https://www.socictopen.socict.org/files/original/0c1f9bd6bac42c3844d338be197b4b0d.pdf 1ee63eb5b0762570a78caf97bed39a30 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Altering SARS Coronavirus Frameshift Efficiency Affects Genomic and Subgenomic RNA Production Creator An entity primarily responsible for making the resource Ewan P. Plant, Deborah R. Taylor, Jonathan D. Dinman, Ralph S. Baric, Amy C. Sims Description An account of the resource In previous studies, differences in the amount of genomic and subgenomic RNA produced by coronaviruses with mutations in the programmed ribosomal frameshift signal of ORF1a/b were observed. It was not clear if these differences were due to changes in genomic sequence, the protein sequence or the frequency of frameshifting. Here, viruses with synonymous codon changes are shown to produce different ratios of genomic and subgenomic RNA. These findings demonstrate that the protein sequence is not the primary cause of altered genomic and subgenomic RNA production. The synonymous codon changes affect both the structure of the frameshift signal and frameshifting efficiency. Small differences in frameshifting efficiency result in dramatic differences in genomic RNA production and TCID50 suggesting that the frameshifting frequency must stay above a certain threshold for optimal virus production. The data suggest that either the RNA sequence or the ratio of viral proteins resulting from different levels of frameshifting affects viral replication. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource SARS, severe acute respiratory syndrome, pseudoknot, ribosomal frameshifting, viral replication Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/v5010279 Source A related resource from which the described resource is derived Viruses Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN