https://www.socictopen.socict.org/files/original/6e77ab2322deedd564080fc8a1714310.pdf c0dc20883f6129646a1b8ea070a2180e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Molecular detection of SARS-CoV-2 from indoor air samples in environmental monitoring needs adequate temporal coverage and infectivity assessment. Creator An entity primarily responsible for making the resource Pierluigi Barbieri, Luisa Zupin, Sabina Licen, Valentina Torboli, Sabrina Semeraro, Sergio Cozzutto, Jolanda Palmisani, Alessia Di Gilio, Gianluigi de Gennaro, Francesco Fontana, Cinzia Omiciuolo, Alberto Pallavicini, Maurizio Ruscio, Sergio Crovella Description An account of the resource The relevance of airborne exposure to SARS-CoV-2 in indoor environments is a matter of research and debate, with special importance for healthcare low-risk settings. Experimental approaches to the bioaerosol sampling are neither standardized nor optimized yet, leading in some cases to limited representativity of the temporal and spatial variability of viral presence in aerosols. Airborne viral viability moreover needs to be assessed. A study has been conducted collecting five 24-h PM10 samples in a COVID-19 geriatric ward in late June 2020, and detecting E and RdRp genes by RT-qPCR with a Ct between 36 and 39. The viral RNA detection at Ct = 36 was related to the maximal numerosity of infected patients hosted in the ward. Lacking a direct infectivity assessment for the collected samples an experimental model has been defined, by seeding twelve nasopharyngeal swab extracts from COVID-19 positive patients on Vero E6 cells; only the four extracts with a viral load above E+10 viral copies (approximately Ct<24) have been able to establish a persistent infection in vitro. Therefore, the cytopathic effect, a key feature of residual infectivity, could be considered unlikely for the environmental PM10 samples showing amplification of viral RNA at Ct = 36 or higher. A standardization of airborne SARS-CoV-2 long-term monitoring and of environmental infectivity assessment is urgently needed. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource SARS-CoV-2, RT-qPCR, Indoor air, Low-risk healthcare unit, Residual infectivity Identifier An unambiguous reference to the resource within a given context 10.1016/j.envres.2021.111200 Source A related resource from which the described resource is derived Environmental research