https://www.socictopen.socict.org/files/original/26869ee46c0ee4d636cde8ce6c6bc546.pdf 4a0a21c6908c315ad2e2175671f92b4c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Determination of Apparent Protein Molecular Weight in Solution by Diffusion Ordered NMR Spectroscopy Creator An entity primarily responsible for making the resource Lihong Wei, YUAN Zhi-liang, XIA Bin Description An account of the resource Diffusion ordered NMR spectroscopy (DOSY) can be used to determine the apparent molecular weight of proteins (Mprotein) by measuring self-diffusion coefficient (Dt-protein). However, Dt-protein is also affected by the property of buffer solution, making the measurements complicated. In this work, 3-(trimethylsilyl) propane sodium sulfonate (DSS) was used as the internal reference molecule and the diffusion coefficient ratio between the target protein and DSS (Dr) was used to characterize the protein in solution. This method reduced the effects of buffer system on Mprotein determination, yielding more accurate Mprotein measurement. The Dr values of reference proteins with different molecular weight were determined. A correlation curve between Dr and Mprotein was established (lgMprotein=-2.6488 lgDr-0.7863, R2=0.997). Finally, Dr of the C-terminal domain of SARS-CoV main protease (Mpro-C) was determined and to yield its molecular weight. Accuracy and practicability of the fitting curve were demonstrated. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource liquid-state NMR, apparent molecular weight, diffusion ordered NMR spectroscopy (DOSY), buffer solution, pulse gradient Identifier An unambiguous reference to the resource within a given context DOI: 10.11938/cjmr20182625 Source A related resource from which the described resource is derived Chinese Journal of Magnetic Resonance Publisher An entity responsible for making the resource available Science Press Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Electricity and magnetism Language A language of the resource ZH