ab8aa1ba87631c3dca37e17c7 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A Novel Method for Detecting p53 Autoantibodies in Sera of Patients with NSCLC Creator An entity primarily responsible for making the resource Kai Tang, Wentao YUE, Yue Wang, Li-na Zhang, Shaofa XU Description An account of the resource Background and objective Serum autoantibody detection is useful means for the early diagnosis and prognosis of cancer. So our objective was to synthesize peptide array to analyse p53 autoantibodies in the sera of patients with non small cell lung cancer (NSCLC). Methods Cellulose-bound overlapping peptides (12 mers) derived from p53 wild type protein were synthesized using SOPTs synthesis technique by an AutoSpot robot –ASP SL (Intavis, Germany). The membrane was incubated with 1/400 dilutions of p53 monoclonal antibody (Sc-53394) to establish a new approach to detect p53 antibody, and the epitopes of the p53 monoclonal antibody is already known. We analysed the p53 autoantibodies from the sera of NSCLC and controls by peptide array and ELISA. Results We synthesized on cellulose membranes twelve-amino-acid overlapping peptides which included all of the sequences of the polypeptide chain of p53. The p53 autoantibody was positive in seven cases of thirty patients’ sera with NSCLC and was negative in sera of the controls, with the same result of ELISA. Conclusion The peptide array could be applied not only to detect the autoantibodies in the sera of patients with lung cancer, but also to map the epitopes of the autoantibodies which might be useful for the early diagnosis and prognosis of cancer. Date A point or period of time associated with an event in the lifecycle of the resource 2009 Subject The topic of the resource Lung neoplasms, p53 gene, epitopes Identifier An unambiguous reference to the resource within a given context DOI: Source A related resource from which the described resource is derived Chinese Journal of Lung Cancer Publisher An entity responsible for making the resource available Chinese Anti-Cancer Association; Chinese Antituberculosis Association Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Neoplasms. Tumors. Oncology. Including cancer and carcinogens Language A language of the resource ZH